Abstract
Humans have been manipulating the genetic information of plants throughout the history of agriculture. In this respect, every new plant variety or animal race is a result of the introduction of novel metabolic changes. This process has been slowly advancing for millennia. However, with the discovery of biochemical pathways and later with the introduction of gene manipulation techniques in 1970s, the pace greatly speeded up. Already in the mid-1980s, many of the compounds and enzymes participating in metabolic pathways were linked to their cloned genes, which can then be used for engineering the plant metabolism. Soon, novel products from plants appeared including, vaccines and other pharmaceuticals, plastics, and proteins that may render certain plants as effective tools for environmental decontamination. These products were a result of the manipulation of plant endogenous biochemical pathways and thus the novel field of science-metabolic engineering was born. Metabolic engineering can be defined as the targeted and purposeful modification of metabolic pathways in an organism for the improved use of cellular pathways for chemical transformation, energy transduction, and macromolecular synthesis or breakdown, potentially benefiting the society by producing biological substitutes for toxic chemicals, increasing agricultural production, improving industrial fermentation processes, producing completely new compounds, or by understanding the molecular mechanism underlying medical conditions in order to develop new cures (Kurnaz 2005).
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References
Abaddi A, Domergue F, Bauer J, Napier J, Welti R, Zahringer U, Cirpus P, Heinz E (2004) Biosynthesis of very long chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant cell 16:2734–2748
Aida R, Kishimoto S, Tanaka Y, Shibata M (2000) Modification of flower color in torenia (Torenia fournieri Lind.) by genetic transformation. Plant Sci 153:33–42
Akashi T, Fukuchi-Mizutani M, Aoki T, Ueyama Y, Yonekura-Sakakibara K, Tanaka Y, Kusumi T, Ayabe S (1999) Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones. Plant Cell Physiol 40:1182–1186
Alabadi D, Carbonell J (1998) Expression of ornithine decarboxylase is transiently increased bypollination, 2, 4-Dichlorophenoxyacetic acid and gibberellic acid in tomato ovaries. Plant Physiol 118:323–328
Anai T, Koga M, Tanaka H, Kinoshita T, Rahman S, Takagi Y (2003) Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene. Plant Cell Rep 21:988–992
Andersson-Gunneras S, Mellerowicz EJ, Love J, Segerman B, Ohmiya Y, Coutinho PM, Nillson P, Henrissat B, Moritz T, Sundberg B (2006) Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis. Plant J 45:144–165
Arai Y, Nakashita H, Doi Y, Yamaguchi I (2001) Plastid targeting of polyhydroxybutyrate biosynthetic pathway in tobacco. Plant Biotechnol 18:289–293
Arioli T, Peng L, Betzner A, Burn J, Wittke W, Herth W, Camillerie C, Hoffe H, Plazinski J, Birch R, Cork A, Glover J, Redmond J, Williamson R (1998) Molecular analysis of cellulose biosynthesis in Arabidopsis. Science 279:717–720
Bailey JE (1991) Toward a science of metabolic engineering. Science 252:1668–1675
Bailey JE, Sburlati A, Hatzimanikatis V, Lee K, Renner WA, Tsai PS (1996) Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. Biotechnol Bioeng 52:109–121
Barta A, Sommergruber K, Thomson D (1986) The expression of a nopaline synthase- human growth hormone chimeric gene in transformed tobacco and sunflower callus tissue. Plant Mol Biol 6:347–357
Bhattacharya E, Rajam M (2007) Polyamine biosynthetic pathway: a potential target for alkaloid production. In: Verpoorte R, Alfermann A, Johnson T (eds) Applications of plant metabolic engineering. Springer, Dordrecht, pp 129–143
Bock R, Khan MS (2004) Taming plastids for a green future. Trends Biotechnol 22:311–318
Bohm BA (1998) Introduction to Flavonoids, 1st edn. Taylor and Francis, London
Bordetsky A, Hwang R, Korin A, Lovaas D, Tonachel L (2005) Securing America: solving our oil dependence through innovation. Natural Resources Defense Council, New York
Bovy A, de Vos R, Kemper M, Schiljen E, Almenar Pertejo M, Muir S, Collins G, Robinson S, Verhoyen M, Hughes S (2002) High-flavanol tomatoes resulting from the heterologous expression of the maize transcription factor genes LC and C1. Plant cell 14:2509–2526
Broun P (2004) Transcription factors as tools for metabolic engineering in plants. Curr Opin Plant Biol 7:202–209
Broun P, Poindexter P, Osborne E, Jiang C-Z, Riechmann J (2004) WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis. Proc Natl Acad Sci USA 101:4706–4711
Burn J, Hocart C, Birch R, Cork A, Williamson R (2002) Functional analysis of the cellulose synthase genes CesA1, CesA2, and CesA3 in Arabidopsis. Plant Physiol 129:797–807
Cahoon E, Hall S, Ripp K, Ganzke T, Hitz W, Couglan S (2003) Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nat Bio-technol 21:1082–1087
Capell T, Christou T (2004) Progress in plant metabolic engineering. Curr Opin Biotechnol 15:148–154
Chattopadhyay M, Gupta S, Sengupta D (1997) Expression of arginine decarboxylase in seedlings of indica rice (Oryza sativa L.) cultivars as affected by salinity stress. Plant Mol Biol 34:477–483
Chen T, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opin Plant Biol 5:250–257
Cornwell T, Cohick W, Raskin I (2004) Dietary phytoestrogens and health. Phytochemistry 65:995–1016
Damude H, Kinney A (2007) Mertabolic engineering of seed oil biosynthetic pathways for human health. In: Verpoorte R, Alfermann A, Johnson T (eds) Applications of Plant Metabolic Engineering. Springer, Dordrecht, The Netherlands, pp 237–247
Damude H, Yadav N (2005) Cloning and sequences of fungal δ—15 desaturases suitable for production of polyunsaturated fatty acids in oilseed plants for food or industrial uses. In: PCT Int ApplWO2005047479
Damude H, Zhang H, Farrall L (2004) Identification of bifunctional δ12/ω3 fatty acid desaturases for improving the ratio of o3 to o6 fatty acids in microbes and plants. Proc Natl Acad Sci USA 103:9446–9451
Daniell H (2002) Molecular strategies for gene containment in transgenic crops. Nat Biotechnol 20:581–586
Daniell H, Guda C, McPherson DT, Zhang X, Xu J, Urry DW (1997) Hyperexpression of a synthetic protein-based polymer gene. Methods Mol Biol 63:359–371
Daniell H, Streatfield SJ, Wycoff K (2001a) Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 6:219–226
Daniell H, Wiebe PO, Millan AF (2001b) Antibiotic-free chloroplast genetic engineering — an environmentally friendly approach. Trends Plant Sci 6:237–239
Daniell H, Chebolu S, Kumar S, Singleton M, Falconer R (2005a) Chloroplast-derived vaccine antigens and other therapeutic proteins. Vaccine 23:1779–1783
Daniell H, Kumar S, Dufourmantel N (2005b) Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol 23:238–245
Datta K, Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan J, Rai M, Rehana S, A-B S, Beyer P, Potrykus I, Datta S (2003) Bioengineered golden Indica rice cultivars with beta-carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnol J 1:81–90
De Zoeten G, Penwick J, Horisberger M (1989) The expression, localization and effect of ahuman interferon in plants. Virology 172:213–222
Del Vecchio A (1996) High laurate canola. Inform 7:230–243
Delgado J, Liao JC (1997) Inverse flux analysis for reduction of acetate excretion in Escherichia coli. Biotechnol Prog 13:361–367
Dellapenna D (2007) Biofortification of plant-based food: enhancing folate levels by metabolic engineering. Proc Natl Acad Sci USA 104:3675–3676
Dewick PM (1994) The Flavonoids: Advances in Research since 1986. In: Harborne JB (ed) The Flavonoids: Advances in Research. Chapman and Hall, London, New York, pp 117–232
Diaz de la Garza R, Gregory J, Hanson A (2007) Folate biofortification of tomato fruit. Proc Natl Acad Sci USA 104:4218–4222
Dixon RA, Steele CL (1999) Flavonoids and isoflavonoids — a gold mine for metabolic engineering. Trends Plant Sci 4:394–400
Dixon RA, Sumner LW (2003) Legume natural products: understanding and manipulating complex pathways for human and animal health. Plant Physiol 131:878–885
Drexler H, Spiekermann P, Meyer A, Domergue F, Zank T, Sperling P, Abaddi A, Heinz E (2003) Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. J Plant Physiol 160:779–802
Dufourmantel N, Tissot G, Goutorbe F, Garcon F, Muhr C, Jansens S, Pelissier B, Peltier G, Dubald M (2005) Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin. Plant Mol Biol 58:659–668
During K (1988) Wound-inducible expression and secretion of T4 lysozyme and monoclonal antibodies in Nicotiana tabacum. PhD thesis. Mathematish-Naturwissenschaftlichen Fakultaet der Universitaet Zu Koeln
Ebel J, Schmidt WE, Loyal R (1986) Phytoalexin synthesis: the biochemical analysis of the induction process. Annu Rev Phytopathol 24:235–264
Ellestad G (2006) Structure and chiroptical properties of supramolecular flower pigments. Chirality 18:134–144
Elomaa P, Holton T (1994) Modification of flower color using genetic engineering. Biotechnol Genet Eng Rev 12:63–88
Emani C, Jiang Y, Miro B, Hall T, Kohli A (2008) Rice. In: Kole C, Hall T (eds) Compendium of transgenic crop plants, vol 1, Cereals and forage grasses. Wiley-Blackwell, Oxford, pp 1–47
Ferguson BJ, Mathesius U (2003) Signaling interactions during nodule development. J Plant Growth Reg 22:47–72
Fernandez-San Millan A, Mingo-Castel A, Miller M, Daniell H (2003) A chloroplast transgenic approach to hyper-express and purify Human Serum Albumin, a protein highly susceptible to proteolytic degradation. Plant Biotechnol J 1:71–79
Fischer R, Emans N (2000) Molecular farming of pharmaceutical proteins. Transgenic Res 9:279–299
Forkmann G (1991) Flavonoids as flower pigments: the formation of the natural spectrum and its extension by genetic engineering. Plant Breed 106:1–26
Forkmann G, Heller W, Grisebach H (1980) Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3′-hydroxylases. Z Naturforsch C 35:691–695
Fraley R, Rogers S, Horsch R (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80:4803–4807
Fujita N, Kubo A, Suh D, Wong K, Jane J, Ozawa K, Takaiwa F, Inaba Y, Nakamura Y (2003) Antisense inhibition of isoamylase alters the structure of amylopectin and the physicochemical properties of starch in rice endosperm. Plant Cell Physiol 44:607–618
Fukada-Tanaka S, Inagaki Y, Yamaguchi T, Saito N, Iida S (2000) Color-enhancing protein in blue petals. Nature 407:581
Fukui Y, Tanaka Y, Kusumi T, Iwashita T, Nomoto K (2003) A rationale for the shift in color towards blue in transgenic carnation flowers expressing the flavonoid 3′, 5′-hydroxylase gene. Phytochemistry 63:15–23
Galili G, Hofgen R (2002) Metabolic engineering of amino acids and storage proteins in plants. Metab Eng 4:3–11
Goff S, Klein T, Roth B, Fromm M, Cone K, Radicella J, Chandler V (1990) Transactivation of anthocyanin in biosynthetic genes following transfer of B regulatory genes into maize tissues. EMBO J 9:2517–2522
Goto T, Kondo T (1991) Structure and molecular stacking of anthocyanins — flower Color variation. Angew Chem 30:17–33
Graham TL, Graham MY (1996) Signaling in soybean phenylpropanoid responses (dissection of primary, secondary, and conditioning effects of light, wounding, and elicitor treatments). Plant Physiol 110:1123–1133
Grotewold E (2006) The genetics and biochemistry of floral pigments. Annu Rev Plant Biol 57:761–780
Hale K, McGrath S, Lombi E, Stack S, Terry N, Pickering I, George G, Pilon-Smits E (2001) Molybdenum sequestration in Brassica species. A role for anthocyanins? Plant Physiol 126:1391–1402
Han J, Luhs W, Sonntag K, Zahringer U, Borchardt D, Wolter F, Heinz E, Frentzen M (2001) Functional characterization of β-ketoacyl-CoA synthase genes from Brassica napus L. Plant Mol Biol 46:229–239
Haq T, Mason H, Clements J (1995) Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268:714–716
Harborne JB (ed) (1988) The Flavonoids: advances in research since 1980. Chapman and Hall, London
Harborne JB (ed) (1994) The Flavonoids: advances in research since 1980, 1st edn. Chapman and Hall, London
Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504
Hashimoto T, Tamaki K, Suzuki K (1998) Molecular cloning of plant spermidine synthases. Plant Cell Physiol 39:73–79
Hiatt A, Cafferkey R, Bowdish K (1989) Production of antibodies in transgenic plants. Nature 342:76–78
Holton TA, Tanaka Y (1994) Blue roses — a pigment of our imagination? Trends Biotechnol 12:40–42
Horsch RB, Fry JE, Hoffman NL, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
Jefferson R, Kavanaugh T, Bevan M (1987) GUS fusion: b-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Johnson ET, Yi H, Shin B, Oh BJ, Cheong H, Choi G (1999) Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins. Plant J 19:81–85
Jung W, Yu O, Lau SMC, O'Keefe DP, Odell J, Fader G, McGonigle B (2000) Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. Nat Biotechnol 18:208–212
Karunanandaa B, Qi Q, Hao M, Baszis S, Jensen P, Wong Y, Jiang J, Venkatramesh M, Gruys K, Moshiri F (2005) Metabolically engineered oilseed crops with enhanced seed tocopherol. Metab Eng 7:384–400
Katsumoto Y, Fukuchi-Mizutani M, Fukui Y, Brugliera F, Holton T, Mirko K, Noriko N, Yonekura-Sakakibara K, Togami J, Pigeaire A, Tao G, Nehra N, Lu CY, Dyson B, Tsuda S, Ashikari T, Kusumi T, Mason J, Tanaka Y (2007) Engineering of the rose flavanoid biosyn-thetic pathway succesfully generated blue-hued flowers accumulating delphinidin. Plant Cell Physiol 48:1589–1600
Kawagoe Y, Delmer D (1997) Pathways and genes involved in cellulose biosynthesis. Genet Eng 19:63–87
Kawaoka A, Kaothien P, Yoshida K, Endo S, Yamada K, Ebinuma H (2000) Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis. Plant J 22:289–301
Kinney A (2006) Metabolic engineering in plants for human health and nutrition. Curr Opin Biotechnol 17:130–138
Kinney A, Knowlton S, Blackie L (1998) Designer oils: the high oleic soybean. In: Roller S, Harlander S (eds) Genetic modification in the food industry. Blackie, London, pp 193–213
Kinney A, Cahoon E, Damude H (2004) Production of very long chain polyunsaturated fatty acids in oilseed plants. PCT Int ApplWO2004071467
Kitano H (2002) Systems biology: a brief overview. Science 295:1662–1664
Kok-Jacon G, Ji Q, Vincken J-P, Visser R (2003) Towards a more versatile α-glucan synthesis in plants. J Plant Physiol 160:765–777
Kondo T, Yoshida K, Nakagawa A, Kawai T, Tamura H, Goto T (1992) Structural basis of blue-color development in flower petals from Commelina communis. Nature 358:515–518
Kota M, Daniell H, Varma S, Garczynski SF, Gould F, Moar WJ (1999) Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects. Proc Natl Acad Sci USA 96:1840–1845
Kumar A, Altabella T, Taylor M (1997) Recent advances in polyamine research. Trends Plant Sci 2:124–130
Kumar S, Sharma M, Rajam M (2006) Polyamine biosynthetic pathway as a novel target for potential applications in plant biotechnology. Physiol Mol Biol Plant 12:53–58
Kumar G, Ganapathi T, Srinivas L, Bapat V (2007) Plant molecular farming: host systems, technology and products. In: Verpoorte R, Alfermann A, Johnson T (eds) Applications of plant metabolic engineering. Springer, Dordrecht, pp 45–77
Kurnaz I (2005) Biochemical modelling tools and applications to metabolic engineering. Turk J Biochem 30:200–207
Kusnadi A, Hood E, Witcher D (1998) Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog 14:149–155
Latchman D (2003) Eukaryotic transcription factors, 4th edn. Academic, San Diego, CA
Leelavathi S, Sunnichan VG, Kumria R, Vijaykanth GP, Bhatnagar RK, Reddy VS (2004) A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): embryogenic calli as a source to generate large numbers of transgenic plants. Plant Cell Rep 22:465–470
Li Z, Chen S (2000) Differential accumulation of the S-adenosylmethionine decarboxylase transcript in rice seedlings in response to salt and drought stress. Theor Appl Genet 100:782–788
Liu J-W, Huang Y-S, DeMichele S, Bergana M, Bobik EJ, Hastilow C, Chuang L-T, Mukerji P, Knutzon D (2001) Evaluation of the seed oils from a canola plant genetically transformed to produce high levels of γ-linoleic acid. In: Hunag Y-S, Ziboh V (eds) γ-linoleic acid: recent advances in biotechnology and clinical applications. AOCS Press, Champaign, IL, pp 61–71
Liu Q, Wang Z, Chen X, XL C, Tang S, Yu H, Zhang J, Hong M, Gu M (2003) Stable inheritance of the antisense Waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Res 12:71–82
Lossl A, Eibl C, Harloff HJ, Jung C, Koop HU (2003) Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction. Plant Cell Rep 21:891–899
Magnuson N, Linzmaier P, Reeves R (1998) Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Protein Expr Purif 13:45–52
Mallik V, Watson M, Malmberg R (1996) A tobacco ornithine decarboxylase partial cDNA clone. J Plant Biochem Biotechnol 5:109–112
McBride KE, Svab Z, Schaaf DJ, Hogan PS, Stalker DM, Maliga P (1995) Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticidal protein in tobacco. BioTechnology 13:362–365
Mehta R, Cassol T, Li N, Ali N, Handa A, Mattoo A (2002) Engineered polyamine accumulation in tomato enhances phytonutrient content, juice quality, and vine life. Nat Biotechnol 20:613–618
Meyer P, Heidmann I, Forkmann G, Saedler H (1987) A new petunia flower color generated by transformation of a mutant with a maize gene. Nature 330:677–678
Micheal A, Furze J, Rhodes M (1996) Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA. Biochem J 314:241–248
Mol J, Grotewold E, Koes R (1998) How genes paint flowers and seeds. Trends Plant Sci 3:212–217
Mol J, Cornish E, Mason J, Koes R (1999) Novel colored flowers. Curr Opin Biotechnol 10:198–201
Murai N, Kemp JD, Sutton DW, Murray MG, Slightom JL, Merlo DJ, Reichert NA, Sengupta-Gopalan C, Stock CA, Barker RF (1983) Phaseolin gene from bean is expressed after transfer to sunflower via tumor-inducing plasmid vectors. Science 222:476–482
Murphy D (2002) Biotechnology and the improvement of oil crops-genes, dreams and realities. Phytochem Rev 1:67–77
Nakashita H, Arai Y, Shikanai T, Doi Y, Yamaguchi I (2001) Introduction of bacterial metabolism into higher plants by polycistronic transgene expression. Biosci Biotechnol Biochem 65:1688–1691
Ososki AL, Kennelly EJ (2003) Phytoestrogens: a review of the present state of research. Phyt-other Res 17:845–869
Park W, Lee S, Park K (1998) Cloning and characterization of genome clone (Accession no. U64927) encoding S-adenosyl-L-methionine decarboxylase whose gene expression was regulated by light in morning glory (Ipomea nil L.). Plant Physiol 116:867–872
Pereira S, Leonard A, Huang Y-S (2004) Identification of two novel microalgal enzymes involved in the conversion of the ω3-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid. Biochem J 384:357–366
Perez-Amadour M, Carbonell J (1995) Arginine decarboxylase and putrescine oxidase in ovaries of Pissum sativum L. changes during ovary senescence and early fruit development. Plant Physiol 107:865–872
Persson S, Wei H, Milne J, Page GP, Somerville CR (2005) Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets. Proc Natl Acad Sci USA 102:8633–8638
Polashock JJ, Griesbach RJ, Sullivan RF, Vorsa N (2002) Cloning of a cDNA encoding the cranberry dihydroflavonol-4-reductase (DFR) and expression in transgenic tobacco. Plant Sci 163:241–251
Portis AR Jr, Parry MA (2007) Discoveries in Rubisco (Ribulose 1, 5-bisphosphate carboxylase/ oxygenase): a historical perspective. Photosynth Res 94:121–143
Pueppke SG (1996) The genetic and biochemical basis for nodulation of legumes by rhizobia. Crit Rev Biotechnol 16:1–51
Qi B, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier J, Stobart A, Lazarus C (2004) Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol 22:739–745
Quattrocchio F, Verweij W, Kroon A, Spelt C, Mol J, Koes R (2006) PH4 of Petunia is an R2R3 MYB protein that activates vacuolar acidification through interactions with basic-helix-loop-helix transcription factors of the anthocyanin pathway. Plant Cell 18:1274–1291
Quesada-Vargas T, Ruiz ON, Daniell H (2005) Characterization of heterologous multigene operons in transgenic chloroplasts: transcription, processing, and translation. Plant Physiol 138:1746–1762
Rajam M (1997) Polyamines. In: Prasad M (ed) Plant ecophysiology. Wiley, New York, pp 343–374
Rastogi R, Dulson J, Rothstein S (1993) Cloning of tomato (Lycopersicum esculentum Mill.) arginine decarboxylase gene and its expression during fruit ripening. Plant Physiol 103:829–834
Regierer B, Fernie A, Springer F, Perez-Melis A, Leisse A, Koehl K, Willmitzer L, Geigenberger P, Kossmann J (2002) Starch content and yield increase as a result of altering adenylate poolsin transgenic plants. Nat Biotechnol 20:1256–1260
Ritsema T, Smeekens S (2003) Fructans: beneficial for plants and humans. Curr Opin Plant Biol 6:223–230
Rivera-Vargas LI, Schmitthenner AF, Graham TL (1993) Soybean flavonoid effects on and metabolism by Phytophthora sojae. Phytochemistry 32:851–857
Rosche E, Blackmore D, Tegeder M,RichardsonT, Schroeder H, Higgins T, Frommer W, Offler C, Patrick J (2002) Seed-specific overexpression of the potato sucrose transporter increases sucrose uptake and growth rates of developing pea cotyledons. Plant J 30:165–175
Ruan Y, Llewellyn D, Furbank R (2003) Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell 15:952–964
Ruhlman T, Ahangari R, Devine A, Samsam M, Daniell H (2007) Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts—oral administration protects against development of insulitis in non-obese diabetic mice. Plant Biotechnol J 5:495–510
Sato S, Xing A, Ye X, Schweiger B, Kinney A, Graef G, Clemente T (2004) Production of γ-linoleic acid and stearidonic acid in seeds of marker-free transgenic soybean. Crop Sci 44:646–652
Schroeder G, Schroeder J (1995) cDNA for S-adenosyl-L-methionine decarboxylase from Catharanthus roseus, heterologous expression, identification of the proenzyme processing site, evidence for the presence of both subunits in the active enzyme and a conserved region in the 5′ messenger RNA leader. Eur J Biochem 228:74–78
Segal G, Song R, Messing J (2003) A new opaque variant of maize by a single dominant RNA-interference -inducing transgene. Genetics 165:387–397
Shiono M, Matsugaki N, Takeda K (2005) Phytochemistry: structure of the blue cornflower pigment. Nature 436:791
Shoji K, Miki N, Nakajima N, Momonoi K, Kato C, Yoshida K (2007) Perianth bottom-specific blue color development in Tulip cv. Murasakizuisho requires ferric ions. Plant Cell Physiol 48:243–251
Sijmons P, Dekker B, Schrammeijer B (1990) Production of correctly processed human serum albumin in transgenic plants. Biotechnology 8:217–221
Singh S, Zhou X, Liu Q, Stymne S, Green A (2005) Metabolic engineering of new fatty acids in plants. Curr Opin Plant Biol 8:197–203
Smidansky E, Clancy M, Meyer F, Lanning S, Blake N, Talbert L, Giroux M (2002) Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield. Proc Natl Acad Sci USA 99:1724–1729
Smidansky E, Martin J, Hannah L, Fischer A, Giroux M (2003) Seed yieldand plant biomass increases in rice are conferred by deregulation of endosperm ADP-glucose pyrophosphorylase. Planta 216:656–664
Spaink HP (2000) Root nodulation and infection factors produced by rhizobial bacteria. Annu Rev Microbiol 54:257–288
Stafford HA (1990) Flavonoid metabolism. CRC Press, Boca Raton FL
Staub JM, Garcia B, Graves J, Hajdukiewicz PT, Hunter P, Nehra N, Paradkar V, Schlittler M, Carroll JA, Spatola L, Ward D, Ye G, Russell DA (2000) High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat Biotechnol 18:333–338
Steele CL, Gijzen M, Qutob D, Dixon RA (1999) Molecular characterization of the enzyme catalyzing the aryl migration reaction of isoflavonoid biosynthesis in Soybean. Arch Biochem Biophys 367:146–150
Stephanopoulos G, Vallino JJ (1991) Network rigidity and metabolic engineering in metabolite overproduction. Science 252:1675–1681
Sticklen M (2008) Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat Rev Genet 9:433–443
Sticklen M, Dale B, Maqbool S (2006) Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars. US Patent 7,049,485
Svab Z, Hajdukiewicz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87:8526–8530
Sweetlove LJ, Last RL, Fernie AR (2003) Predictive metabolic engineering: a goal for systems biology. Plant Physiol 132:420–425
Tanaka Y (2006) Flower color and cytochromes P450. Phytochem Rev 5:283–291
Tanaka Y, Brugliera F (2006) Flower color. In: Ainsworth C (ed) Flowering and its manipulation. Blackwell, Oxford, pp 201–239
Tanaka Y, Tsuda S, Kusumi T (1998) Metabolic engineering to modify flower color. Plant Cell Physiol 39:1119–1126
Taylor M, Mad Arif S, Kumar A (1992) Expression and sequence analysis of cDNAs induced during the early stages of tuberization in different organs of potato plant (Solanum tuberosum L.). Plant Mol Biol 20:641–651
Thelen J, Ohlrogge J (2002) Metabolic engineering of fatty acid biosynthesis in plants. Metabol Eng 4:12–21
Thu-Hang P, Bassie L, Safwat G, Trung-Nghia P, Christou P, Capell T (2002) Expression of a heterologous S-adenosylmethionine decarboxylase cDNA in plants demonstrates that changes in S-adenosyl-L-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine. Plant Physiol 129:1744–1754
Tian L, Dixon RA (2006) Engineering isoflavone metabolism with an artificial bifunctional enzyme. Planta 224:496–507
Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F, Fairweather N, Ytterberg J, van Wijk KJ, Dougan G, Maliga P (2003) Expression of tetanus toxin Fragment C in tobacco chloroplasts. Nucl Acids Res 31:1174–1179
Trung-Nghia P, Bassie L, Safwat G, Thu-Hang P, Lepri O, Rocha P, Christou P, Capell T (2003) Reduction in the endogenous arginine decarboxylase transcript levels in rice leads to depletion of the putrescine and spermidine pools with no concomitant changes in the expression of downstream genes in the polyamine biosynthetic pathway. Planta 218:125–134
Ueyama Y, Suzuki K, Fukuchi-Mizutani M, Fukui Y, Miyazaki K, Ohkawa H, Kusumi T, Tanaka Y (2002) Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genes. Plant Sci 163:253–263
Van Eenennaam A, Lincoln K, Durrett T, Valentin H, Shewmaker C, Thome G, Jiang J, Baszis S, Levering C, Aasen E (2003) Engineering vitamin E content: from Arabidopsis mutant to soy oil. Plant cell 15:3007–3019
Verweij W, Spelt C, Di Sansebastiano G-P, Vermeer J, Reale L, Ferranti F, Koes R, Quattrocchio F (2008) An H+ P-ATPase on the tonoplast determines vacuolar pH and flower color. Nat Cell Biol 10:1456–1462
Villarejo A, Buren S, Larsson S, Dejardin A, Monne M, Rudhe C, Karlsson J, Jansson S, Lerouge P, Rolland N, von Heijne G, Grebe M, Bako L, Samuelsson G (2005) Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast. Nat Cell Biol 7:1224–1231
Vincken Q, Suurs L, Visser R (2003) Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Mol Biol 51:789–801
Watson M, Malmberg R (1996) Regulation of Arabidopsis thaliana (L.) arginine decarboxylase by potassium deficiency stress. Plant Physiol 111:1077–1083
Whitney SM, Andrews TJ (2003) Photosynthesis and growth of tobacco with a substituted bacterial Rubisco mirror the properties of the introduced enzyme. Plant Physiol 133:287–294
Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126:485–493
Witcher D, Hood E, Petersen D (1998) Commercial production of b-glucuronidase (GUS): a model system for the production of proteins in plants. Mol Breed 4:301–312
Wurbs D, Ruf S, Bock R (2007) Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome. Plant J 49:276–288
Yabuya T, Nakamura M, Iwashina T, Yamaguchi M, Takehara T (1997) Anthocyanin-flavone copigmentation in bluish purple flowers of Japanese garden iris (Iris ensata Thunb.). Euphytica 98:163–167
Yadav N, Wierzbicki A, Aegerter M (1993) Cloning of higher plant ω-3 fatty acid desaturases. Plant Physiol 103:467–476
Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305
Ye GN, Hajdukiewicz PT, Broyles D, Rodriguez D, Xu CW, Nehra N, Staub JM (2001) Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. Plant J 25:261–270
Yoshida K, Kondo T, Okazaki Y, Katou K (1995) Cause of blue petal color. Nature 373:291
Yoshida K, Toyama-Kato Y, Kameda K, Kondo T (2003) Sepal color variation of Hydrangea macrophylla and vacuolar pH measured with a proton-selective microelectrode. Plant Cell Physiol 44:262–268
Yu O, Jung W, Shi J, Croes RA, Fader GM, McGonigle B, Odell JT (2000) Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues. Plant Physiol 124:781–793
Yu O, Shi J, Hession AO, Maxwell CA, McGonigle B, Odell JT (2003) Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63:753–763
Zhong R, Morrison W, Freshour G, Hahn M, Ye Z (2003) Expression of a mutant formof cellulose synthase AtCesA7 causes dominant negative effect on cellulose biosynthesis. Plant Physiol 129:797–807
Zhu X, Galili G (2003) Increased lysine synthesis coupled with a knockout of its catabolism synergistically boosts lysine content and also transregulates the metabolism of other amino acids in Arabidopsis seeds. Plant cell 15:845–853
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Juříček, M., Emani, C., Kertbundit, S., Hall, T.C. (2010). Metabolic Engineering of Pathways and Gene Discovery. In: Kole, C., Michler, C.H., Abbott, A.G., Hall, T.C. (eds) Transgenic Crop Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04809-8_10
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