Abstract
A series of advances over the past decade have made the combination of coherent Raman scattering (CRS) with optical microscopy a highly sensitive and chemically selective tool for the label-free and noninvasive analysis of chemical species or biological components inside a sub-femtoliter probe volume. By exploiting the coherent driving and detection of Raman modes in coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), CRS imaging allows the point-by-point chemical mapping of living cells and tissues in three dimensions and in real time, which is often difficult to attain by fluorescence and incoherent vibrational microscopy techniques. Beyond imaging, CRS microspectroscopy in the frequencyand time-domain provides fast access to the full wealth of the sample’s spontaneous Raman response. In this review, we give an overview of the common fundamental aspects of CARS and SRS microscopy, their distinct image contrast mechanisms and microspectroscopy implementations, together with emerging biomedical applications.
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Acknowledgments
I would like to extent a special thanks to my former colleagues X. Sunney Xie, Gary R. Holtom, Ji-Xin Cheng, and Lewis D. Book at Harvard University where the work on CARS microscopy described in this review had its origin. In equal measure, I wish to acknowledge my former and past group members Nandakumar Patincharath, Alexander Kovalev, Adam Muschielok, Stephan Busch, and Gregor Hehl at the University of Stuttgart for their essential contributions to the depicted work on in vivo CARS microspectroscopy and SRS imaging. Financial and infrastructural support from the Emmy-Noether program of the Deutsche Forschungsgemeinschaft (DFG: VO 825/1-2, 1-3, 1-4), the 3. Physikalisches Institut, and the University of Stuttgart is gratefully acknowledged.
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Volkmer, A. (2010). Coherent Raman Scattering Microscopy. In: Matousek, P., Morris, M. (eds) Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02649-2_6
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