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Biosynthesis and Biotransformation

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Transgenic Crop Plants

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Abstract

Plant secondary metabolites, the so-called natural products, are used as flavors, food additives, and pharmaceuticals. Since complex natural products are not economically produced by total chemical synthesis except for some small molecules, most of the useful metabolites are still obtained from wild or cultivated plant resources. Alternatively, plant cell culture technique would be a potential method to produce these useful secondary metabolites, and commercial production of some useful secondary metabolites has been achieved (Kolewe et al. 2008; Smetanska 2008). Furthermore, biotransformation by plant cell cultures could be a useful method to convert natural products or unnatural synthetic products into chemically different products of economical importance. This chapter will focus on biosynthesis and biotransformation of useful chemical compounds using plant cell culture technology.

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References

  • Akiu S, Suzuki Y, Fujinuma Y, Asahara T, Fukuda M (1988) Inhibitory effect of arbutin in melanogenesis: biochemical study in cultured B16 melanoma cells and effect on the UV-induced pigmentation in human skin. Proc Jpn Soc Investig Dermatol 12:138–139

    Google Scholar 

  • Alfermann AW, Schuller I, Reinhard E (1980) Biotransformation of cardiac glycosides by immobilized cells of digitalis lanata. Planta Med 40:218–223

    Article  CAS  Google Scholar 

  • Bley KR (2004) Recent developments in transient receptor potential vanilloid receptor agonist-based therapies. Expert Opin Investig Drugs 13:1445–1456

    Article  CAS  PubMed  Google Scholar 

  • Botta B, Monache GD, Misiti D, Vitali A, Zappia G (2001) Aryltetralin ligrans: chemistry, pharmacology and biotransformations. Curr Med Chem 8:1363–1381

    CAS  PubMed  Google Scholar 

  • Eilert U, DeLuca V, Kurz WGW, Constabel F (1987) Alkaloid formation by habituated and tumorous cell suspension cultures of Catharanthus roseus. Plant Cell Rep 6:271–274

    Article  CAS  Google Scholar 

  • Eilert U, Kurz WGW, Constabel F (1985) Stimulation of sanguinarine accumulation in Papaver somniferum cell cultures by fungal elicitors. J Plant Physiol 119:65–76

    CAS  Google Scholar 

  • Facchini PJ, Hagel JM, Liscombe DK, Loukania N, MacLeod BP, Samanani N, Zulak KG (2007) Opium poppy: blueprint for an alkaloid factory. Phytochem Rev 6:97–124

    Article  CAS  Google Scholar 

  • Facchini PJ, De Luca V (2008) Opium poppy and madagascar periwinkle: model non-model systems to investigate alkaloid biosynthesis in plants. Plant J 54:763–784

    Article  CAS  PubMed  Google Scholar 

  • Frense D (2007) Taxanes: perspectives for biotechnological production. Appl Microbiol Biotech-nol 73:1233–1240

    Article  CAS  Google Scholar 

  • Fujita Y, Hara Y, Suga C, Morimoto T (1981) Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon. II. A new medium for the production of shikonin derivatives. Plant Cell Rep 1:61–63

    Article  CAS  Google Scholar 

  • Furuya T, Kawaguchi K, Hirotani M (1988) Biotransformation of digitoxigenin by cell suspension cultures of Strophanthus gratus. Phytochemistry 27:2129–2133

    Article  CAS  Google Scholar 

  • Furuya T, Yoshikawa T, Orihara Y, Oda H (1983) Saponin production in cell suspension cultures of Panax ginseng. Planta Med 48:83–87

    Article  CAS  PubMed  Google Scholar 

  • Georgiev MI, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74:1175–1185

    Article  CAS  PubMed  Google Scholar 

  • Hashimoto T, Hayashi A, Amano Y, Kohno J, Iwanari H, Usuda S, Yamada Y (1991) Hyoscya-mine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. J Biol Chem 266:4648–4653

    CAS  PubMed  Google Scholar 

  • Hashimoto T, Yamada Y (1994) Alkaloid biogenesis: molecular aspects. Annu Rev Plant Physiol Plant Mol Biol 45:257–285

    CAS  Google Scholar 

  • Hayashi H, Fukui H, Tabata M (1988) Examination of triterpenoids produced by callus and cell suspension cultures of Glycyrrhiza glabra. Plant Cell Rep 7:508–511

    Article  CAS  Google Scholar 

  • Hayashi H, Sakai T, Fukui H, Tabata M (1990) Formation of soyasaponins in licorice cell suspension cultures. Phytochemistry 29:3127–3129

    Article  CAS  Google Scholar 

  • Hayashi H, Fukui H, Tabata M (1993) Distribution pattern of saponins in different organs of Glycyrrhiza glabra. Planta Med 59:351–353

    Article  CAS  PubMed  Google Scholar 

  • Hayashi H, Huang P, Inoue K (2003) Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra. Plant Cell Physiol 44:404–411

    Article  CAS  PubMed  Google Scholar 

  • Hayashi H, Huang P, Takada S, Obinata M, Inoue K, Shibuya M, Ebizuka Y (2004) Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra. Biol Pharm Bull 27:1086–1092

    Article  CAS  PubMed  Google Scholar 

  • Heide L, Nishioka N, Fukui H, Tabata M (1989) Enzymatic regulation of shikonin biosynthesis in Lithospermum erythrorhizon cell cultures. Phytochemistry 28:1873–1877

    Article  CAS  Google Scholar 

  • Hirotani M, Furuya T (1980) Biotransformation of digitoxigenin by cell suspension cultures of Digitalis purpurea. Phytochemistry 19:531–534

    Article  CAS  Google Scholar 

  • Inomata S, Yokoyama M, Seto S, Yanagi M (1991) High level production of arbutin from hydroquinone in suspension cultures of Catharanthus roseus. Plant Cell Physiol 36:315–319

    CAS  Google Scholar 

  • Jennewein S, Croteau R (2001) Taxol: biosynthesis, molecular genetics, and biotechnological applications. Appl Microbiol Biotechnol 57:13–19

    Article  CAS  PubMed  Google Scholar 

  • Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep 5:239–242

    Article  CAS  Google Scholar 

  • Kaminaga Y, Nagatsu A, Akiyama T, Sugimoto N, Yamazaki T, Maitani T, Mizukami H (2003) Production of unnatural glucosides of curcumin with drastically enhanced water solubility by cell suspension cultures of Catharanthus roseus. FEBS Lett 555:311–316

    Article  CAS  PubMed  Google Scholar 

  • Kawaguchi K, Hirotani M, Furuya T (1989) Biotransformation of digitoxigenin by cell suspension cultures of Strophanthus intermedius. Phytochemistry 28:1093–1097

    Article  CAS  Google Scholar 

  • Kim YS, Cho JH, Ahn J, Hwang B (2006) Upregulation of isoprenoid pathway genes during enhanced saikosaponin biosynthesis in the hairy roots of Bupleurum falcatum. Mol Cell 22:269–274

    Article  CAS  Google Scholar 

  • Kitajima M, Fischer U, Nakamura M, Ohsawa M, Ueno M, Takayama H, Unger M, Stoeckigt J, Aimi N (1998) Anthraquinones from Ophiorrhiza pumila tissue and cell cultures. Phytochem-istry 48:107–111

    Article  CAS  Google Scholar 

  • Kolewe ME, Gaurav V, Roberts SC (2008) Pharmaceutically active natural product synthesis and supply via plant cell culture technology. Mol Pharm 5:243–256

    Article  CAS  PubMed  Google Scholar 

  • Kometani T, Tanimoto H, Nishijima T, Kanabara I, Okada S (1993) Glucosylation of capsaicin by cell suspension cultures of Coffea arabica. Biosci Biotechnol Biochem 57:2192–2193

    Article  CAS  Google Scholar 

  • Kopec SE, Irwin RS, DeBellis RJ, Bohlke MB, Maher TJ (2008) The effects of Tween-80 on the integrity of solutions of capsaicin: useful information for performing tussigenic challenges. Cough 4:3

    Article  PubMed  Google Scholar 

  • Kutney JP, Chen YP, Gao S, Hewitt GM, Kuri-Brena F, Milanova RK, Stoynov N (1993) Studies with plant cell cultures of Podophyllum peltatum L. II. Biotransformation of dibenzylbutano-lides to lignans. Development of a “biological factory” for lignan synthesis. Heterocycles 36:13–20

    Article  CAS  Google Scholar 

  • Li SM, Hennig S, Heide L (1998) Shikonin: a geranyl diphosphate-derived plant hemiterpenoid formed via the mevalonate pathway. Tetrahedron Lett 39:2721–2724

    Article  CAS  Google Scholar 

  • Liu C, Zhao Y, Wang Y (2006) Artemisinin: current state and perspectives for biotechnological production of an antimalarial drug. Appl Microbiol Biotechnol 72:11–20

    Article  CAS  PubMed  Google Scholar 

  • Lorence A, Medina-Boliver F, Nessler CL (2004) Camptothecin and 10-hydroxycamptothecin from Camptotheca acuminata hairy root. Plant Cell Rep 22:437–441

    Article  CAS  PubMed  Google Scholar 

  • Lorence A, Nessler CL (2004) Camptothecin, over four decades of surprising findings. Phyto-chemistry 65:2735–2749

    CAS  Google Scholar 

  • Maheshwari RK, Singh AK, Gaddipati J, Srimal RC (2006) Multiple biological activities of curcumin: a short review. Life Sci 27:2081–2087

    Article  Google Scholar 

  • Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures. Agric Biol Chem 50:2715–2722

    CAS  Google Scholar 

  • Mano Y, Ohkawa H, Yamada Y (1989) Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes. Plant Sci 59:191–201

    Article  CAS  Google Scholar 

  • Masada S, Kawase Y, Nagatoshi M, Oguchi Y, Terasaka K, Mizukami H (2007) An efficient chemoenzymatic production of small molecule glucosides with in situ UDP-glucose recycling. FEBS Lett 581:2562–2567

    Article  CAS  PubMed  Google Scholar 

  • Matsuda J, Okabe S, Hashimoto T, Yamada Y (1991) Molecular cloning of hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger. J Biol Chem 266:9460–9464

    CAS  PubMed  Google Scholar 

  • Mihara S, Hiraoka K, Kameda W (1992) Synthesis of N-[4-(R-D-glucopyranosyloxy)-3-methox-ybenzyl] nonamide and its β-anomer. J Agric Food Chem 40:2057–2059

    Article  CAS  Google Scholar 

  • Mizukami H, Konoshima M, Tabata M (1978) Variation in pigment production in Lithospermum erythrorhizon callus cultures. Phytochemistry 17:95–97

    Article  CAS  Google Scholar 

  • Moyano E, Palazón J, Bonfill M, Osuna L, Cusinó RM, Oksman-Caldentey KM, Piñol MT (2007) Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures. J Plant Physiol 164:521–524

    Article  CAS  PubMed  Google Scholar 

  • Nakagawa K, Konagai A, Fukui H, Tabata M (1984) Release and crystallization of berber-ine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep 3:254–257

    Article  CAS  Google Scholar 

  • Orihara Y, Miyatake H, Furuya T (1991) Triglucosylation on the biotransformation of (+)-menthol by cultured cells of Eucaryptus perriniana. Phytochemistry 30:1843–1845

    Article  CAS  PubMed  Google Scholar 

  • Paniego NB, Giuletti AM (1994) Artemisia annua L. dedifferentiated and differentiated cultures. Artemisinin production. Plant Cell Tissue Organ Cult 36:163–168

    Article  CAS  Google Scholar 

  • Papageorgiou VP, Assimopoulou AN, Couladouros EA, Hepworth D, Nicolaou KC (1999) The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angew Chem Int Ed 38:270–300

    Article  Google Scholar 

  • Park SU, Facchini PJ (2000) Agrobacterium rhizogenes—mediated transformation of opium poppy, Papaver somniferum L., and California poppy, Eschscholzia californica Cham., root cultures. J Exp Bot 347:1005–1016

    Article  Google Scholar 

  • Parr AJ, Peerless ACJ, Hamill JD, Walton NJ, Robins RJ, Rhodes MJC (1988) Alkaloid production by transformed root cultures of Catharanthus roseus. Plant Cell Rep 7:309–312

    Article  CAS  Google Scholar 

  • Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Production of hyoscyamine by hairy root cultures of Datura stramonium. Planta Med 53:474–478

    Article  CAS  PubMed  Google Scholar 

  • Puricelli L, Caniato R, Monache GD (2003) Biotransformation of a dibenzylbutanolide to podo-phyllotoxin analogues by shoot cultures of Haplophyllum patavinium. Chem Pharm Bull 51:848–850

    Article  CAS  PubMed  Google Scholar 

  • Rohmer M (1999) The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae, and higher plants. Nat Prod Rep 16:565–574

    Article  CAS  PubMed  Google Scholar 

  • Rao RS, Ravishankar GA (1999) Biotransformation of isoeugenol to vanilla flavour metabolites and capsaicin in freely suspended and immobilized cell cultures of Capsicum frutescens: study of the influence of β-cyclodextrin and fungal elicitor. Process Biochem 35:341–348

    Article  CAS  Google Scholar 

  • Saito K, Sudo H, Yamazaki M, Koseki-Nakamura M, Kitajima M, Takayama H, Aimi N (2001) Feasible production of camptothecin by hairy root culture of Ophiorrhiza pumila. Plant Cell Rep 20:267–271

    Article  CAS  Google Scholar 

  • Sakato K, Tanaka H, Mukai N, Misawa M (1974) Isolation and identification of camptothecin from cells of Camptotheca acuminata suspension cultures. Agric Biol Chem 38:217–218

    CAS  Google Scholar 

  • Sato F, Yamada Y (1984) High berberine producing cultured Coptis japonica cells. Phytochemis-try 23:281–285

    Article  CAS  Google Scholar 

  • Seki H, Ohyama K, Sawai S, Mizutani M, Ohnishi T, Sudo H, Akashi T, Aoki T, Saito K, Muranaka T (2008) Licorice β-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin. Proc Natl Acad Sci USA 105:14204–14209

    Article  CAS  PubMed  Google Scholar 

  • Shibata S (2000) A drug over the millennia: pharmacognosy, chemistry, and pharmacology of licorice. Yakugaku Zasshi 120:849–862

    CAS  PubMed  Google Scholar 

  • Shimoda K, Kondo Y, Nishida T, Hamada H, Nakajima N, Hamada H (2006) Biotransformation of thymol, carvacrol, and eugenol by cultured cells of Eucalyptus perriniana. Phytochemistry 67:2256–2261

    Article  CAS  PubMed  Google Scholar 

  • Shimoda K, Kwon S, Utsuki A, Ohiwa S, Katsuragi H, Yonemoto N, Hamada H, Hamada H (2007) Glycosylation of capsaicin and 8-nordihydrocapsaicin by cultured cells of Cathar-anthus roseus. Phytochemistry 68:1391–1396

    Article  CAS  PubMed  Google Scholar 

  • Smetanska I (2008) Production of secondary metabolites using plant cell cultures. Adv Biochem Eng Biotechnol 111:187–228

    CAS  PubMed  Google Scholar 

  • Spieler H, Alfermann AW, Reinhard E (1985) Biotransformation of b-methyldigitoxin by cell cultures of Digitalis lanata in airlift and stirred tank reactors. Appl Microbiol Miotechnol 23:1–4

    Article  CAS  Google Scholar 

  • Suga T, Hirata T (1990) Biotransformation of exogeneous substrates by plant cell cultures. Phytochemistry 29:2393–2406

    Article  CAS  Google Scholar 

  • Tabata M, Yamamoto H, Hiraoka N, Konoshima M (1972) Organization and alkaloid production in tissue cultures of Scopolia parviflora. Phytochemistry 11:949–955

    Article  CAS  Google Scholar 

  • Tabata M, Mizukami H, Hiraoka N, Konoshima M (1974) Pigment formation of callus cultures of in Lithospermum erythrorhizon. Phytochemistry 13:927–932

    Article  CAS  Google Scholar 

  • Tabata M, Ikeda F, Hiraoka N, Konoshima M (1976) Glucosylation of phenolic compounds by Datura innoxia cell suspension cultures. Phytochemistry 15:1225–1229

    Article  CAS  Google Scholar 

  • Tabata M, Fujita Y (1985) Production of shikonin by plant cell cultures. In: Zaitlin M, Day P, Hollander A (eds) Biotechnology in Plant Science. Acad Press, Orland, pp 207–218

    Google Scholar 

  • Tabata M (1996) The mechanism of shikonin biosynthesis in Lithospermum cell cultures. Plant Tissue Cult Lett 13:117–125

    CAS  Google Scholar 

  • Tani M, Takeda K, Yazaki K, Tabata M (1993) Effects of oligogalacturonides on biosynthesis of shikonin in Lithospermum cell cultures. Phytochemistry 34:1285–1290

    Article  CAS  Google Scholar 

  • Tani Y, Fujioka T, Hamada H, Kunimatsu M, Furuichi Y (2003) Favorable effects of vanillylnonamide-b-D-glucoside on serum lipids in hyperlipidemic rats. J Jpn Soc Nutr Food Sci 56:181–187

    CAS  Google Scholar 

  • Toivonen L, Balsevich J, Kurz WGW (1989) Indole alkaloid production by hairy root cultures of Catharanthus roseus. Plant Cell Tissue Organ Cult 18:79–93

    Article  CAS  Google Scholar 

  • Toivonen L, Rosenqvit H (1995) Establishment and growth characteristics of Glycyrrhiza glabra hairy root cultures. Plant Cell Tissue Organ Cult 41:249–258

    Article  Google Scholar 

  • Uomori A, Seo S, Tomita Y (1974) Studies on the constituents in tissue cultures of Bupleurum falcatum L. Syoyakugaku Zasshi 28:152–160

    CAS  Google Scholar 

  • Waller GR, Nowacki EK (1978) Alkaloid Biology and Metabolism in Plant. Plenum Publ, New York, USA

    Google Scholar 

  • Weathers PJ, Cheetham RD, Follansbee E, Teoh K (1994) Artemisinin production by transformed roots of Artemisia annua. Biotechnol Lett 16:1281–1286

    CAS  Google Scholar 

  • Wildung MR, Croteau R (1996) A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis. J Biol Chem 271:9201–9204

    Article  CAS  PubMed  Google Scholar 

  • Yamada Y, Endo T (1984) Tropane alkaloid production in cultured cells of Duboisia leichhardtii. Plant Cell Rep 3:186–188

    Article  CAS  Google Scholar 

  • Yamada Y, Hashimoto T (1982) Production of tropane alkaloids in cultured cells of Hyoscyamus niger. Plant Cell Rep 1:101–103

    Article  CAS  Google Scholar 

  • Yamamoto H, Zhao P, Yazaki K, Inoue K (2002) Regulation of lithospermic acid B and shikonin production in Lithospermum erythrorhizon cell suspension cultures. Chem Pharm Bull 50:1086–1090

    Article  CAS  PubMed  Google Scholar 

  • Yamazaki Y, Sudo H, Yamazaki M, Aimi N, Saito K (2003) Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: Cloning, characterization and differential expression in tissues and by stress compounds. Plant Cell Physiol 44:395–403

    Article  CAS  PubMed  Google Scholar 

  • Yazaki K, Takeda K, Tabata M (1997) Effects of methyl jasmonate on shikonin and dihydroechi-nofuran production in Lithospermum cell cultures. Plant Cell Physiol 38:776–782

    CAS  Google Scholar 

  • Yazaki K, Kunihisa M, Fujisaki T, Sato F (2002) Geranyl diphosphate: 4-hydroxybenzoate geranyltransferase from Lithospermum erythrorhizon. Cloning and characterization of a key enzyme in shikonin biosynthesis. J Biol Chem 277:6240–6246

    Article  CAS  PubMed  Google Scholar 

  • Yamazaki Y, Kitajima M, Arita M, Takayama H, Sudo H, Yamazaki M, Aimi N, Saito K (2004) Biosynthesis of camptothecin. In silico and in vitro tracer study from [1-13C] glucose. Plant Physiol 134:161–170

    Article  CAS  PubMed  Google Scholar 

  • Yokoyama M, Inomata S, Seto S, Yanagi M (1990) Effect of sugars on the glucosylation of exogenous hydroquinone by Catharanthus roseus cells in suspension culture. Plant Cell Physiol 31:551–555

    CAS  Google Scholar 

  • Yukimune Y, Tabata H, Higashi Y, Hara Y (1996) Methyl jasmonate induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat Biotechnol 14:1129–1132

    Article  CAS  PubMed  Google Scholar 

  • Yun DJ, Hashimoto T, Yamada Y (1992) Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition. Proc Natl Acad Sci USA 89:11799–11803

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku Nagoya, 467-8603, Japan

    Hajiem Mizukami

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  1. Hajiem Mizukami
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  2. Hiroaki Hayashi
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Correspondence to Hajiem Mizukami .

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Editors and Affiliations

  1. Department of Genetics & Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Chittaranjan Kole

  2. NSF I/UCRC Center for Tree Genetics, Hardwood Tree Improvement and Regeneration Center at Purdue University, West Lafayette, IN, 47907, USA

    Charles H. Michler

  3. Department of Genetics & Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Albert G. Abbott

  4. Institute of Developmental & Molecular Biology Department of Biology, Texas A&M University, College Station, TX, 77843, USA

    Timothy C. Hall

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Mizukami, H., Hayashi, H. (2010). Biosynthesis and Biotransformation. In: Kole, C., Michler, C.H., Abbott, A.G., Hall, T.C. (eds) Transgenic Crop Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04809-8_9

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