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PCR und Real-Time PCR

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Molekularbiologische Methoden in der Lebensmittelanalytik

Zusammenfassung

Die vielfältigen Anwendungsmöglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am häufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Für diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet. Die PCR erlaubt einen hochsensitiven und spezifischen in-vitro-Nachweis von Desoxyribonukleinsäuren (DNA), da im Zuge der Reaktion Sequenzabschnitte gezielt vermehrt werden. Innerhalb weniger Stunden können aus einem einzigen Zielmolekül 1012 identische Moleküle entstehen [1].

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Authors and Affiliations

  1. Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Veterinärstraße 2, 85764, Oberschleißheim, Deutschland

    Regina Konrad

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  1. Regina Konrad
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  2. Ulrich Busch
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Correspondence to Regina Konrad .

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Editors and Affiliations

  1. Gesundheitswesen Südbayern, Landesuntersuchungsamt für das, Veterinärstr. 2, Oberschleißheim, 85764, Germany

    Ulrich Busch

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Konrad, R., Busch, U. (2010). PCR und Real-Time PCR. In: Busch, U. (eds) Molekularbiologische Methoden in der Lebensmittelanalytik. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-10716-0_4

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