Abstract
The radioprotective effect of hesperidin against genotoxicity induced by gamma irradiation has been investigated in vivo/ in vitro in cultured blood lymphocytes from human volunteers. Peripheral blood samples were collected from human volunteers at 0 (10min before), and at 1, 2 and 3 h after a single oral ingestion of 250 mg hesperidin. At each time point, the whole blood was exposed in vitro to 150 cGy of cobalt-60 gamma irradiation, and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis blocked binucleated cell.
For each volunteer, the results showed a significant increase in the incidence of micronuclei after exposed to gamma irradiation as compared to control samples. The lymphocytes in the blood samples collected at 1 h after hesperidin ingestion and exposed in vitro to gamma rays exhibited a significant decrease in the incidence of micronuclei, as compared with similarly irradiated lymphocytes from the blood sample collected at 0 h. The maximum protection and decrease in frequency of micronuclei was observed at 1 h after ingestion of hesperidin with 33%.
This data have important application for the protection of human lymphocyte from the genetic damage and side effects induced by gamma irradiation in patients undergoing radiotherapy.
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© 2009 Springer-Verlag Berlin Heidelberg
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Hosseinimehr, S.J., Mahmoudzadeh, A., Ahmadi, A., Mohamadifar, S., Akhlaghpoor, S. (2009). Radioprotective Effects of Hesperidin against Genotoxicity Induced by Gamma Irradiation in Human Lymphocytes. In: Dössel, O., Schlegel, W.C. (eds) World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany. IFMBE Proceedings, vol 25/3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03902-7_5
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DOI: https://doi.org/10.1007/978-3-642-03902-7_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-03901-0
Online ISBN: 978-3-642-03902-7
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