Abstract
The aim of this pilot study was to elucidate the potential of 40-MHz ultrasound to monitor the evolution of matrix synthesis by human chondrocytes seeded in collagen I/III scaffolds. Volumetric ultrasound data were acquired in empty scaffolds and at time points of 14, 28 and 42 days of culture. Surface reflection, acoustic backscatter and bulk properties (sound velocity and attenuation) of the tissue constructs were assessed by spectral analysis of the radio frequency data and compared to quantitative proteoglycan deposition and standard histological analyses. Several spectral backscatter parameters were linked to an increasing deposition of neocartilage. These preliminary data suggest that high frequency ultrasound biomicroscopy is a suitable method to monitor matrix synthesis in tissue-engineered scaffolds.
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© 2009 Springer-Verlag Berlin Heidelberg
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Männicke, N., Raum, K., Richter, W., Steck, E. (2009). Monitoring of cartilage synthesis in tissue-engineered scaffolds by ultrasound biomicroscopy. In: Dössel, O., Schlegel, W.C. (eds) World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany. IFMBE Proceedings, vol 25/10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03900-3_50
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DOI: https://doi.org/10.1007/978-3-642-03900-3_50
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-03899-0
Online ISBN: 978-3-642-03900-3
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