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Genetic Modification in Dedicated Bioenergy Crops and Strategies for Gene Confinement

  • Albert P. KauschEmail author
  • Joel Hague
  • Melvin Oliver
  • Yi Li
  • Henry Daniell
  • Peter Mascia
  • C. Neal StewartJr
Chapter
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 66)

Abstract

Genetic modification of dedicated bioenergy crops is in its infancy; however, there are numerous advantages to the use of these tools to improve crops used for biofuels. Potential improved traits through genetic engineering (GE) include herbicide resistance, pest-, drought-, cold- and salt-tolerance, lower inputs, compositional alterations, addition of cellulases and other biofuels-specific traits such as increased biomass yields and increased photosynthetic efficiencies. To achieve these goals on an agricultural scale, these improvements must meet regulatory standards for release into the environment. In most cases, these criteria will probably require gene confinement strategies to prevent gene flow into wild and non-transgenic populations. Here, we consider the options for prevention or mitigation of gene flow in genetically modified (GM) biofuels crops.

Keywords

Genetically Modify Cytoplasmic Male Sterilty Perennial Grass Late Embryogenesis Abundant Biofuel Crop 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Albert H, Dale EC, Lee E, Ow D (1995) Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J 7:649–659PubMedCrossRefGoogle Scholar
  2. Altieri M (2000) The ecological impacts of transgenic crops on agroecosystem health. Ecosyst Health 6:13–23Google Scholar
  3. Bayley CC, Morgan M, Dale EC, Ow D (1992) Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Mol Biol 18:353–361PubMedCrossRefGoogle Scholar
  4. Belanger FC, Meagher TR, Day PR, Plumley K, Meyer WA (2003) Interspecific hybridization between Agrostis stolonifera and related Agrostis species under field conditions. Crop Sci 43:240–246CrossRefGoogle Scholar
  5. Boffey TB, Veevers A (1977) Balanced designs for two-component competition experiments. Euphytica 26:481–484CrossRefGoogle Scholar
  6. Bradford KJ, Van Deynze A, Gutterson N, Parrott W, Strauss SH (2005) Regulating transgenic crops sensibly: lessons from plant breeding, biotechnology and genomics. Nat Biotechnol 23:439–444PubMedCrossRefGoogle Scholar
  7. Dale EC, Ow DW (1990) Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91:79–85PubMedCrossRefGoogle Scholar
  8. Dale EC, Ow DW (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88:10558–10562PubMedCrossRefGoogle Scholar
  9. Dale PJ (1993) The release of transgenic plants into agriculture. J Agric Sci 120:1–5CrossRefGoogle Scholar
  10. Daniell H (2002) Molecular strategies for gene containment in transgenic crops. Nat Biotechnol 20:581–586PubMedCrossRefGoogle Scholar
  11. De Block M, Debrouwer D, Moens T (1997) The development of nuclear male sterility system in wheat: expression of the barnase gene under the control of tapetum specific promoters. Theor Appl Genet 95:125–131CrossRefGoogle Scholar
  12. De Cosa B, Moar W, Lee SB, Miller M, Daniell H (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19:71–74PubMedCrossRefGoogle Scholar
  13. Dunwell J, Ford CS (2005) Technologies for biological containment of GM and non-GM crops. DEFRA Contract CPEC 47, http://www.gmo-safety.eu/pdf/biosafenet/Defra_2005.pdf Google Scholar
  14. Goetz M, Godt DE, Guivarc’h A, Kahmann U, Chriqui D, Roitsch T (2001) Induction of male sterility in plants by metabolic engineering of the carbohydrate supply. Proc Natl Acad Sci USA 98:6522–6527PubMedCrossRefGoogle Scholar
  15. Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP (1989) Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1:173–179PubMedGoogle Scholar
  16. Hartley RW (1988) Barnase and Barstar: expression of its cloned inhibitor permits expression of a cloned ribonuclease. J Mol Biol 202:913–915PubMedCrossRefGoogle Scholar
  17. He S, Abad AR, Gelvin SB, Mackenzie SA (1996) A cytoplasmic male sterility-associate mitochondrial protein causes pollen disruption in transgenic tobacco. Proc Natl Acad Sci USA 93:11763–11768PubMedCrossRefGoogle Scholar
  18. Higginson T, Li SF, Parish RW (2003) AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana. Plant J 35:177–192PubMedCrossRefGoogle Scholar
  19. Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Kumar P, Pental D (2001) The use of spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breed 8:11–23CrossRefGoogle Scholar
  20. Lee J-Y, Aldemita RR, Hodges TK (1996) Isolation of a tapetum-specific gene and promoter from rice. Int Rice Res Newsl 21:2–3Google Scholar
  21. Luo H, Lyznik LA, Gidoni D, Hodges TK (2000) FLP-mediated recombination for use in hybrid plant production. Plant J 23:423–430PubMedCrossRefGoogle Scholar
  22. Luo K, Duan H, Zhao D, Zheng X, Deng W, Chen Y, Stewart CN, McAvoy R, Jiang X, Wu Y, He A, Pei Y, Li Y (2007) "GM-gene-deletor": fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnol J 5:263–374PubMedCrossRefGoogle Scholar
  23. Mallory-Smith C, Zapiola ML (2008) Gene flow from glyphosate-resistant crops. Pest Manag Sci 64:428–440PubMedCrossRefGoogle Scholar
  24. Mariani C, Beuckeleer M, Truettner J, Leemans J, Goldberg R (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 374:737–738CrossRefGoogle Scholar
  25. Moffatt B, Somerville C (1988) Positive selection for male-sterile mutants of Arabidopsis lacking adenine phosphoribosyl transferase activity. Plant Physiol 86:1150–1154PubMedCrossRefGoogle Scholar
  26. Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1998) Control of plant gene expression. US Patent 5723765. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5723765.html Google Scholar
  27. Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1999a) Control of plant gene expression. US Patent 5977441. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5977441.html Google Scholar
  28. Oliver MJ, Quisenberry JE, Trolinder N, Glover L, Keim DL (1999b) Control of plant gene expression. US Patent 5925808. United States Patent and Trademarks Office, http://www.patentstorm.us/patents/5925808.html Google Scholar
  29. Reichman JR, Watrud LS, Lee EH, Burdick CA, Bollman MA, Storm MJ, King GA, Mallory-Smith C (2006) Establishment of transgenic herbicide-resistant creeping bentgrass (Agrostis stolonifera L.) in nonagronomic habitats. Mol Ecol 15:4243–4255PubMedCrossRefGoogle Scholar
  30. Robertson GP, Dale VH, Doering OC, Hamburg SP, Melillo JM, Wander MM, Parton WJ, Adler PR, Barney JN, Cruse RM, Duke CS, Fearnside PM, Follett RF, Gibbs HK, Goldemberg J, Mladenoff DJ, Ojima D, Palmer MW, Sharpley A, Wallace L (2008) Sustainable biofuels redux. Science 322:49–50PubMedCrossRefGoogle Scholar
  31. Ruf S, Karcher D, Bock R (2007) Determining the transgene containment level provided by chloroplast transformation. Proc Natl Acad Sci USA 104:6998–7002PubMedCrossRefGoogle Scholar
  32. Snow AA, Moran Palma P (1997) Commercialization of transgenic plants: potential ecological risks. BioScience 47:86–96CrossRefGoogle Scholar
  33. Stegemann S, Hartmann S, Ruf S, Bock R (2003) High-frequency gene transfer from the chloroplast genome to the nucleus. Proc Natl Acad Sci USA 100:8828–8833PubMedCrossRefGoogle Scholar
  34. Stewart CN (2007) Biofuels and biocontainment. Nat Biotechnol 25:283–284PubMedCrossRefGoogle Scholar
  35. Sticklen MB (2008) Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat Rev Genet 9:433–443PubMedCrossRefGoogle Scholar
  36. Theerakulpisut P, Xu H, Singh MB, Pettitt JM, Knox RB (1991) Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris. Plant Cell 3:1073–1084PubMedGoogle Scholar
  37. Tomes DT, Huang B, Miller PD (1998) Genetic constructs and methods for producing fruits with very little or diminished seed. US Patent 5773697, http://www.freepatentsonline.com/5773697.html Google Scholar
  38. Tsuchiya T, Toriyama K, Yoshikawa M, Ejiri S, Hinata K (1995) Tapetum-specific expression of the gene for an endo-beta-1,3-glucanase causes male sterility in transgenic tobacco. Plant Cell Physiol 36:487–494PubMedGoogle Scholar
  39. Twell D, Wing R, Yamaguchi J, McCormick S (1989) Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet 217:240–245PubMedCrossRefGoogle Scholar
  40. Watrud LS, Lee EH, Fairbrother A, Burdick C, Reichman JR, Bollman M, Storm M, King G, van De Waters, PK (2004) Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proc Natl Acad Sci USA 101:14533–14538PubMedCrossRefGoogle Scholar
  41. Wipff JK, Fricker C (2001) Gene flow from transgenic creeping bentgrass (Agrostis stolonifera L.) in the Willamette Valley, Oregon. Int Turfgrass Soc Res J 9:224–242Google Scholar
  42. Xu H, Knox RB, Taylor PE, Singh MB (1995) Bcp1, a gene required for male fertility in Arabidopsis. Proc Natl Acad Sci USA 92:2106–2110PubMedCrossRefGoogle Scholar
  43. Yui R, Iketani S, Mikami T, Kubo T (2003) Antisense inhibition of mitochondrial pyruvate dehydrogenase E1α subunit in anther tapetum causes male sterility. Plant J 34:57–66PubMedCrossRefGoogle Scholar
  44. Zapiola ML, Campbell CK, Butler MD, Mallory-Smith CA (2008) Escape and establishment of transgenic glyphosate-resistant creeping bentgrass Agrostis stolonifera in Oregon, USA: a 4-year study. J Appl Ecol 45:486–494CrossRefGoogle Scholar
  45. Zapiola ML, Mallory-Smith CA, Thompson JH, Rue LJ, Campbell CK, Butler MD (2007) Gene escape from glyphosate-resistant creeping bentgrass fields: past, present, and future. Proceedings of the 60th Meeting of the Western Society of Weed Science, 13–15 March 2007, Portland, OR, Abstract 82Google Scholar
  46. Zuo J, Chua NH (2000) Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotechnol 11:146–151PubMedCrossRefGoogle Scholar
  47. Zuo J, Niu QW, Chua NH (2000) An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J 24:265–273PubMedCrossRefGoogle Scholar
  48. Zou JT, Zhan XY, Wu HM, Wang H, Cheung AY (1994) Characterization of a rice pollen-specific gene and its expression. Am J Bot 81:552–561CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Albert P. Kausch
    • 1
    Email author
  • Joel Hague
    • 1
  • Melvin Oliver
    • 2
  • Yi Li
    • 3
  • Henry Daniell
    • 4
  • Peter Mascia
    • 5
  • C. Neal StewartJr
    • 6
  1. 1.University of Rhode IslandWest KingstonUSA
  2. 2.USDA-ARS Plant Genetics UnitUniversity of MissouriColumbiaUSA
  3. 3.University of ConnecticutStorrsUSA
  4. 4.Biomolecular ScienceCentral Florida State UniversityOrlandoUSA
  5. 5.CeresThousand OaksUSA
  6. 6.Racheff Chair of Excellence in Plant Molecular GeneticsUniversity of TennesseeKnoxvilleUSA

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