Application of Shrimp Chitosan Solution as Additive and Supplementing Ingredient in Culturing 3T3 Fibroblast Cells

Abstract

In the present study we show that the improved proliferation of the 3T3 fibroblast cell line and the cells attachment could be obtained when applied a suitable chitosan solution as additive and supplementing ingredient in the cell culture medium of DMEM with 10% FBS, 50 U/ml Penicillin, 50 μg/ml Streptomycin, 50 μg/ml Neomycin. In addition, the 3T3 Fibroblast cells formed clear cell colonies in the cultured medium. By applying a concentration of chitosan solution of 35ppm with a volume ratio of 1: 3 to the standard cell culture medium, the cell growth of the 3T3 cell line was increased up to about 33.33% comparing to medium without chitosan solution.