Abstract
Pluripotent human stem cells, including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, hold tremendous promise as a source of progenitor cells and terminally differentiated cells in tissue engineering and regenerative medicine applications. Pluripotent stem cells are capable of unlimited self-renewal and have the ability to differentiate to clinically relevant cell types in each of the three germ lineages. ES cells were first derived from the inner cell mass of the blastocyst, while iPS cells can be generated from differentiated cells by stimulating expression of transcription factors that regulate pluripotency. This chapter discusses advances in deriving, characterizing, and culturing pluripotent human stem cell lines, with a focus on using these lines as a cell source for tissue engineering applications.
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Acknowledgments
The author thanks members of his lab, especially Samira Azarin, for helpful discussions. Support for this chapter was provided by the National Science Foundation grant EFRI-0735903 and National Institute of Biomedical Imaging and Bioengineering grant R01EB007534.
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Palecek, S.P. (2011). Pluripotent Stem Cells: Sources and Characterization. In: Pallua, N., Suscheck, C. (eds) Tissue Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02824-3_4
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