Poly(n-butylcyanoacrylate) Submicron Particles Loaded with Ciprofloxacin for Potential Treatment of Bacterial Infections

  • Georgi YordanovEmail author
  • Nikola Abrashev
  • Ceco Dushkin
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 137)


Poly(n-butylcyanoacrylate) submicron colloidal particles loaded with the antibiotic ciprofloxacin are prepared by emulsion polymerization and characterized by scanning electron microscopy, dynamic light scattering, nuclear magnetic resonance and gel-permeation chromatography. The entrapment efficiency for ciprofloxacin in the polymer particles is investigated as a function of the monomer and drug concentrations in the polymerization medium. The kinetics of ciprofloxacin release from the polymer particles is found to depend on the pH of the release medium. Studies by gel-permeation chromatography indicate for a possible drug-polymer association. The antibacterial activity of the obtained formulation is tested on a clinical isolate of Escherichia coli bacterium and is found to be similar to that of the free drug.


poly(n-butylcyanoacrylate) colloids submicron particles ciprofloxacin drug release Escherichia coli 



G.Y. and C.D. are thankful to COST Action D43 of the European Community.


  1. 1.
    Salata OV (2004) J Nanobiotechnology 2: 3.CrossRefGoogle Scholar
  2. 2.
    Barratt G (2003) Cell Mol Life Sci 60: 21-37.CrossRefGoogle Scholar
  3. 3.
    Brannon-Peppas L, Blanchette JO (2004) Adv Drug Deliver Rev 56: 1649-1659.CrossRefGoogle Scholar
  4. 4.
    Briones E, Colino CI, Lanao J (2008) J Controlled Release 125: 210-227.CrossRefGoogle Scholar
  5. 5.
    Gulyaev A, Ermekbaeva B, Kivman G, Radchenko T, Sherstov A, Shirinskii V (1998) Pharm Chem J 32: 3.Google Scholar
  6. 6.
    Pinto-Alphandary H, Andremont A, Couvreur P (2000) Int J Antimicrob Ag 13: 155-168.CrossRefGoogle Scholar
  7. 7.
    Vauthier C, Dubernet C, Fattal E, Pinto-Alphandary H, Couvreur P (2003) Adv Drug Deliver Rev 55: 519–548.CrossRefGoogle Scholar
  8. 8.
    Fattal E, Youssef M, Couvreur P, Andremont A (1989) Antimicrob Agents Chemotherapy 33: 1540-1543.CrossRefGoogle Scholar
  9. 9.
    Balland O, Pinto-Alphandary H, Pecquet S, Andremont A, Couvreur P (1994) J Antimicrob Chemother 33: 509–522.CrossRefGoogle Scholar
  10. 10.
    Kisich K, Gelperina S, Higgins M, Wilson S, Shipulo E, Oganesyan E, Heifets L (2007) Int J Pharm 345: 154–162.CrossRefGoogle Scholar
  11. 11.
    Zhang Q, Liao G, Wei D, Nagai T (1998) Int J Pharm 164: 21–27.CrossRefGoogle Scholar
  12. 12.
    Alonso M, Losa C, Calvo P, Vila-Jato J (1991) Int J Pharm 68: 69-76.CrossRefGoogle Scholar
  13. 13.
    Gonzalez-Martin G, Figueroa C, Merino I, Osuna A (2000) Eur J Pharm Biopharm 49: 137-142.CrossRefGoogle Scholar
  14. 14.
    Fontana G, Licciardi M, Mansueto S, Schillaci D, Giammona G (2001) Biomaterials 22: 2857-2865.CrossRefGoogle Scholar
  15. 15.
    Cavallaro G, Fresta M, Giammona G, Puglisi G, Villari A (1994) Int J Pharm 111: 31-41.CrossRefGoogle Scholar
  16. 16.
    Appelbaum P, Hunter P (2000) Int J Antimicrob Ag 16: 5–15.CrossRefGoogle Scholar
  17. 17.
    Kumar PV, Jain1 NK (2007) AAPS PharmSciTech 8(1): E1-E6.Google Scholar
  18. 18.
    Dillen K, Vandervoort J, Van den Mooter G, Verheyden L, Ludwig A (2004) Int J Pharm 275: 171–187.CrossRefGoogle Scholar
  19. 19.
    Dillen K, Vandervoort J, Van den Mooter G, Verheyden L, Ludwig A (2006) Int J Pharm 314: 72–82.CrossRefGoogle Scholar
  20. 20.
    Jeong YI, Na HS, Seo DH, Kim DG, Lee HC, Jang MK, Na SK, Roh SH, Kim SI, Nah JW (2008) Int J Pharm 352: 317–323.CrossRefGoogle Scholar
  21. 21.
    Fawaz F, Guyot M, Lagueny A, Devissaguet J (1997) Int J Parm 154: 191-203.CrossRefGoogle Scholar
  22. 22.
    Fawaz F, Bonini F, Maugein J, Lagueny A (1998) Int J Pharm 168: 255–259.CrossRefGoogle Scholar
  23. 23.
    Page-Clisson M, Gibaud S, Pinto-Alphandary H, Weingarten C, Couvreur P (1998) Int J Pharm 166: 117–120.CrossRefGoogle Scholar
  24. 24.
    Page-Clisson M, Pinto-Alphandary H, Ourevitch M, Andremont A, Couvreur P (1998) J Control Release 56: 23–32.CrossRefGoogle Scholar
  25. 25.
    Oowaki H, Matsuda S, Sakai N, Ohta T, Iwata H, Sadato A, Taki W, Hashimoto N, Ikada Y (2000) Biomaterials 21: 1039–1046.CrossRefGoogle Scholar
  26. 26.
    Donnelly E, Johnston D, Pepper D, Dunn D (1977) J Polym Sci Polym Lett Ed 15: 399–405.CrossRefGoogle Scholar
  27. 27.
    Behan N, Birkinshaw C (2000) Macromol Rapid Commun 21: 884–886.CrossRefGoogle Scholar
  28. 28.
    Behan N, Birkinshaw C, Clarke N (2001) Biomaterials 22: 1335–1344.CrossRefGoogle Scholar
  29. 29.
    Couvreur P, Kante B, Roland M, Speiser P (1979) J Pharm Sci 68: 1521-1524.CrossRefGoogle Scholar
  30. 30.
    Vansnick L, Couvreur P, Christiaensleyh D, Roland M (1985) Pharm Res 1: 36–41.CrossRefGoogle Scholar
  31. 31.
    El-Egakey M, Bentele V, Kreuter J (1983) Int J Pharm 13: 349–352.CrossRefGoogle Scholar
  32. 32.
    Lescure F, Zimmer C, Roy D, Couvreur P (1992) J Colloid Interface Sci 154: 77–86.CrossRefGoogle Scholar
  33. 33.
    Douglas S, Davis S, Holding S (1985) Br Polym J 17: 339–342.CrossRefGoogle Scholar
  34. 34.
    Alonso M, Sanchez A, Torres D, Seijo B (1990) J Microencapsul 7: 517–526.CrossRefGoogle Scholar
  35. 35.
    Barron D, Jimenez-Lozano E, Irles A, Barbosa J (2000) J Chromatography A 871: 381–389.CrossRefGoogle Scholar
  36. 36.
    Barbosa J, Barron D, Jimenez-Lozano E, Sanz-Nebot V (2001) Anal Chim Acta 437: 309–321.CrossRefGoogle Scholar
  37. 37.
    Vezin W, Florence A (1980) J Biomed Mater Res 14: 93-106.CrossRefGoogle Scholar
  38. 38.
    Lenaerts V, Couvreur P, Christiaens-Leyh D, Joiris E, Roland M, Rollman B, Speiser P (1984) Biomaterials 5: 65-68.CrossRefGoogle Scholar
  39. 39.
    Muller R, Lherm C, Herbort J, Couvreur P (1990) Biomaterials 11: 590-595.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Georgi Yordanov
    • 1
    Email author
  • Nikola Abrashev
    • 2
  • Ceco Dushkin
    • 1
  1. 1.Laboratory of Nanoparticle Science and Technology, Department of General and Inorganic ChemistryFaculty of Chemistry, Sofia University ‘St.Kliment Ohridski’SofiaBulgaria
  2. 2.Microbiological LaboratoryMultiprofile Hospital for Active Treatment ‘Dr. Nikola Vasiliev’KyustendilBulgaria

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