Experimental Methods

Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


Early research into human hair was done primarily on the chemical and physical properties of the hair fiber itself. Key topics dealt with the analysis of chemical composition in the fiber, microstructure, and hair growth, to name a few. Until about 2000, most information about the detailed structure of human hair was obtained from scanning electron microscope (SEM) and transmission electron microscope (TEM) observations (Robbins, 1994; Swift, 1991, 1997; Wei et al., 2005). Mechanical properties were also of interest. Most of the mechanical property measurements of human hair were on the macroscale and used conventional methods, such as tension, torsion, and bending tests (Barnes and Roberts, 2000; Feughelman, 1997; Robbins, 1994; Swift, 1999, 2000; Jachowitz and McMullen, 2002).


Adhesive Force Hair Sample Human Hair Dynamic Contact Angle Hair Surface 
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  1. Robbins C (1994) Chemical and physical behavior of human hair, 3rd edn. Springer, New York, NYGoogle Scholar
  2. Swift JA (1991) Fine details on the surface of human hair. Int J Cosmetic Sci 13:143–159CrossRefGoogle Scholar
  3. Swift JA (1997) Morphology and histochemistry of human hair. In: Jolles P, Zahn H, Hoecker H (eds) Formation and structure of human hair, Birkhäuser Verlag, Berlin, pp 149–175Google Scholar
  4. Wei G, Bhushan B, Torgerson PM (2005) Nanomechanical characterization of human hair using nanoindentation and SEM. Ultramicroscopy 105:155–175CrossRefGoogle Scholar
  5. Barnes HA, Roberts GP (2000) The non-linear viscoelastic behaviour of human hair at moderate extensions. Inter J Cosmet Sci 22:259–264CrossRefGoogle Scholar
  6. Feughelman A (1997) Mechanical properties and structure of alpha-keratin fibres: wool, human hair and related fibres. University of South Wales Press, SydneyGoogle Scholar
  7. Swift JA (1999) The mechanics of fracture of human hair. Inter J Cosmet Sci 21:227–239CrossRefGoogle Scholar
  8. Swift JA (2000) The cuticle controls bending stiffness of hair. J Cosmet Sci 51:37–38ADSGoogle Scholar
  9. Jachowicz J, McMullen R (2002) Mechanical analysis of elasticity and flexibility of virgin and polymer-treated hair fiber assemblies. J Cosmet Sci 53:345–361Google Scholar
  10. Syed AN, Kuhajda A, Ayoub H, Ahmad K, Frank EM (1995) African-American hair: its physical properties and differences relative to Caucasian hair. Cosmet Toilet Mag 110:39–48Google Scholar
  11. Bhushan B (2008a) Nanoscale characterization of human hair and hair conditioners. Prog Mater Sci 53:585–710CrossRefGoogle Scholar
  12. Bhushan B, Chen N (2006) AFM studies of environmental effects on nanomechanical properties and cellular structure of human hair. Ultramicroscopy 106:755–764CrossRefGoogle Scholar
  13. Chen N, Bhushan B (2005) Morphological, nanomechanical and cellular structural characterization of human hair and conditioner distribution using torsional resonance mode in an AFM. J Microscopy 220:96–112CrossRefMathSciNetGoogle Scholar
  14. LaTorre C, Bhushan B (2005b) Nanotribological effects of hair care products and environment on human hair using atomic force microscopy. J Vac Sci Technol A 23:1034–1045CrossRefADSGoogle Scholar
  15. Smith JR, Swift JA (2002) Lamellar subcomponents of the cuticular cell membrane complex of mammalian keratin fibres show friction and hardness contrast by AFM. J Microscopy 206:182–193CrossRefMathSciNetGoogle Scholar
  16. LaTorre C, Bhushan B (2005b) Nanotribological effects of hair care products and environment on human hair using atomic force microscopy. J Vac Sci Technol A 23:1034–1045CrossRefADSGoogle Scholar
  17. LaTorre C, Bhushan B (2006) Investigation of scale effects and directionality dependence on adhesion and friction of human hair using AFM and macroscale friction test apparatus. Ultramicroscopy 106:720–734CrossRefGoogle Scholar
  18. LaTorre C, Bhushan B, Yang JZ, Torgerson PM (2006) Nanotribological effects of silicone type, silicone deposition level, and surfactant type on human hair using atomic force microscopy. J Cosmetic Sci 57:37–56Google Scholar
  19. Lodge RA, Bhushan B (2006a) Surface characterization of human hair using tapping mode atomic force microscopy and measurement of conditioner thickness distribution. J Vac Sci Technol A 24:1258–1269CrossRefGoogle Scholar
  20. Lodge RA, Bhushan B (2007a) Effect of physical wear and triboelectric interaction on surface charges measured by Kelvin probe microscopy. J Colloid Interface Sci 310:321–330CrossRefGoogle Scholar
  21. Lodge RA, Bhushan B (2007b) Surface potential measurement of human hair using Kelvin probe microscopy. J Vac Sci Technol A 25:893–902CrossRefGoogle Scholar
  22. Bhushan B, Goldade AV (2000a) Measurement and analysis of surface potential change during wear of single-crystal silicon (100) at ultralow loads using Kelvin probe microscopy. Appl Surf Sci 157:373–381CrossRefADSGoogle Scholar
  23. Bhushan B, Goldade AV (2000b) Kelvin probe microscopy measurements of surface potential change under wear at low loads. Wear 244:104–117CrossRefGoogle Scholar
  24. DeVecchio D, Bhushan B (1998) Use of a nanoscale Kelvin probe for detecting wear precursors. Rev Sci Instrum 69:3618–3824CrossRefADSGoogle Scholar
  25. Seshadri IP, Bhushan B (2008c) Effect of rubbing and load on nanoscale charging characteristics of human hair characterized by AFM based Kelvin probe. J Colloid Interf Sci 325:580–587CrossRefGoogle Scholar
  26. Wei G, Bhushan B (2006) Nanotribological and nanomechanical characterization of human hair using a nanoscratch technique. Ultramicroscopy 106:742–754CrossRefGoogle Scholar
  27. Nikiforidis G, Balas C, Tsambaos D (1992) Mechanical parameters of human hair: possible applications in the diagnosis and follow-up of hair disorders. Clin Phys Physiol Meas 13:281–290CrossRefGoogle Scholar
  28. Swanbeck G, Nyren J, Juhlin L (1970) Mechanical properties of hair from patients with different types of hair diseases. J Invest Dermatol 54:248–251CrossRefGoogle Scholar
  29. Henderson GH, Karg GM, O’Neill JJ (1978) Fractography of human hair. J Soc Cosmet Chem 29:449–467Google Scholar
  30. Seshadri IP, Bhushan B (2008a) In-situ tensile deformation characterization of human hair with atomic force microscopy. Acta Mater 56:774–781CrossRefGoogle Scholar
  31. Seshadri IP, Bhushan B (2008b) Effect of ethnicity and treatments on in situ tensile response and morphological changes of human hair characterized by atomic force microscopy. Acta Mater 56:3585–3597CrossRefGoogle Scholar
  32. Bhushan B (1999a) Principles and applications of tribology. Wiley, New York, NYGoogle Scholar
  33. Bhushan B (1999b) Handbook of micro/nanotribology. 2nd edn. CRC Press, Boca Raton, FLGoogle Scholar
  34. Bhushan B (2002) Introduction to tribology. Wiley, New York, NYGoogle Scholar
  35. Bhushan B (2008b) Nanotribology and nanomechanics – an introduction, 2nd edn. Springer, HeidelbergGoogle Scholar
  36. Bhushan B, Li X (2003) Nanomechanical properties of solid surfaces and thin films (invited). Inter Mater Rev 48:125–164CrossRefGoogle Scholar
  37. Li X, Bhushan B, McGinnis PB (1996) Nanoscale mechanical characterization of glass fibers. Mater Lett 29:215–220CrossRefGoogle Scholar
  38. Parbhu AN, Bryson WG, Lal R (1999) Disulfide bonds in the outer layer of keratin fibers confer higher mechanical rigidity: correlative nano-indentation and elasticity measurement with an AFM. Biochemistry 38:11755–11761CrossRefGoogle Scholar
  39. Kasai T, Bhushan B, Huang L, Su CM (2004) Topography and phase imaging using the torsional resonance mode. Nanotechnology 15:731–742CrossRefADSGoogle Scholar
  40. Bhushan B, Kasai T (2004) A surface topography-independent friction measurement technique using torsional resonance mode in an AFM. Nanotechnology 15:923–935CrossRefADSGoogle Scholar
  41. Scott WW, Bhushan B (2003) Use of phase imaging in atomic force microscopy for measurement of viscoelastic contrast in polymer nanocomposites and molecularly thick lubricant films. Ultramicroscopy 97:151–169CrossRefGoogle Scholar
  42. Song Y, Bhushan B (2005) Quantitative extraction of in-plane surface properties using torsional resonance mode of atomic force microscopy. J Appl Phys 97:083533CrossRefADSGoogle Scholar
  43. Bobji MS, Bhushan B (2001a) In situ microscopic surface characterization studies of polymeric thin films during tensile deformation using atomic force microscopy. J Mater Res 16:844–855CrossRefADSGoogle Scholar
  44. Bobji MS, Bhushan B (2001b) Atomic force microscopy study of the microcracking of magnetic thin films under tension. Scripta Mater 44:37–42CrossRefGoogle Scholar
  45. Tambe NS, Bhushan B (2004) In situ study of nano-cracking in multilayered magnetic tapes under monotonic and fatigue loading using an AFM. Ultramicroscopy 100:359–373CrossRefGoogle Scholar
  46. Bhushan B, Wei G, Haddad P (2005) Friction and wear studies of human hair and skin. Wear 259:1012–1021CrossRefGoogle Scholar
  47. Chen N, Bhushan B (2006) Atomic force microscopy studies of conditioner thickness distribution and binding interactions on the hair surface. J Microscopy 221:203–215CrossRefMathSciNetGoogle Scholar
  48. Bhushan B, Dandavate C (2000) Thin-film friction and adhesion studies using atomic force microscopy. J Appl Phys 87:1201–1210CrossRefADSGoogle Scholar
  49. Lodge RA, Bhushan B (2006b) Wetting properties of human hair By Means Of dynamic contact angle measurement. J Appl Poly Sci 102:5255–5265CrossRefGoogle Scholar
  50. Jalbert C, Koberstein JT, Yilgor I, Gallagher P, Krukonis V (1993) Molecular weight dependence and end-group effects on the surface tension of poly(dimethylsiloxane). Macromolecules 26:3069–3074CrossRefADSGoogle Scholar
  51. Lerebour G, Cupferman S, Cohen C, Bellon-Fontaine MN (2000) Comparison of surface free energy between reconstructed human epidermis and in situ human skin. Skin Res Technol 6:245–249CrossRefGoogle Scholar
  52. Schott H (1971) Contact angles and wettability of human skin. J Pharm Sci 60:1893–1895CrossRefGoogle Scholar
  53. Ginn ME, Noyes CM, Jungermann E (1968) The contact angle of water on viable human skin. J Colloid Interface Sci 26:146–151CrossRefGoogle Scholar
  54. Tao Z, Bhushan B (2006a) Surface modification of AFM Si3N4 probes for adhesion/friction reduction and imaging improvement. ASME J Tribol 128:865–875CrossRefGoogle Scholar
  55. Yanazawa H (1984) Adhesion model and experimental-verification for polymer SIO2 system. Colloids Surf 9:133–145CrossRefGoogle Scholar
  56. Tao Z, Bhushan B (2006b) Wetting properties of AFM probes By Means Of contact angle measurements. J Phys Appl Phys 39:3858–3862ADSGoogle Scholar
  57. Molina R, Comelles F, Julia MR, Erra P (2001) Chemical modifications on human hair studied by means of contact angle determination. J Colloid Interface Sci 237:40–46CrossRefGoogle Scholar
  58. Bhushan B, Burton Z (2005) Adhesion and friction properties of polymers in microfluidic devices. Nanotechnology 16:467–478CrossRefADSGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Nanoprobe Laboratory for Bio- and Nanotechnology and Biomimetics (NLB2)Ohio State UniversityColumbusUSA

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