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Molecular Chemotyping of Fusarium graminearum, F. culmorum, and F. cerealis Isolates From Finland and Russia

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Molecular Identification of Fungi

Abstract

PCR assays that yield markers that are predictive of NIV versus DON production or 3ADON versus 15ADON production were performed for 60 Fusarium graminearum isolates, most of which were from Finland and Russia. None of the F. graminearum isolates originating from Finland and north-western Russia produced any PCR fragment with DON and NIV specific primers, which indicates that they have a 3ADON chemotype. All F. graminearum isolates from southern Russia and most of the isolates from Asia and Germany produced fragments typical of the 15ADON chemotype. All F. culmorum isolates belonged to 3ADON chemotype, while all F. cerealis isolates belonged to NIV chemotype. The highest DON levels were found in the 3ADON molecular chemotype isolates of Finland, north-western Russia, and central Russia. In the combined 3ADON molecular chemotype isolates, DON production was clearly higher than in the combined 15DON chemotype isolates.

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Acknowledgments

We are indebted to Drs. A. Burkin and G. Kononenko from Laboratory of Mycotoxicology, All-Russian Institute for Veterinary Sanitation, Hygiene, and Ecology, Moscow, Russia for performing mycotoxins analyses. The visits of Dr. T. Yli-Mattila to the All-Russian Plant Protection Institute and the visits of Dr. T. Gagkaeva to the University of Turku were supported financially by the Academy of Finland (no. 126917 and 131957) and the Nordic network project New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe (project 090014), which is funded by the Nordic Research Board. The authors are grateful to Drs. K. O’Donnell and T. Ward from USDA Research Institute in Peoria in USA for comments on the previous version of the manuscript and to Dr. Robert Proctor from the same institute for comments on the final version and for linguistic revision.

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Authors and Affiliations

  1. Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, 20014, Turku, Finland

    Tapani Yli-Mattila

  2. Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), 196608, St. Petersburg-Pushkin, Russia

    Tatiana Gagkaeva

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  1. Tapani Yli-Mattila
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Correspondence to Tapani Yli-Mattila .

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  1. Fac. Science, Dept. Botany, South Valley University, Qena, Egypt

    Youssuf Gherbawy

  2. School of Biology and Pharmacy, Institute of Microbiology, University of Jena, Neugasse 24, Jena, 07743, Germany

    Kerstin Voigt

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Yli-Mattila, T., Gagkaeva, T. (2010). Molecular Chemotyping of Fusarium graminearum, F. culmorum, and F. cerealis Isolates From Finland and Russia. In: Gherbawy, Y., Voigt, K. (eds) Molecular Identification of Fungi. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-05042-8_8

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