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Size-Minimized Quantum Dots for Molecular and Cellular Imaging

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Single Molecule Spectroscopy in Chemistry, Physics and Biology

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 96))

Summary

Semiconductor quantum dots, tiny light-emitting particles on thenanometer scale, are emerging as a new class of fluorescent labels for a broad range of molecular and cellular applications. In comparison with organic dyes and fluorescent proteins, they have unique optical and electronic properties such as size-tunable light emission, intense signal brightness, resistance to photobleaching, and broadband absorption for simultaneous excitation of multiple fluorescence colors. Here we report new advances in minimizing the hydrodynamic sizes of quantum dots using multidentate and multifunctional polymer coatings. A key finding is that a linear polymer containing grafted amine and thiol coordinating groups can coat nanocrystals and lead to a highly compact size, exceptional colloidal stability, strong resistance to photobleaching, and high fluorescence quantum yields. This has allowed a new generation of bright and stable quantum dots with small hydrodynamic diameters between 5.6 and 9.7 nm with tunable fluorescence emission from the visible (515 nm) to the near infrared (720 nm). These quantum dots are well suited for molecular and cellular imaging applications in which the nanoparticle hydrodynamic size needs to be minimized. Together with the novel properties of new strain-tunable quantum dots, these findings will be especially useful for multicolor and super-resolution imaging at the single-molecule level.

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Acknowledgements

This work was supported by grants from the National Institutes of Health (P20 GM072069, R01 CA108468, and U01HL080711, U54CA119338). A.M.S. acknowledges the Whitaker Foundation for generous fellowship support.

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Authors and Affiliations

  1. Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 101 Woodruff Circle Suite 2001, Atlanta, GA, 30322, USA

    Andrew M. Smith, Mary M. Wen & Shuming Nie

  2. Departments of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive UA Whitaker Building 4106, Atlanta, GA, 30322, USA

    May D. Wang

Authors
  1. Andrew M. Smith
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  2. Mary M. Wen
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  4. Shuming Nie
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Corresponding author

Correspondence to Shuming Nie .

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Editors and Affiliations

  1. Dept. Biophysics, Stockholm University, Stockholm, 106 91, Sweden

    Astrid Gräslund

  2. rue du Centre 115, St. Sulpice, 1025, Switzerland

    Rudolf Rigler

  3. Dept. Biomolecular Physics, Royal Institute of Technology (KTH), Stockholm, 106 91, Sweden

    Jerker Widengren

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Smith, A.M., Wen, M.M., Wang, M.D., Nie, S. (2010). Size-Minimized Quantum Dots for Molecular and Cellular Imaging. In: Gräslund, A., Rigler, R., Widengren, J. (eds) Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02597-6_10

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  • DOI: https://doi.org/10.1007/978-3-642-02597-6_10

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