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Cotton Transformation

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Cotton

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 65))

Abstract

Cotton has been cultured in vitro for 37 years, yet even today, some of the culture difficulties that were seen when it was first cultured, namely, a very long culture time and a limited number of cultivars that can be cultured, continue to pose problems. These inherent tissue culture limitations have made transforming cotton an arduous process. Transforming embryogenic callus was a major step towards reducing the time required to produce a transgenic event. Initial success transforming seed-derived apical meristems suggests that the major drawbacks to cotton tissue culture and transformation may be overcome, but the process potentially creates chimeric plants. Pollen or floral-type transformations of cotton eliminate meristem transformation-derived chimerism but require substantial space for plants, time, and labor. For both meristem and pollen or floral-type transformations to become practical options for the routine transformation of cotton, the efficiency and ease of performing these processes must be increased dramatically.

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  1. Monsanto Company, 700 Chesterfield Parkway W, Chesterfield, MO, USA

    D. R. Duncan

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Duncan, D.R. (2010). Cotton Transformation. In: Cotton. Biotechnology in Agriculture and Forestry, vol 65. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04796-1_4

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