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Plastics from Bacteria pp 63–84Cite as

Towards Systems Metabolic Engineering of PHA Producers

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Part of the book series: Microbiology Monographs ((MICROMONO,volume 14))

Abstract

Polyhydroxyalkanoates (PHAs) are natural polyesters that accumulate in numerous microorganisms as a carbon- and energy-storage material under the nutrient-limiting condition in the presence of an excess carbon source. PHAs are considered to be one of the potential alternatives to petrochemically derived plastics owing to their versatile material properties. Over the past few decades, extensive detailed biochemical, molecular-biological, and metabolic studies related to PHA biosynthesis have been carried out. Advances in our knowledge of PHA biosynthesis led to the development of engineered strains and fermentation processes for the production of PHAs with high efficiency. Even though the traditional metabolic engineering based on our rational thinking and trial-and-error type approaches allowed development of improved strains, further enhancement in the performance is expected through systems metabolic engineering, which is metabolic engineering integrated with systems-biological approaches. In this chapter, the strategies taken for the metabolic engineering of PHA producers are briefly reviewed. Then, genomic and proteomic studies performed to understand the PHA biosynthesis in the context of whole cell metabolism as well as to develop further engineered strains are reviewed. Finally, the strategies for the systems metabolic engineering of PHA producers are suggested; these will make it possible to produce PHAs with higher efficiencies and to develop tailor-made PHAs by systems-level optimization of the metabolic network and establishment of novel pathways.

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Acknowledgements

Our work described in this paper was supported by the Korean Systems Biology Research Project (M10309020000–03B5002–00000) of the Ministry of Education, Science and Technology. Further support by the LG Chem Chair Professorship and Microsoft is appreciated.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea

    Yu Kyung Jung, Sang Yup Lee & Tran Thanh Tam

  2. Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea

    Yu Kyung Jung, Sang Yup Lee & Tran Thanh Tam

  3. Department of Bio and Brain Engineering, BioProcess Engineering Research Center and Bioinformatics Research Center, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

  4. Department of Biological Sciences, KAIST, 335 Gwahangno, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

Authors
  1. Yu Kyung Jung
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  2. Sang Yup Lee
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  3. Tran Thanh Tam
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Corresponding author

Correspondence to Sang Yup Lee .

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Editors and Affiliations

  1. Dept. Biological Sciences, Tsinghua University, Beijing, 100084, China, People's Republic

    George Guo-Qiang Chen

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Jung, Y.K., Lee, S.Y., Tam, T.T. (2010). Towards Systems Metabolic Engineering of PHA Producers. In: Chen, GQ. (eds) Plastics from Bacteria. Microbiology Monographs, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-03287-5_4

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