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Single Molecule Spectroscopy in Chemistry, Physics and Biology pp 305–316Cite as

Single Cell Physiology

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Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 96))

Summary

The possibility to control at specific times and specific places the activity of biomolecules (enzymes, transcription factors, RNA, hormones, etc.) is opening up new opportunities in the study of physiological processes at the single cell level in a live organism. Most existing gene expression systems allow for tissue specific induction upon feeding the organism with exogenous inducers (e.g., tetracycline). Local genetic control has earlier been achieved by micro-injection of the relevant inducer/repressor molecule, but this is an invasive and possibly traumatic technique. In this chapter, we present the requirements for a noninvasive optical control of the activity of biomolecules and review the recent advances in this new field of research.

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Acknowledgements

We thank Frédéric Rosa and Shuo Lin for access to their fish facilities, and Laure Bally-Cuif and Christof Leucht for the gift of the zebrafish line used for the Cre reporter experiments. This work has been supported by Association pour la Recherche sur le Cancer. PN research is supported in part by the National Science Foundation under Grant No. PHY05-51164. DKS acknowledges support from the NABI CNRS-Weizmann Institute program and DB the partial support of a PUF ENS-UCLA grant.

Author information

Authors and Affiliations

  1. Kavli Institute for Theoretical Physics, University of California at Santa Barbara, Santa Barbara, CA, USA

    Pierre Neveu

  2. Laboratoire de Physique Statistique UMR 8550 Ecole Normale Supérieure, Paris, France

    Deepak Kumar Sinha

  3. Department of Physiological Science, University of California at Los Angeles, Los Angeles, CA, USA

    Petronella Kettunen

  4. Inserm U770 Hémostase et Dynamique Cellulaire Vasculaire, Le Kremlin-Bicêtre, France

    Sophie Vriz

  5. Département de Chimie UMR 8640 Ecole Normale Supérieure, Paris, France

    Ludovic Jullien & David Bensimon

  6. Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, USA

    David Bensimon

Authors
  1. Pierre Neveu
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  2. Deepak Kumar Sinha
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  3. Petronella Kettunen
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  4. Sophie Vriz
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  5. Ludovic Jullien
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  6. David Bensimon
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Corresponding author

Correspondence to Pierre Neveu .

Editor information

Editors and Affiliations

  1. Dept. Biophysics, Stockholm University, Stockholm, 106 91, Sweden

    Astrid Gräslund

  2. rue du Centre 115, St. Sulpice, 1025, Switzerland

    Rudolf Rigler

  3. Dept. Biomolecular Physics, Royal Institute of Technology (KTH), Stockholm, 106 91, Sweden

    Jerker Widengren

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Neveu, P., Sinha, D.K., Kettunen, P., Vriz, S., Jullien, L., Bensimon, D. (2010). Single Cell Physiology. In: Gräslund, A., Rigler, R., Widengren, J. (eds) Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02597-6_16

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  • DOI: https://doi.org/10.1007/978-3-642-02597-6_16

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-02596-9

  • Online ISBN: 978-3-642-02597-6

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