Abstract
Since the inception of the microscope, optical imaging is serving the biological discovery for more than four centuries. With the recent emergence of methods appropriate for in vivo staining, such as bioluminescence, fluorescent molecular probes, and proteins, as well as nanoparticle-based targeted agents, significant attention has been shifted toward in vivo interrogations of different dynamic biological processes at the molecular level. This progress has been largely supported by the development of advanced optical tomographic imaging technologies suitable for obtaining volumetric visualization of biomarker distributions in small animals at a whole-body or whole-organ scale, an imaging frontier that is not accessible by the existing tissue-sectioning microscopic techniques due to intensive light scattering beyond the depth of a few hundred microns. Biomedical optoacoustics has also emerged in the recent decade as a powerful tool for high-resolution visualization of optical contrast, overcoming a variety of longstanding limitations imposed by light scattering in deep tissues. By detecting tiny sound vibrations, resulting from selective absorption of light at multiple wavelengths, multispectral optoacoustic tomography methods can now “hear color” in three dimensions, i.e., deliver volumetric spectrally enriched (color) images from deep living tissues at high spatial resolution and in real time. These new-found imaging abilities directly relate to preclinical screening applications in animal models and are foreseen to significantly impact clinical decision making as well
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References
Ale A, Schulz R, Sarantopoulos A, Ntziachristos V (2010) Imaging performance of a hybrid x-ray computed tomography-fluorescence molecular tomography system using priors. Med Phys 37:1976–1986
Bowen T (1982) Radiation-induced thermoacoustic soft-tissue imaging. IEEE Trans on Son Ultrason 29: 187–187
Buehler A, Herzog E, Razansky D, Ntziachristos V (2010) Video rate optoacoustic tomography of mouse kidney perfusion. Opt Lett 35:2475–2477
Contag CH, Bachmann MH (2002) Advances in in vivo bioluminescence imaging of gene expression. Annu Rev Biomed Eng 4:235–260
Cox BT, Arridge SR, Kostli KP, Beard PC (2006) Two-dimensional quantitative photoacoustic image reconstruction of absorption distributions in scattering media by use of a simple iterative method. Appl Opt 45:1866–1875
De La Zerda A et al (2008) Carbon nano-tubes as photoacoustic molecular imaging agents in living mice. Nat Nanotechnol 3:557–562
Denk W, Strickler JH, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248:73–76
Giepmans BNG, Adams SR, Ellisman MH, Tsien RY (2006) Review—The fluorescent toolbox for assessing protein location and function. Science 312:217–224
Helmchen F, Denk W (2005) Deep-tissue two-photon microscopy. Nat Methods 2:932–940
Hyde D, Schulz R, Brooks D, Miller E, Ntziachristos V (2009) Performance dependence of hybrid x-ray computed tomography/fluorescence molecular tomography on the optical forward problem. J Opt Soc Am A: 26:919–923
Li L, Zemp R, Lungu G, Stoica G, Wang L (2007) Photoacoustic imaging of lacZ gene expression in vivo. JBO Lett vol. 12
Li ML, Oh JT, Xie XY, Ku G, Wang W, Li C, Lungu G, Stoica G, Wang LV (2008) Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography. Proc IEEE 96:481–489
Ntziachristos V (2006) Fluorescence Molecular Imaging. Annu Rev Biomed Eng 8:1–33
Ntziachristos V, Weissleder R (2001) Experimental three-dimensional fluorescence reconstruction of diffuse media using a normalized Born approximation. Opt Lett 26:893–895
Ntziachristos V, Razansky D (2010) Molecular imaging by means of multi-spectral opto-acoustic tomography (MSOT). ACR Chemical Review 110:2783–2794
Ntziachristos V, Ripoll J, Wang LHV, Weissleder R (2005) Looking and listening to light: the evolution of whole-body photonic imaging. Nat Biotechnol 23:313–320
Oraevsky AA, Esenaliev RO, Tittel FK (1994) Laser based optoacoustic imaging in biological tissues. Proc. SPIE 2134A: 122–128
Rayavarapu R, Petersen W, Ungureanu C, Post J, van Leeuwen T, Manohar S (2007) Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques. Int J Biomed Imaging p. 29817
Razansky D, Vinegoni C, Ntziachristos V (2007) Multispectral photoacoustic imaging of fluorochromes in small animals. Opt Lett 32:2891–2893
Razansky D, Ntziachristos V (2010) Multi-spectral optoacoustic tomography. In Knaeblein J (ed) Modern Biopharmaceuticals vol. in press, Wiley
Razansky D, Distel M, Vinegoni C, Ma R, Perrimon N, Koester R, Ntziachristos V (2009a) Going deeper than microscopy with multi-spectral optoacoustic tomography of fluorescent proteins in vivo. Nat Photonics 3:412–417
Razansky D, Baeten J, Ntziachristos V (2009b) Sensitivity of molecular target detection by multispectral optoacoustic tomography (MSOT). Med Phys 36:939–945
Razansky D, Vinegoni C, Ntziachristos V (2009c) Imaging of mesoscopic-scale organisms using selective-plane optoacoustic tomography. Phys Med Biol 54:2769–2777
Rosencwaig A (1973) Photoacoustic spectroscopy of biological materials. Science 181:657–658
Rosenthal A, Razansky D, Ntziachristos V (2009) Quantitative optoacoustic signal extraction using sparse signal representation. IEEE Trans Med Imag. 28:1997–2006
Rosenthal A, Razansky D, Ntziachristos V (2010) Fast semi-analytical model-based acoustic inversion for quantitative optoacoustic tomography. IEEE Trans Med Imaging 29:1275–1285
Schulz R, Ale A, Sarantopoulos A, Freyer M, Soehngen E, Zientkowska M, Ntziachristos V (2010) Hybrid system for simultaneous fluorescence and X-ray computed tomography. IEEE Trans Med Imag. 29:465–473
Soubret A, Ripoll J, Ntziachristos V (2005) Accuracy of fluorescent tomography in presence of heterogeneities: study of the normalized Born ratio. IEEE Med Imag. 24:1369–1376
Tsien RY (2005) Building and breeding molecules to spy on cells and tumors. FEBS Lett 579:927–932
Wang X, Pang Y, Ku G, Xie X, Stoica G, Wang LH (2003) Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain. Nat Biotechnol 21:803–806
Webb RH (1999) Theoretical basis of confocal microscopy. Methods Enzymol 307:3–20
Weissleder R, Ntziachristos V (2003) Shedding light onto live molecular targets. Nat Med 9:123–128
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Ntziachristos, V., Razansky, D. (2013). Optical and Opto-Acoustic Imaging. In: Schober, O., Riemann, B. (eds) Molecular Imaging in Oncology. Recent Results in Cancer Research, vol 187. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-10853-2_4
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