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Molecular Characterization and Diagnosis of Macrophomina phaseolina: A Charcoal Rot Fungus

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Molecular Identification of Fungi

Abstract

Macrophomina phaseolina is a global pathogen that inflicts losses on many agriculturally important crops worldwide, particularly in warm and tropical environments. Efforts to divide M. phaseolina into subspecies have been unsuccessful largely due to the extreme intraspecific variations in morphology and pathogenecity. The failure to adequately identify and detect M. phaseolina using conventional culture-based morphological techniques has led to the development of nucleic acid-based molecular approaches. PCR-based methods are highly sensitive and specific and have the potential to replace traditional technologies. Recently, species-specific oligonucleotide primers and digoxigenin (DIG)-labeled probe were designed at internal transcribed spacer (ITS) region for identification and detection of M. phaseolina. Accurate diagnosis and early detection of pathogens is an essential step in agriculture and environmental monitoring including plant disease management. The main objective of this review is to outline various molecular tools used for detection, identification, and characterization of M. phaseolina isolates. We also emphasize the significance of advanced technique such as real-time polymerase chain reaction (PCR) for qualitative and quantitative assays.

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Acknowledgment

The work is supported by a Network Project, sponsored by Indian Council of Agricultural Research (ICAR), Government of India, New Delhi. The authors are thankful to the funding agency.

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Authors and Affiliations

  1. Environmental Microbiology Lab, Department of Environmental Engineering, Chosun University, Gwang, ju-501759, South Korea

    Bandamaravuri Kishore Babu

  2. National Bureau of Agriculturally Important Microorganisms (ICAR), Mau, Uttar Pradesh, 275101, India

    Bandamaravuri Kishore Babu & Dilip K. Arora

  3. Biotechnology Division, North-East Institute of Science & Technology (CSIR), Jorhat, 785006, Assam, India

    Ratul Saikia

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  1. Fac. Science, Dept. Botany, South Valley University, Qena, Egypt

    Youssuf Gherbawy

  2. School of Biology and Pharmacy, Institute of Microbiology, University of Jena, Neugasse 24, Jena, 07743, Germany

    Kerstin Voigt

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Babu, B.K., Saikia, R., Arora, D.K. (2010). Molecular Characterization and Diagnosis of Macrophomina phaseolina: A Charcoal Rot Fungus. In: Gherbawy, Y., Voigt, K. (eds) Molecular Identification of Fungi. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-05042-8_9

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