Herbicide Resistance

  • Micheal D. K. OwenEmail author
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 64)


This is a general review of the global adoption of genetically modified herbicide-resistant crops and the implications that these transgenic crops have on weed populations. Topics discussed include: aspects of the evolution of herbicide-resistant weed biotypes, the mechanisms of resistance and interaction with transgenes. Finally, a brief discussion is developed about the management tactics used in genetically modified herbicide resistant crops and the impact these tactics have on herbicide use, integrated weed management and the environment. While the adoption of genetically modified herbicide crops represents an unprecedented and globally important revolution in agriculture, both the benefits and the risks of these transgenic crops must be considered objectively. Generally, a position is offered that the benefits of the genetically modified herbicide resistant crops outweigh the risks of their cultivation. However, depending on the crop species, location of production and management system employed, the risks attributable to crop production systems based on genetically modified herbicide resistant crops must not be ignored and appropriate adjustments of the management tactics must be implemented in order to mitigate the risks.


Weed Species Herbicide Resistance Weed Management Weed Population Management Tactic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Abud S, de Souza PIM, et al (2007) Gene flow from transgenic to nontransgenic soybean plants in the Cerrado region of Brazil. Gen Mol Res 6:445–452Google Scholar
  2. Ammann K (2005) Effects of biotechnology on biodiversity: herbicide-tolerant and insect-resistant GM crops. Trends Biotechnol 23:388–394PubMedCrossRefGoogle Scholar
  3. Anonymous (2002) Oregon administrative rules, 2002. Oregon State Archives 603-052-1240. Accessed 15 Oct 2006
  4. Anonymous (2004) National crop residue management survey data. Accessed 15 June 2007
  5. Anonymous (2006) National agricultural statistics service acreage report. United States Department of Agriculture, Washington, D.C.Google Scholar
  6. Baerson SR, Rodiguez DJ, et al (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:1265–1275PubMedCrossRefGoogle Scholar
  7. Bauer TA, Mortensen DA (1992) A comparison of economic and economic optimum thresholds for two annual weeds in soybeans. Weed Technol 6:228–235Google Scholar
  8. Beckie HJ, Owen MDK (2007) Herbicide-resistant crops as weeds in north America. (CAB reviews 2: perspectives in agriculture, veterinary science, nutrition, and natural resources) CAB Rev 2:22Google Scholar
  9. Behrens MR, Mutlu N, et al (2007) Dicamba resistance: enlarging and preserving biotechnology-based weed management strategies. Science 316:1185–1188Google Scholar
  10. Benbrook CM (2001) Do GM crops mean less pesticide use? Pest Outlook 12:204–207CrossRefGoogle Scholar
  11. Boerboom C (2008) Glyphosate resistant weed update. Wisconsin fertilizer, aglime and pest management conference. University of Wisconsin, Madison, pp 102–110Google Scholar
  12. Boerboom C, Sprague C, et al (2009) A grower's conundrum: Implementing integrated weed management in a HRC world. Int IPM Symp 6:24Google Scholar
  13. Bonny S (2007) Genetically modified glyphosate-tolerant soybean in the USA: adoption factors, impacts and prospects. A review. Agron Sustain Dev 28:21–32CrossRefGoogle Scholar
  14. Bradshaw LD, Padgette SR, et al (1997) Perspectives on glyphosate resistance. Weed Technol 11:189–198Google Scholar
  15. CaJacob CA, Feng PCC, et al (2007). Genetically modified herbicide-resistant crops. In: Kramer W, Schirmer U (eds) Modern crop protection chemicals, vol 1. Wiley-VCH, Weinheim, pp 283–302Google Scholar
  16. Carpenter J, Gianessi L (1999) Herbicide tolerant soybeans: why growers are adopting Roundup Ready varieties. AgBioForum 2:65–72Google Scholar
  17. Castle LA, Siehl DL, et al (2004) Discovery and directed evolution of a glyphosate tolerance gene. Science 304:1151–1154PubMedCrossRefGoogle Scholar
  18. Cattaneo MG, Yafuso C, et al (2006) Farm-scale evaluation of the impacts of transgenic cotton on biodiversity, pesticide use, and yield. Proc Natl Acad Sci USA 103:7571–7576PubMedCrossRefGoogle Scholar
  19. Cerdeira AL, Duke SO (2006) The current status and environmental impacts of glyphosate-resistant crops; a review. J Environ Qual 35:1633–1658PubMedCrossRefGoogle Scholar
  20. Charles D (2007) US courts say transgenic crops need tighter scrutiny. Science 315:1069PubMedCrossRefGoogle Scholar
  21. Dill GM (2005) Glyphosate-resistant crops; history, status and future. Pest Manage Sci 61:219–224CrossRefGoogle Scholar
  22. Dill GM, CaJacob CA, et al (2008) Glyphosate-resistant crops: adoption, use and future considerations. Pest Manage Sci 64:326–331CrossRefGoogle Scholar
  23. Duke SO (2005) Taking stock of herbicide-resistant crops ten years after introduction. Pest Manage Sci 61:211–218CrossRefGoogle Scholar
  24. Duke SO, Powles SB (2008) Glyphosate: a once-in-a-century herbicide. Pest Manage Sci 64:319–325CrossRefGoogle Scholar
  25. Fawcett R, Towery D (2004) Conservation tillage and plant biotechnology: how new technologies can improve the environment by reducing the need to plow. Conservation Technology Information Center, West Lafayette, p. 20Google Scholar
  26. Feng PCC, Tran M, et al (2004) Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498–505CrossRefGoogle Scholar
  27. Fisher L (2007) Growers continue to grow and use Roundup Ready alfalfa but Monsanto company is disappointed with preliminary injunction affecting purchase and planting. Accessed 8 March 2007
  28. Gealy DR, Dilday RH (1997) Biology of red rice (Oyrza sativa L.) accessions and their susceptibility to glufosinate and other herbicides. Weed Science Society of America/Allen, LawrenceGoogle Scholar
  29. Gepts P, Papa R (2003) Possible effects of (trans)gene flow from crops on the genetic diversity from landraces and wild relatives. Environ Biosaf Res 2:89–103CrossRefGoogle Scholar
  30. Gianessi LP (2005) Economic and herbicide use impacts of glyphosate-resistant crops. Pest Manage Sci 61:241–245CrossRefGoogle Scholar
  31. Gianessi LP, Reigner NP (2007) The value of herbicides in US crop production. Weed Technol 21:559–566CrossRefGoogle Scholar
  32. Green JM (2007) Review of glyphosate and ALS-inhibiting herbicide crop resistance and resistant weed management. Weed Technol 21:547–558CrossRefGoogle Scholar
  33. Green JM (2009) Evolution of glyphosate-resistant crop technology. Weed Sci 57:108–117CrossRefGoogle Scholar
  34. Green JM, Hazel CB, et al (2008) New multiple-herbicide crop resistance and formulation technology to augment the utility of glyphosate. Pest Manage Sci 64:332–339CrossRefGoogle Scholar
  35. Green JM, Hale T, et al (2009) Response of 98140 corn with gat4621 and hra transgenes to glyphosate and ALS-inhibiting herbicides. Weed Sci 57:142–148CrossRefGoogle Scholar
  36. Gressel J (1995) Creeping resistances: the outcome of using marginally effective or reduced rates of herbicides. Br Crop Protect Conf Weeds 1995: 587–589Google Scholar
  37. Gressel J (1996) Fewer constraints than proclaimed to the evolution of glyphosate-resistant weeds. Resist Pest Manage 8:2–5Google Scholar
  38. Gressel J, Levy AA (2006) Agriculture: the selector of improbable mutations. Proc Natl Acad Sci USA 103:12215–12216PubMedCrossRefGoogle Scholar
  39. Harper JL (1956) The evolution of weeds in relation to resistance to herbicides. Br Weed Control Conf 3:179–188Google Scholar
  40. Harriman P (2007) Roundup Ready concerns land alfalfa seed in court. Accessed 8 Mar 2007
  41. Hauser TP, Damgaard C, et al (2003) Frequency-dependent fitness of hybrids between oilseed rape (Brassica napus) and weedy B. rapa (Brassicacaeae). Am J Bot 90:571–578PubMedCrossRefGoogle Scholar
  42. Heap I (2009) The international survey of herbicide resistant weeds. Accessed 21 Jun 2009
  43. Hinz JJR, Owen MDK (1997) Acetolactate synthase resistance in a common waterhemp (Amaranthus rudis) population. Weed Technol 11:13–18Google Scholar
  44. James C (2008) Global status of commercialized biotech/GM crops: 2007. Int Serv Acquis AgriBiotech Appl 2008:12Google Scholar
  45. Johnson WG, Owen MDK, et al (2009) Farmer attitudes toward impending problems with genetically engineered glyphosate resistant crops may endanger the sustainability of chemically based weed management. Weed Technol 23:308–312CrossRefGoogle Scholar
  46. Kruger GR, Johnson WG, et al (2009) US grower views on problematic weeds and changes in weed pressure in glyphosate-resistant corn, cotton, and soybean cropping systems. Weed Technol 23:162–166CrossRefGoogle Scholar
  47. Legere A (2005) Risks and consequences of gene flow from herbicide-resistant crops: canola (Brassica napus L.) as a case study. Pest Manage Sci 61:292–300CrossRefGoogle Scholar
  48. Legleiter TR, Bradley KW (2008) Glyphosate and multiple herbicide resistance in common waterhemp (Amaranthus rudis) populations from Missouri. Weed Sci 56:582–587CrossRefGoogle Scholar
  49. Lu B-R (2004) Conserving biodiversity of soybean gene pool in the biotechnology era. Plant Species Biol 19:115–125CrossRefGoogle Scholar
  50. Lu Y-P, Li Z-S, et al (1997) AtMRP1 gene of Arabidopsis encodes a glutathione-S-conjugate pump: Isolation and functional definition of a plant ATP-binding cassette transporter gene. Proc Natl Acad Sci USA 94:8243–8248PubMedCrossRefGoogle Scholar
  51. Lydon J, Duke SO (1999). Inhibitors of glutamine biosynthesis. In: Singh BK (ed) Plant amino acids: biochemistry and biotechnology. Dekker, New York, pp 445–464Google Scholar
  52. Mallory-Smith C, Zapiola M (2008) Gene flow from glyphosate-resistant crops. Pest Manage Sci 64:428–440CrossRefGoogle Scholar
  53. Maxwell B, Jasieniuk M (2000) The evolution of herbicide resistance evolution models. Int Weed Sci Congr 3:172Google Scholar
  54. Mueller TC, Mitchell PD, et al (2005) Proactive versus reactive management of glyphosate-resistant or -tolerant weeds. Weed Technol 19:924–933CrossRefGoogle Scholar
  55. Ng CH, Wickneswari R, et al (2003) Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Res 43:108–115CrossRefGoogle Scholar
  56. Owen MDK (2000) Current use of transgenic herbicide-resistant soybean and corn in the USA. Crop Protect 19:765–771CrossRefGoogle Scholar
  57. Owen MDK (2001) Importance of weed population shifts and herbicide resistance in the midwest USA corn belt. Br Crop Protect Conf Weeds 2001:407–412Google Scholar
  58. Owen MDK (2007) Weed management in 2008 -- new opportunities, existing issues and anticipated problems. Integr Crop Manage Conf 2007:157–167Google Scholar
  59. Owen MDK (2008a) Glyphosate resistant crops and evolved glyphosate resistant weeds -- the need for stewardship. Int Weed Sci Congr 5:51Google Scholar
  60. Owen MDK (2008b) Weed species shifts in glyphosate-resistant crops. Pest Manage Sci 64:377–387CrossRefGoogle Scholar
  61. Owen MDK (2009) Herbicide-tolerant genetically modified crops: resistance management. In: Ferry N, Gatehouse AMR (eds) Environmental impact of genetically modified crops. CAB International, Wallingford, pp 113–162Google Scholar
  62. Owen M, Boerboom C (2004) National Glyphosate Stewardship Forum. St. Louis, p. 80Google Scholar
  63. Owen MDK, Zelaya IA (2005) Herbicide-resistant crops and weed resistance to herbicides. Pest Manage Sci 61:301–311CrossRefGoogle Scholar
  64. Owen MDK, Boerboom C, et al (2009) Convenience and simplicity? An illusion and a detriment to integrated weed management. Int Integr Pest Manage Symp 6:127Google Scholar
  65. Padgette SR, Delannay X, et al (1995) Development of glyphosate-tolerant crops and perspectives on the potential for weed resistance to glyphosate. Int Symp Weed Crop Resist Herbicides 1995:92Google Scholar
  66. Patzoldt WL, Tranel PJ, et al (2005) A waterhemp (Amaranthus tuberculatus) biotype with multiple resistance across three herbicide sites of action. Weed Sci 53:30–36CrossRefGoogle Scholar
  67. Patzoldt WL, Hager AG, et al (2006) A codon deletion confers resistance to herbicide inhibiting protoporphyrinogen oxidase. Proc Natl Acad Sci USA 103:12329–12334PubMedCrossRefGoogle Scholar
  68. Pineyro-Nelson A, Van Heerwaarden J, et al (2009) Transgenes in Mexican maize: molecular evidence and methodological considerations for GMO detection in landrace populations. Mol Ecol 18:750–761PubMedCrossRefGoogle Scholar
  69. Powell JR, Levy-Booth DJ, et al (2009) Effects of genetically modified, herbicide-tolerant crops and their management on soil food web properties and crop litter decomposition. J Appl Ecol 46:388–396CrossRefGoogle Scholar
  70. Powles SB (2008) Evolution in action: glyphosate-resistant weeds threaten world crops. Outlooks Pest Manage 2008:256–259CrossRefGoogle Scholar
  71. Preston C, Tardif FJ, et al (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pest Biochem Physiol 54:123–134CrossRefGoogle Scholar
  72. Raven PH (2005) Transgenes in Mexican maize: desirability or inevitability? Science 102:13003–13004Google Scholar
  73. Reichman JR, Watrud LS, et al (2006) Establishment of transgenic herbicide-resistant creeping bentgrass (Agrostis stolonifera L.) in nonagronomic habitats. Mol Ecol 15:4243–4255PubMedCrossRefGoogle Scholar
  74. Ryan GF (1970) Resistance fo common groundsel to simazine and atrazine. Weed Sci 18:614–616Google Scholar
  75. Sammons RD, Heering DC, et al (2007) Sustainability and stewardship of glyphosate and glyphosate-resistant crops. Weed Technol 21:347–354CrossRefGoogle Scholar
  76. Scursoni J, Forcella F, et al (2006) Weed diversity and soybean yield with glyphosate management along a north--south transect in the United States. Weed Sci 54:713–719CrossRefGoogle Scholar
  77. Scursoni JA, Forcella F, et al (2007) Weed escapes and delayed emergence in glyphosate-resistant soybean. Crop Protect 26:212–218CrossRefGoogle Scholar
  78. Service RF (2007) A growing threat down on the farm. Science 316:1114–1116PubMedCrossRefGoogle Scholar
  79. Service RF (2007) Glyphosate -- the conservationist's friend? Science 316:1116–1117PubMedCrossRefGoogle Scholar
  80. Shaner DL (2000) The impact of glyphosate-tolerant crops on the use of other herbicides and on resistance management. Pest Manage Sci 56:320–326CrossRefGoogle Scholar
  81. Siehl DL, Castle LA, et al (2005) Evolution of microbial acetyltransferase for modification of glyphosate, a novel tolerance strategy. Pest Manage Sci 61:235–240CrossRefGoogle Scholar
  82. Steckel LE, Sprague CL, et al (2007) Tillage, cropping system, and soil depth effects on common waterhemp (Amaranthus rudis) seed-bank persistence. Weed Sci 55:235–239CrossRefGoogle Scholar
  83. Ulloa SM, Owen MDK (2009) Response of Asiatic dayflower (Commelina communis) to glyphosate and alternatives in soybean. Weed Sci 57:74–80CrossRefGoogle Scholar
  84. Young BG (2006) Changes in herbicide use patterns and production practices resulting from glyphosate-resistant crops. Weed Technol 20:301–307CrossRefGoogle Scholar
  85. Yuan JS, Tranel PJ, et al (2006) Non-target-site herbicide resistance: a family business. Trends Plant Sci 12:6–13PubMedCrossRefGoogle Scholar
  86. Zelaya I, Owen MDK (2000) Differential response of common waterhemp (Amaranthus rudis) to glyphosate in Iowa. Proc Meet Weed Sci Soc Am 40:62–63Google Scholar
  87. Zelaya IA, Owen MDK (2004) Evolved resistance to ALS-inhibiting herbicides in common sunflower (Helianthus annuus), giant ragweed (Ambrosia trifida), and shattercane (Sorghum bicolor) in Iowa. Weed Sci 52:538–548CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Agronomy DepartmentIowa State UniversityAmesUSA

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