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Contact Dermatitis pp 111–119Cite as

Bio-Guided Fractionation and Identification of Allergens in Complex Mixtures and Products

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Abstract

The opening step for any prevention strategy for skin sensitization is the identification of new allergens, ideally before they are put into the market, though already present in consumer products. Identifying contact allergens among the myriad of chemicals used in commercial products can be an extremely arduous task. In the case of fragrance allergy, for instance, approximately 3,000 ingredients, of synthetic origin or natural products, are available to the perfumer for compounding a fragrance formula, which may consist of 10–300 different ingredients in a cosmetic product. In this chapter, a methodology based on the combination of bioassay-guided chemical fractionation, patch test and/or use testing of individuals with fractions, detailed chemical composition analysis of positive fractions, and chemical structure-activity relationship analysis of identified molecules is presented as a valuable tool for the identification of allergens in complex mixtures. Practical applications such as the cases of eaux de toilette and the allergenic natural extract oak moss are detailed.

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Authors and Affiliations

  1. Laboratoire de Dermatochimie, Institut de Chimie de Strasbourg – ILB, 4, Rue Blaise Pascal, 67070, Strasbourg, France

    Elena Giménez-Arnau

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  1. Elena Giménez-Arnau
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Correspondence to Elena Giménez-Arnau .

Editor information

Editors and Affiliations

  1. Dept. Dermatology, Amtssygehuset Gentofte, Niels Andersens Vej 65, Hellerup, 2900, Denmark

    Jeanne Duus Johansen

  2. Klinikzentrum Mitte, Hautklinik, Städtische Kliniken Dortmund, Beurhausstr. 40, Dortmund, 44137, Germany

    Peter J. Frosch

  3. Labo. Dermatochimie, Institut le Bel, rue Blaise Pascal 4, Strasbourg CX, 67070, France

    Jean-Pierre Lepoittevin

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Giménez-Arnau, E. (2011). Bio-Guided Fractionation and Identification of Allergens in Complex Mixtures and Products. In: Johansen, J., Frosch, P., Lepoittevin, JP. (eds) Contact Dermatitis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03827-3_5

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  • DOI: https://doi.org/10.1007/978-3-642-03827-3_5

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-03826-6

  • Online ISBN: 978-3-642-03827-3

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