In vitro and in vivo Examinations for Detection of Minimal Infective Dose for Biomaterials
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The objective of the study is to examine the minimal infective dose of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomaterials in vivo and in vitro. In this research originally synthesized bioactive biomaterials – 4N glass and 4NK glass-ceramics are calcium phosphate with Na2O and Nb2O5 additives with different crystallinity. For microbiological investigation of biomaterialsS.epidermidis strain ATCC 12228, Ps.aeruginosa strain ATCC 27853, 4 rabbit animal models, broth and agar, scanned electron microscope Tescan, Vortex centrifuge, sonicator and histological methods were used. We observed that different biomaterials had various attachments of S.epidermidis and Ps.aeruginosa in laboratory animal models. The minimal infective dose of S.epidermidis for biomaterial 4NK is more than 103 CFU/ml, for biomaterial 4N less than 103 CFU/ml. The minimal infective dose of Ps.aeruginosa for biomaterial 4N and biomaterial 4NK is less than 103 CFU/ml.
Keywordsbiomaterials adhesion Pseidomonas aeruginosa Staphylococcus aureus
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