Summary
The possibility to control at specific times and specific places the activity of biomolecules (enzymes, transcription factors, RNA, hormones, etc.) is opening up new opportunities in the study of physiological processes at the single cell level in a live organism. Most existing gene expression systems allow for tissue specific induction upon feeding the organism with exogenous inducers (e.g., tetracycline). Local genetic control has earlier been achieved by micro-injection of the relevant inducer/repressor molecule, but this is an invasive and possibly traumatic technique. In this chapter, we present the requirements for a noninvasive optical control of the activity of biomolecules and review the recent advances in this new field of research.
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Acknowledgements
We thank Frédéric Rosa and Shuo Lin for access to their fish facilities, and Laure Bally-Cuif and Christof Leucht for the gift of the zebrafish line used for the Cre reporter experiments. This work has been supported by Association pour la Recherche sur le Cancer. PN research is supported in part by the National Science Foundation under Grant No. PHY05-51164. DKS acknowledges support from the NABI CNRS-Weizmann Institute program and DB the partial support of a PUF ENS-UCLA grant.
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Neveu, P., Sinha, D.K., Kettunen, P., Vriz, S., Jullien, L., Bensimon, D. (2010). Single Cell Physiology. In: Gräslund, A., Rigler, R., Widengren, J. (eds) Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02597-6_16
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