Abstract
The opening step for any prevention strategy for skin sensitization is the identification of new allergens, ideally before they are put into the market, though already present in consumer products. Identifying contact allergens among the myriad of chemicals used in commercial products can be an extremely arduous task. In the case of fragrance allergy, for instance, approximately 3,000 ingredients, of synthetic origin or natural products, are available to the perfumer for compounding a fragrance formula, which may consist of 10–300 different ingredients in a cosmetic product. In this chapter, a methodology based on the combination of bioassay-guided chemical fractionation, patch test and/or use testing of individuals with fractions, detailed chemical composition analysis of positive fractions, and chemical structure-activity relationship analysis of identified molecules is presented as a valuable tool for the identification of allergens in complex mixtures. Practical applications such as the cases of eaux de toilette and the allergenic natural extract oak moss are detailed.
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Giménez-Arnau, E. (2011). Bio-Guided Fractionation and Identification of Allergens in Complex Mixtures and Products. In: Johansen, J., Frosch, P., Lepoittevin, JP. (eds) Contact Dermatitis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03827-3_5
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DOI: https://doi.org/10.1007/978-3-642-03827-3_5
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