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Drought Stress Tolerance

  • Dorothea BartelsEmail author
  • Jonathan Phillips
Chapter
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 64)

Abstract

Drought stress severely limits plant growth and productivity. Traditional plant breeding strategies have made significant contributions to the generation of stress-tolerant plants. The problem in setting-up strategies for generating drought stress tolerant plants is the complex multigenic nature of the tolerance mechanisms. The targeted engineering of metabolic pathways correlated with drought stress has emerged as a promising approach to obtain plants with improved stress tolerance. The transformation of plants using regulatory genes is particularly attractive for producing abiotic stress tolerant plants. The over-expression of regulatory genes can activate the expression of many downstream target genes simultaneously and thus entire pathways can be modified. This review summarizes recent advances in metabolc engineering stress tolerance pathways in crop plants. Emphasis is placed on using regulatory and signalling genes as tools to engineer drought stress.

Keywords

Transgenic Plant Drought Stress Stress Tolerance Drought Tolerance Transgenic Rice 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Work in the laboratory of D.B. was supported by grants from the German Research Council, by the European training network ADONIS, and by an ERA-PG project. D.B. is a member of the European COST action International Network of Plant Abiotic Stress (INPAS).

References

  1. Abebe T, Guenzi AC, Martin B, Cushman JC (2003) Tolerance of mannitol accumulating transgenic wheat to water stress and salinity. Plant Physiol 131:1748–1755PubMedCrossRefGoogle Scholar
  2. Assmann SM, Wang XQ (2001) From milliseconds to millions of years: guard cells and environmental responses. Curr Opin Plant Biol 4:421–428PubMedCrossRefGoogle Scholar
  3. Banno H, Hirano K, Nakamura T, Irie K, Nomoto S, Matsumoto K, Machida Y (1993) NPK1, a tobacco gene that encodes a protein with a domain homologous to yeast BCK1, STE11, and Byr2 protein kinases. Mol Cell Biol 13:4745–4752PubMedGoogle Scholar
  4. Bartels D, Hussain SS (2008) Current status and implications of engineering drought tolerance in plants using transgenic approaches. (CAB reviews 3: perspectives in agriculture, veterinary science, nutrition and natural resources) CAB Rev 3:1–17Google Scholar
  5. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58CrossRefGoogle Scholar
  6. Bohnert HJ, Shen B (1999) Transformation and compatible solutes. Sci Hortic 78:237–260CrossRefGoogle Scholar
  7. Blum A (1988) Plant breeding for stress environments. CRC, Boca RatonGoogle Scholar
  8. Capell T, Bassie L, Christou P (2004) Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proc Natl Acad Sci USA 101:9909–9914PubMedCrossRefGoogle Scholar
  9. CGIAR (2006) Consultative Group on International Agricultural Research (CGIAR) announces intensified research effort to make agriculture more climate resilient. CGIAR News. http://www.cgiar.org/enews/december2006/story_02.html. Accessed 30 Dec 2006
  10. Chen TH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol:5:250–257PubMedCrossRefGoogle Scholar
  11. Chen TH, Murata N (2008) Glycinebetaine: an effective protectant against abiotic stress in plants. Trends Plant Sci 13:499–505PubMedCrossRefGoogle Scholar
  12. Christensen AH, Sharrock RA, Quail PH (1992) Maize polyubiquitin genes -- structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol 18:675–689PubMedCrossRefGoogle Scholar
  13. de Ronde JA, Spreeth MH, Cress WA (2000) Effect of antisense L-Δ1-pyrroline-5-carboxylate reductase transgenic soybean plants subjected to osmotic and drought stress. Plant Growth Regul 32:13–26CrossRefGoogle Scholar
  14. Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223CrossRefGoogle Scholar
  15. Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, Barron C, Brady SM, Schiefelbein J, Benfey PN (2008) Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320:942–945PubMedCrossRefGoogle Scholar
  16. FAO (2006) The state of food insecurity in the world. FAO, RomeGoogle Scholar
  17. Fitzgerald TL, Waters DLE, Henry RJ (2008) Betaine aldehyde dehydrogenase in plants. Plant Biol 11:119–130CrossRefGoogle Scholar
  18. Garg AK, Kim J-K, Owens TG, Ranwala AP, Choi YD, Kochian LV, Wu RJ (2002) Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci USA 99:15898–15903PubMedCrossRefGoogle Scholar
  19. Goodijn OJM, van Dun K (1999) Trehalose metabolism in plants. Trends Plant Sci 4:315–319CrossRefGoogle Scholar
  20. Hasegawa PM, Bressan R, Zhu J-K, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499PubMedCrossRefGoogle Scholar
  21. Hsieh TH, Lee JT, Charng YY, Chan MT (2002) Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. Plant Physiol 130:618–626PubMedCrossRefGoogle Scholar
  22. Hu HH, Dai MQ, Yao JL, Xiao BZ, Li XH, Zhang QF, Xiong LZ (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA 103:12987–12992PubMedCrossRefGoogle Scholar
  23. Hu HH, You J, Fang YJ, Zhu XY, Qi ZY, Xiong LZ (2008) Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Mol Biol 67:169–181PubMedCrossRefGoogle Scholar
  24. Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Biol 47:377–403CrossRefGoogle Scholar
  25. Jaglo KR, Kleff S, Amundsen KL, Zhang X, Haake V, Zhang JZ, Deits T, Thomashow MF (2001) Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol 127:910–917PubMedCrossRefGoogle Scholar
  26. Jang IC, Oh SJ, Seo JS, Choi WB, Song SI, Kim CH, Kim YS, Seo HS, Choi YD, Nahm BH, Kim JK (2003) Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphate in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol 131:516–524PubMedCrossRefGoogle Scholar
  27. Karaba A, Dixit S, Greco R, Aharoni A, Trijatmiko KR, Marsch-Martinez N, Krishnan A, Nataraja KN, Udayakumar M, Pereira A (2007) Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene. Proc Natl Acad Sci USA 104:15270–15275PubMedCrossRefGoogle Scholar
  28. Kasuga M, Liu Q, Miura S, Yamaguchi S, Shinozaki K (1999) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17:287–291PubMedCrossRefGoogle Scholar
  29. Kishor PBK, Hong Z, Miao GH, Hu CAA, Verma DPS (1995) Overexpression of delta 1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394PubMedGoogle Scholar
  30. Kovtun Y, Chiu W-L, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated MAPK cascade in plants. Proc Natl Acad Sci USA 97:2940–2945PubMedCrossRefGoogle Scholar
  31. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406PubMedGoogle Scholar
  32. Mantovani R (1999) The molecular biology of the CCAAT-binding factor NF-Y. Gene 239:15–27PubMedCrossRefGoogle Scholar
  33. Mohanty A, Kathuria H, Ferjani A, Sakamoto A, Mohanty P, Murata N,Tyagi AK (2002) Transgenics of an elite indica rice variety Pusa Basmati 1 harbouring the codA gene are highly tolerant to salt stress. Theor Appl Gen:106:51–57Google Scholar
  34. Nakano T, Suzuki K, Fujimura T, Shinshi H. (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140:411–432PubMedCrossRefGoogle Scholar
  35. Nakashima K, Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K (1997) A nuclear gene encoding a chloroplast-targeted Clp protease regulatory subunit homolog is not only induced by water stress but also developmentally up-regulated during senescence in Arabidopsis thaliana. Plant J 12:851–861PubMedCrossRefGoogle Scholar
  36. Nanjo T, Kobayashi M, Yoshiba Y, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K (1999) Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana. FEBS Lett 461:205–210PubMedCrossRefGoogle Scholar
  37. Nelson DE, Repetti PP, Adams TR, Creelman RA, Wu J, Warner DC, Anstrom DC, Bensen RJ, Castiglioni PP, Donnarummo MG, Hinchey BS, Kumimoto RW, Maszle DR, Canales RD, Krolikowski KA, Dotson SB, Gutterson N, Ratcliffe OJ, Heard JE (2007) Heard plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA 104:16450–16455PubMedCrossRefGoogle Scholar
  38. Nuccio ML, Russell BL, Nolte KD, Rathinasabapathi B, Gage DA, Hanson AD (1998) The endogenous choline supply limits glycine betaine synthesis in transgenic tobacco expressing choline monooxygenase. Plant J 16:487–496PubMedCrossRefGoogle Scholar
  39. Odell JT, Nagy F, Chua NH (1985) Identification of DNA-sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313:810–812PubMedCrossRefGoogle Scholar
  40. Oh SJ, Song SI, Kim YS, Jang HJ, Kim SY, Kim M, Kim YK, Nahm BH, Kim JK (2005) Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol 138:341–351PubMedCrossRefGoogle Scholar
  41. Oh SJ, Kwon CW, Choi DW, Song SI, Kim JK (2007) Expression of barley HvCBF4 enhances tolerance to abiotic stress in transgenic rice. Plant Biotechnol J 5:646–656PubMedCrossRefGoogle Scholar
  42. Olsen AN, Ernst HA, Leggio LL, Skriver K (2005) NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci 10:79–87PubMedCrossRefGoogle Scholar
  43. Park E-J, Jeknic Z, Pino M-T, Murata N, Chen TH-H (2007) Glycinebetaine accumulation is more effective in chloroplasts than in the cytosol for protecting transgenic tomato plants against abiotic stress. Plant Cell Environ:30:994–1005PubMedCrossRefGoogle Scholar
  44. Paul M (2007) Trehalose 6-phosphate. Curr Opin Plant Biol 10:303–309PubMedCrossRefGoogle Scholar
  45. Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Shinozaki K, Hoisington D (2004) Stress induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47:493–500PubMedCrossRefGoogle Scholar
  46. Pilon-Smits E, Terry N, Sears T, van Dunn K (1999) Enhanced drought resistance in fructan producing sugar beet. Plant Physiol Biochem 37:313–317CrossRefGoogle Scholar
  47. Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110PubMedCrossRefGoogle Scholar
  48. Roosens NH, Al Bitar F, Loenders K, Angenon G, Jacobs M (2002) Oberexpression of ornithine-δ-aminotransferase increases pro biosynthesis and confers osmotolerance in transgenic plants. Mol Breed 9:73–80CrossRefGoogle Scholar
  49. Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K (2000) Overexpression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23:319–327PubMedCrossRefGoogle Scholar
  50. Sakamoto A, Alia A, Murata N (1998) Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold. Plant Mol Biol 38:1011–1019PubMedCrossRefGoogle Scholar
  51. Sanders D, Brownlee C, Harper JF (1999) Communicating with calcium. Plant Cell 11:691–706PubMedGoogle Scholar
  52. Sawahel WA, Hassan AH (2002) Generation of transgenic wheat plants producing high levels of osmoprotectant proline. Biotechnol Lett 24:721–725CrossRefGoogle Scholar
  53. Schroeder JI, Allen GJ, Hugouvieux V, Kwak JM, Waner D (2001) Guard cell signal transduction. Annu Rev Plant Physiol Plant Mol Biol 52:627–658PubMedCrossRefGoogle Scholar
  54. Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of ca. 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292PubMedCrossRefGoogle Scholar
  55. Serraaj R, Sinclair TR (2002) Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant Cell Environ 25:333–341CrossRefGoogle Scholar
  56. Shirawasa K, Takabe T, Takabe T, Kishitani S (2006) Accumulation of glycinebetaine in rice plants that overexpress choline monooxygenase from spinach and evaluation of their tolerance to abiotic stress. Ann Bot 98:565–571CrossRefGoogle Scholar
  57. Shou H, Bordallo P, Wang K (2004) Expression of the Nicotiana protein kinase NPK1 enhanced drought tolerance in transgenic maize. J Exp Bot 55:1013–1019PubMedCrossRefGoogle Scholar
  58. Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA 94:1035–1040PubMedCrossRefGoogle Scholar
  59. Sunkar R, Bartels D, Kirch HH (2003) Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. Plant J 35:452–464PubMedCrossRefGoogle Scholar
  60. Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498PubMedCrossRefGoogle Scholar
  61. Tuberosa R, Salvi S (2006) Genomics-based approaches to improve drought tolerance of crops. Trends Plant Sci 11:405–412PubMedCrossRefGoogle Scholar
  62. Wang QY, Guan YC, Wu YR, Chen HL, Chen F, Chu CC (2008) Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice. Plant Mol Biol 67:589–602PubMedCrossRefGoogle Scholar
  63. Xu D, Duan X, Wang B, Hong B, Ho THD, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110:249–257PubMedGoogle Scholar
  64. Yamaguchi-Shinozaki K, Shinozaki K (1994) A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6:251–264PubMedGoogle Scholar
  65. Yan J, He C, Wang J, Mao Z, Holaday SA, Allen RD, Zhang H (2004) Overexpression of the Arabidopsis 14-3-3 protein GF14λ in cotton leads to a stay-green phenotype and improves stress tolerance under moderate drought conditions. Plant Cell Physiol 45:1007–1014PubMedCrossRefGoogle Scholar
  66. Yu H, Chen X, Hong Y-Y, Wang Y, Xu P, Ke S-D, Liu H-Y, Zhu J-K, Oliver DJ, Xiang C-B (2008) Activated expression of an Arabidopsis HD-START protein confers drought tolerance with improved root system and reduced stomatal density. Plant Cell 20:1134–1151PubMedCrossRefGoogle Scholar
  67. Zhu B, Su J, Chang M, Verma DPS, Fan Y-L, Wu R (1998) Overexpression of a D1-pyrroline-5-carboxylate synthetase gene and analysis of tolerance to water- and salt-stress in transgenic rice. Plant Sci 139:41–48CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Institute of Molecular Biology and Biotechnology of Plants (IMBIO)University of BonnBonnGermany

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