Skip to main content
SpringerLink
Log in

Fluorescence and Light Scatter Experiments to Investigate Cell Properties at Microgravity

  • Chapter
Self-organization and Pattern-formation in Neuronal Systems Under Conditions of Variable Gravity

Part of the book series: Nonlinear Physical Science ((NPS))

  • 351 Accesses

Abstract

Optical methods have been developed to measure a variety of biological relevant parameters of cells. Especially fluorescent dyes are meanwhile available for an increasing number of interesting features. In the described experiments we were mainly interested in dyes for membrane potential and variety of dyes for ionic concentrations. The intracellular calcium concentration is of specific interest here. Another question is that about changes in size and geometry of cells under variable gravity conditions, here light scatter experiments have been proven to be very useful. In the following both types of experiments will be presented, using the Bremen drop-tower as a microgravity platform.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Frisken B.J., 2001, Revisiting the method of cumulants for the analysis of dynamic light-scattering data, Applied Optics, 40, 4087–4091.

    Article  ADS  Google Scholar 

  • Haugland R.P., 2005, The handbook: A guide to fluorescent probes and labeling technologies, Molecular Probes (Invitrogen), Eugene, USA.

    Google Scholar 

  • Meissner K., Piqueira J.R.C. and Hanke W., 2004, Flourescent and dispersion experiments on biological membranes under microgravity, J. Gravitational Physiol., II(2), 195–196.

    Google Scholar 

  • Pecora R., 1985, Dynamic Light Scattering, Plenum, New York.

    Book  Google Scholar 

  • Vaughn J.L., Goodwin R.H., Tompkins G.J. and McCawley P., 1977, The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae), Vitro, 13, 213–217.

    Article  Google Scholar 

  • Weiss T.F., 1997, Cellular Biophysics: Eelectrical properties, The MIT Press, Cambridge, MA, USA.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology (230), University of Hohenheim Membramephysiology, Garbenstrasse 30, 70593, Stuttgart, Germany

    Meike Wiedemann, Florian P. M. Kohn & Wolfgang R. L. Hanke

  2. Department of Zoology, University of Hohenheim, Garbenstrasse 30, 70593, Stuttgart, Germany

    Harald Roesner

Authors
  1. Meike Wiedemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Florian P. M. Kohn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Harald Roesner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wolfgang R. L. Hanke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Higher Education Press, Beijing and Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Wiedemann, M., Kohn, F.P.M., Roesner, H., Hanke, W.R.L. (2011). Fluorescence and Light Scatter Experiments to Investigate Cell Properties at Microgravity. In: Self-organization and Pattern-formation in Neuronal Systems Under Conditions of Variable Gravity. Nonlinear Physical Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14472-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-14472-1_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-14471-4

  • Online ISBN: 978-3-642-14472-1

Publish with us

Policies and ethics

Search

Navigation