Abstract
Immunohistochemistry at the ultrastructural level, also known as immunoelectron microscopy, can be performed on ultrathin cryosections or on ultrathin sections of probes embedded in epoxy or acrylic resins. Ultrathin frozen sections are prepared from aldehyde-fixed specimens that have been infused with cryoprotectants such as sucrose and then frozen in liquid nitrogen. After freezing, the sections are cut using glass or diamond knives at between −90°C and −120°C. They are then thawed, mounted on plastic-coated EM grids and immunolabeled. The review of the theoretical background, equipment, methods, and applications of cryofixation and cryoultramicrotomy (Saga 2005) can be found in the Internet under address: http://www.springerlink.com/content/x763346q14552851/fulltext.pdf.
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Buchwalow, I.B., Böcker, W. (2010). Immunohistochemistry at the Ultrastructural Level. In: Immunohistochemistry: Basics and Methods. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-04609-4_12
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DOI: https://doi.org/10.1007/978-3-642-04609-4_12
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Online ISBN: 978-3-642-04609-4
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