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Optical Fluorescence Microscopy pp 165–181Cite as

In Vitro–In Vivo Fluctuation Spectroscopies

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Abstract

Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.

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Notes

  1. 1.

    We have used the relation: \( {\left| {{{\hat{W}}_{DB}}({\mathbf{q}},{\mathbf{R}})} \right|^2} = 2{\left| {\hat{W}({\mathbf{q}})} \right|^2}\left( {1 + \cos \left[ {{\mathbf{q}} \cdot {\mathbf{R}}} \right]} \right) \)

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Acknowledgments

We acknowledge the Fondazione Cariplo (fund 2005-1079 to G.C.) and the MIUR Prin fund (2006027587 to G.C.).

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Authors and Affiliations

  1. Dipartimento di Fisica G.Occhialini, Università di Milano Bicocca, Piazza della Scienza 3, 20126, Milan, Italy

    M. Collini, L. D’Alfonso, M. Caccia, L. Sironi, M. Panzica & G. Chirico

  2. Dipartimento di Medicina Sperimentale, Università di Milano Bicocca, Via Cadore 48, 20052, Monza, Italy

    I. Rivolta, B. Lettiero & G. Miserocchi

Authors
  1. M. Collini
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  2. L. D’Alfonso
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  3. M. Caccia
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  4. L. Sironi
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  5. M. Panzica
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  6. G. Chirico
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  7. I. Rivolta
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  8. B. Lettiero
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  9. G. Miserocchi
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Corresponding author

Correspondence to G. Chirico .

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Editors and Affiliations

  1. , Nanophysics Unit, The Italian Institute of Technology - II, Via Morego 30, Genova, 16163, Italy

    Alberto Diaspro

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Collini, M. et al. (2011). In Vitro–In Vivo Fluctuation Spectroscopies. In: Diaspro, A. (eds) Optical Fluorescence Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15175-0_10

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