Glass Nanopipette: Fabrication and Application for Studying Living Cells
In this paper, we show fabrication of glass nanopipettes by laser heating and pulling. Then the nanopipette is used as the probe of scanning force microscope for studying living cells. We can get topographical image and mechanical properties of cells. Inside the nanopipette we can fulfill the liquid and metal wire for making an electrode. This electrode can be used for measuring ion current of channels through cell membrane by patch-clamp method.
Keywordsnanopipette laser pulling living cell scanning probe microscope (SPM) patch-clamp
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