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QTL Analysis of Ascochyta Blight Resistance in Chickpea

  • Conference paper
Advances in Communication and Networking (FGCN 2008)

Part of the book series: Communications in Computer and Information Science ((CCIS,volume 27))

Abstract

Ascochyta blight, caused by Ascochyta rabiei(Pass.) Lab. is a devastating disease of chickpea (Cicer arietinum L.) worldwide. Resistant germplasm has been identified and the genetics of resistance has been the subject of numerous studies. Besides, microsatellites have become markers of choice for molecular mapping and marker assisted selection of key traits such as disease resistance in many crop species. The aim of this study was to construct a genetic linkage map and analysis of quantitative trait loci (QTLs) for Ascochyta blight resistance in chickpea (Cicer arietinum L.), as well as quantify the contribution of each QTL to observed phenotypic variation. Fifty-eight SSR markers and one morphological marker (flower color) were mapped on F2 individuals and F2:3 families derived from the cross ICC 12004 (resistant) (Bivanij (susceptible local variety) at the International Center for Agricultural Research at Dry Area (ICARDA). The linkage map comprised eight linkage groups, excluding flower color which didn’t assign to any linkage group. Area under disease progress curve (AUDPC) was used to evaluate the F2 population and F3 families. Using composite interval mapping, three genomic regions were detected, which were in association with reaction to ascochyta blight. These QTLs on LG3, LG4 and LG6 accounted for 46.5% of the total estimated phenotypic variation for reaction to ascochyta blight. Fine mapping of the QTLs identified in this study would lead to the identification of markers that could be used for marker-assisted selection of chickpea genotypes with resistance to Ascochyta blight. These findings are particular pertinent considering that we used Ascochyta rabiei pathotype III and ICC 12004 (resistant to pathotype III) for the first time.

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References

  1. Buchwaldt, L., Booker, H., Gali, K.: A detached leaf assay for phenotyping of chickpea-ascochyta interaction. In: Agriculture and Agri-Food Canada, Saskatoon, Saskatchewan, S7N0X2 (2007)

    Google Scholar 

  2. Chen, W., Muehlbauer, F.J.: An Improved Technique for virulence assay of Ascochyta rabiei on chickpea. International chickpea and pigeonpea Newsletter 10(2003), 31–33 (2003)

    Google Scholar 

  3. Chongo, G., Gossen, B.D., Buchwaldt, L., Adhikari, T., Rimmer, S.R.: Genetic diversity of Ascochyta rabiei in Canada. Plant Disease 88(1), 4–10 (2004)

    Article  Google Scholar 

  4. Collard, B.C.Y., Pang, E.C.K., Ades, P.K., Taylor, P.W.J.: Preliminary investigation of QTLs associated with seedling resistance to ascochyta blight from Cicer echinospermum, a wild relative of chickpea. Theor. Appl. Genet. 107, 719–729 (2003)

    Article  Google Scholar 

  5. Lincoln, S.E., Daly, M.J., Lander, E.S.: MAPMAKER/EXP version 3.0: A Tutorial and Reference Manual, 3rd edn. Whitehead Institute for Biomedical Research, Cambridge (1993)

    Google Scholar 

  6. Kaiser, W.J., Muehlbauer, F.J.: Occurrence of Ascochyta rabiei on imported chickpeas in eastern Washington. Phytopathology 74, 1139 (1984)

    Google Scholar 

  7. Nene, Y.L.: A review of Ascochyta blight of chickpea (Cicer arietinum L.). In: Saxena, M.C., Singh, K.B. (eds.) Ascochyta blight and winter sowing of chickpea, pp. 17–34. Martinus Nijhoff/Dr. W. Junk Publisher, The Hague, the Netherlands (1984)

    Google Scholar 

  8. Reddy, M.V., Kabbabeh, S.: Pathogenic variability in Ascochyta rabiei (Pass.) Lab. In: Syria, Lebanon (eds.) Phytopathology Mediterranean, vol. 24, pp. 265–266 (1984); Reddy, M.V., Singh, K.B.: Evaluation of a world collection of chickpea germplasm accessions for resistance to Ascochyta blight. Plant Dis. 68, 900–901 (1985)

    Article  Google Scholar 

  9. Santra, D.K., Tekeoglu, M., Ratnaparkhe, M., Kaiser, W.J., Muehlbauer, F.J.: Identification and mapping of QTLs conferring resistance to Ascochyta blight in chickpea. Crop. Sci. 40, 1606–1612 (2000)

    Google Scholar 

  10. SAS Institute Inc.: SAS/STAT user’s guide. Version 9.12. SAS Institute, Cary, North Carolina (2000)

    Google Scholar 

  11. Sethy, N.K., Shokeen, B., Bhatia, S.: Isolation and characterization of sequence-tagged microsatellite site markers in chickpea (Cicer arietinum L.). Mol. Ecol. Notes 3, 428–430 (2003)

    Article  Google Scholar 

  12. Singh, G.: Identification and designation of physiologic races of Ascochyta rabiei in India. Indian Phytopathology 43, 48–52 (1990)

    Google Scholar 

  13. Singh, K.B., Reddy, M.V.: Advances in disease resistance breeding in chickpea. Advanced Agronomy 45, 191–222 (1991)

    Article  Google Scholar 

  14. Singh, K.B., Reddy, M.V.: Resistance to Six Races of Ascochyta rabiei in the world germplasm collection of chickpea. Crop. Science 33, 186–189 (1993a)

    Google Scholar 

  15. Singh, K.B., Reddy, M.V.: Sources of resistance to ascochyta blight in wild Cicer species. Netherlands Journal of Plant Pathology 99, 163–167 (1993b)

    Article  Google Scholar 

  16. Singh, K.B., Malhotra, R.S., Halila, M.H., Knights, E.J., Verma, M.M.: Current status and future strategy in breeding chickpea for resistance to biotic and abiotic stresses. Euphytica. 73, 137–149 (1994)

    Article  Google Scholar 

  17. Singh, K.B., Reddy, M.V., Haware, M.: Breeding for resistance to ascochyta blight in chickpea. In: Singh, K.B., Saxena, M.C. (eds.) Disease resistance breeding in chickpea, ICARDA, Aleppo, Syria, pp. 23–54 (1992)

    Google Scholar 

  18. Tar’an, B., Warkentin, T.D., Tullu, A., Vandenberg, A.: Genetic mapping of ascochyta blight resistance in chickpea (Cicer arietinum L.) using a simple sequence repeat linkage map. Genome 50, 26–34 (2007)

    Article  Google Scholar 

  19. Tekeoglu, M., Santra, D.K., Kaiser, W.J., Muehlbauer, F.J.: Ascochyta blight resistance in three chickpea recombinant inbred line populations. Crop. Sci. 40, 1251–1256 (2000)

    Article  Google Scholar 

  20. Trapero-Casas, A., Kaiser, W.J.: Development of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea straw. Phytopathology 82, 1261–1266 (1992a)

    Article  Google Scholar 

  21. Trapero-Casas, A., Kaiser, W.J.: Influence of temperature, wetness period, plant age, and inoculum concentration on infection and development of ascochyta blight of chickpea. Phytopathology 82, 589–596 (1992b)

    Article  Google Scholar 

  22. Udupa, S.M., Baum, M.: Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theor. Appl. Genet. 106, 1196–1202 (2003)

    Google Scholar 

  23. Vir, S., Grewal, J.S.: Physiological specialization in Ascochyta rabiei, the causal organism of gram blight. Indian Phytopathology 27, 265–266 (1974)

    Google Scholar 

  24. Wang, S., Basten, C.J., Zeng, Z.B.: Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University. Raleigh N.C (2004), http://statgen.ncsu.edu/qtlcart/WQTLCart.htm

  25. Udupa, S.M., Weigand, F., Saxena, M.C., Kahl, G.: Genotyping with RAPD and microsatellite markers resolves pathotype diversity in the ascochyta blight pathogen of chickpea. Theor. Appl. Genet. 97, 299–307 (1998)

    Article  Google Scholar 

  26. Weising, K., Winter, P., Huttel, B., Kahl, G.: Microsatellite markers for molecular breeding. J. Crop. Prod. 1, 113–143 (1998)

    Article  Google Scholar 

  27. Wilson, A., Kaiser, W.: Cytology and genetics of sexual incompatibility in Didymella rabiei. Mycolgia 87, 795–804 (1995)

    Article  Google Scholar 

  28. Winter, P., Pfaff, T., Udupa, S.M., Hüttel, B., Sharma, P.C., Sahi, S., Arreguin-Espinoza, R., Weigand, F., Muehlbauer, F.J., Kahl, G.: Characterization and mapping of sequence-tagged Microsatellite sites in the chickpea (Cicer arietinum L.) Genome. Mol. Gen. Genet (1999)

    Google Scholar 

  29. Winter, P., Benko-Iseppon, A.M., Huttel, B., Ratnaparkhe, M., Tullu, A., Sonnante, G., Pfaff, T., Tekeoglu, M., Santra, D., Sant, V.J., Rajesh, P.N., Kahl, G., Muehlbauer, F.J.: A linkage map of the chickpea (Cicer arietinum L.) Genome based on the recombinant inbred lines from a C. arietinum xC. reticulatum cross: localization of resistance genes for Fusarium races 4 and 5. Theor. Appl. Genet. 101, 1155–1163 (2000)

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Professor in the Department of Agronomy & Plant breeding, Faculty of Crop & Animal Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, P.O. Box 31587-11167, Iran

    A. Taleei

  2. Seed and Plant Improvement Institute (SPII), 4119-31585, Karaj, Iran

    H. Kanouni

  3. International Centre for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria

    M. Baum

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  1. A. Taleei
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  2. H. Kanouni
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  3. M. Baum
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Editor information

Editors and Affiliations

  1. Hannam University, 133 Ojeong-dong, DaedeokGu, 306-791, Daejeon, South Korea

    Tai-hoon Kim

  2. Department of Computer Science, St. Francis Xavier University, Antigonish, NS, Canada

    Laurence T. Yang

  3. Department of Computer Science and Engineering, Kyungnam University, Masan, Kyungnam, South Korea

    Jong Hyuk Park

  4. National Chung Cheng University, Chiayi County, Taiwan

    Alan Chin-Chen Chang

  5. University of Western Macedonia, West Macedonia, Greece

    Thanos Vasilakos

  6. Division of Computer Engineering, Mokwon University, Daejeon, South Korea

    Sang-Soo Yeo

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© 2009 Springer-Verlag Berlin Heidelberg

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Taleei, A., Kanouni, H., Baum, M. (2009). QTL Analysis of Ascochyta Blight Resistance in Chickpea. In: Kim, Th., Yang, L.T., Park, J.H., Chang, A.CC., Vasilakos, T., Yeo, SS. (eds) Advances in Communication and Networking. FGCN 2008. Communications in Computer and Information Science, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-10236-3_3

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  • DOI: https://doi.org/10.1007/978-3-642-10236-3_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-10235-6

  • Online ISBN: 978-3-642-10236-3

  • eBook Packages: Computer ScienceComputer Science (R0)

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