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Glass Nanopipette: Fabrication and Application for Studying Living Cells

  • Duong Chi Dung
  • Huynh Luong Nghia
  • V. P. Veiko
  • A. O. Golubok
  • E. B. Yakovlev
Part of the IFMBE Proceedings book series (IFMBE, volume 27)

Abstract

In this paper, we show fabrication of glass nanopipettes by laser heating and pulling. Then the nanopipette is used as the probe of scanning force microscope for studying living cells. We can get topographical image and mechanical properties of cells. Inside the nanopipette we can fulfill the liquid and metal wire for making an electrode. This electrode can be used for measuring ion current of channels through cell membrane by patch-clamp method.

Keywords

nanopipette laser pulling living cell scanning probe microscope (SPM) patch-clamp 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Duong Chi Dung
    • 1
  • Huynh Luong Nghia
    • 1
  • V. P. Veiko
    • 2
  • A. O. Golubok
    • 2
  • E. B. Yakovlev
    • 2
  1. 1.Le Quy Don Technical UniversityHanoiVietnam
  2. 2.Saint-Petersburg State University of Information Technologies, Mechanics and OpticsSaint-PetersburgRussia

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