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Stem Cell Engineering pp 159–179Cite as

Genetic Modification of Human Embryonic and Induced Pluripotent Stem Cells: Viral and Non-viral Approaches

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Abstract

Human embryonic stem cells (hESCs) and induced pluripotent cells (iPSCs) are well suited for translational cell therapy. For hESC, the pluripotent phenotype, naturally occurring within the inner cell mass of the early embryo, bestows the capability to differentiate into any cell type of interest and this, coupled with their ability to remain in an undifferentiated state with indefinite proliferative capacity, means that essentially unlimited numbers of identical, well-defined and genetically characterised stem cells can be produced in culture for therapeutic applications. An understanding of the regulatory mechanisms responsible for pluripotency and differentiation potential of hESCs is critical for translating their potential in vitro to therapeutic use in vivo. Harnessing of this therapeutic potential in conjunction with modern genetic modification tools promises great advancement in the study of developmental and adult physiology and pathophysiology with a view towards implementation of genetically modified hESCs to advance regenerative medicine.

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Acknowledgments

The authors thank S. Mukherjee (Institute for Child Health, London) for help with Fig. 3.

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Authors and Affiliations

  1. Faculty of Medicine, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK

    Nicole M. Kane & Andrew H. Baker

  2. Wolfson Centre for Stem Cells, Tissue Engineering & Modelling (STEM), Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK

    Chris Denning

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  1. Nicole M. Kane
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Correspondence to Andrew H. Baker .

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Editors and Affiliations

  1. FH Aachen, Standort Jülich, Ginsterweg 1, Jülich, 52428, Germany

    Gerhard M. Artmann

  2. Wolfson Centre for Age-Related Diseases, GKT School of Biomedical Sciences, King's College London, London, SE11UL, United Kingdom

    Stephen Minger

  3. Inst. Neurophysiologie, Universitätsklinikum Köln, Robert-Koch-Str. 39, Köln, 50931, Germany

    Jürgen Hescheler

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Kane, N.M., Denning, C., Baker, A.H. (2011). Genetic Modification of Human Embryonic and Induced Pluripotent Stem Cells: Viral and Non-viral Approaches. In: Artmann, G., Minger, S., Hescheler, J. (eds) Stem Cell Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-11865-4_7

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