Regulations of Cell Division from Streptomyces That May Play an Important Role in Drug Resistance
A gene minDSL encoding a cell division of D-cycloserine producing Streptomyces has been cloned and sequenced . The minDSL gene was expressed in E. coli. The minDSL gene gives rise to a filamentous phenotype in E. coli when MinDSL protein was fused with His-tag in pET21a(+) construction. Scanning electron microscope analysis shows the indentations along the cells. His-tag MinDSL protein is soluble. However, the introduction of minDSL gene with the sequence containing 49 nucleotides at up stream of minDSL gene gave apparent morphological alteration into minicells when using pET28a(+) as an expressed vector. The native MinDSL protein is insoluble. In this study, we showed morphological diferentiayion, how to purify the insoluble MinDSL protein and D-cycloserine resistant assay. Investigation of minicell production indicates that the production of minicells is blocked when maltose was added in the culture. This is the report about the effects of Streptomyces minDSL gene in E. coli in the formation of minicell phenotype which may participate in drug resistance that is interested in cancer treatment and vaccine preparation nowadays.
Keywordscell division inhibitor Streptomyces drug resistance morphological change
Unable to display preview. Download preview PDF.