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Sugarcane

  • Fredy AltpeterEmail author
  • Hesham Oraby
Chapter
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 64)

Abstract

Sugarcane (Saccharum sp. hybrids) is a highly productive C 4 grass used as the main source of sugar and more recently to produce ethanol, a renewable transportation fuel. There is increased interest in this crop due to the impending need to decrease the dependency on fossil fuels. This chapters discusses tissue culture and transgenic approaches aiming at incorporation of herbicide resistance , abiotic and biotic stress tolerance, metabolic engineering and the production of value added products in sugarcane. Future developments are expected to lead to the commercial release of transgenic sugarcane and may include its development into a biofactory for high-value products.

Keywords

Somatic Embryo Embryogenic Callus Immature Leaf Immature Inflorescence Galanthus Nivalis Agglutinin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Altpeter F, Vasil V, Srivastava V, Stoger E, Vasil IK (1996) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep 16:12–17Google Scholar
  2. Altpeter F, Baisakh N, Beachy R, Bock R, Capell T, Christou P, Daniell H, Datta K, Datta S, Dix PJ, Fauquet C, Huang N, Kohli A, Mooibroek H, Nicholson L, Nguyen TT, Nugent G, Raemakers K, Romano A, Somers DA, Stoger E, Taylor N, Visser R (2005) Particle bombardment and the genetic enhancement of crops: myths and realities. Mol Breed 15:305–327Google Scholar
  3. Allsopp PG, Suasa-ard W (2000) Sugarcane pest management strategies in the new millennium. Proc Int Soc Sugar Cane Technol Sugarcane Entomol Workshop 2000:4Google Scholar
  4. Arencibia A, Molina P, Gutierrez C, Fuentes A, Greenidge V, Menendez E, De la Riva G, Selman G (1992) Regeneration of transgenic sugarcane (Saccharum officinarum L.) plants from intact meristematic tissues transformed by electroporation. Biotecnol Apl 9:156–165Google Scholar
  5. Arencibia A, Molina P, De la Riva G, Selman-Houssein G (1995) Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation. Plant Cell Rep 14:305–309Google Scholar
  6. Arencibia A, Vazquez RI, Prieto D, Tellez P, Carmona ER, Coego A, Hernandez L, De la Riva GA, Selman-Housein G (1997) Transgenic sugarcane plants resistant to stem borer attack. Mol Breed 3:247–255Google Scholar
  7. Arencibia AD, Carmona ER, Tellez P, Chan M, Yu S, Trujillo LE, Oramas P (1998) An efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens. Transgenic Res 7:213–222Google Scholar
  8. Arencibia A, Carmona E, Cornide MT, Castiglione S, O'Relly J, Cinea A, Oramas P, Sala F (1999) Somaclonal variation in insect resistant transgenic sugarcane (Saccharum hybrid) plants produced by cell electroporation. Transgenic Res 8:349–360Google Scholar
  9. Barba R, Nickell LG (1969) Nutrition and organ differentiation in tissue culture of sugarcane – a monocotyledon. Planta 89:299–302Google Scholar
  10. Baulcombe DC (1996) Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8:1833–1844PubMedGoogle Scholar
  11. Birch RG (2007) Metabolic engineering in sugarcane: assisting the transition to a bio-based economy. In: Verpoorte R, et al (eds) Applications of plant metabolic engineering. Springer, Heidelberg, pp 249–281Google Scholar
  12. Bonnett GD, Nowak E, Olivares-Villegas JJ, Berding N, Morgan T, Aitken KS (2008) Identifying the risks of transgene escape from sugarcane crops to related species, with particular reference to Saccharum spontaneum in Australia. Trop Plant Biol 1:58–71Google Scholar
  13. Botha FC, Groenewald JH (2001a) Manipulating sucrose metabolism with a single enzyme: pyrophosphate-dependent phosphofructokinase (PFP). Proc S Afr Sugar Technol Assoc 75:101–103Google Scholar
  14. Botha FC, Groenewald JH (2001b) Down regulating pyrophosphate-dependent phosphofructokinase (PFP) in sugarcane. Proc Int Soc Sugar Cane Technol 24:592–594Google Scholar
  15. Botha FC, Groenewald JH (2001c) Method for regulating or manipulating sucrose content and metabolism in sugar storing plants, e.g. increasing sucrose content, by regulating activity of pyrophosphate-dependent phosphofructokinase enzyme in plants. S Afr Patent Application ZA200101047-A 25Jul2001Google Scholar
  16. Bower R, Birch RG (1992) Transgenic sugarcane plants via microprojectile bombardment. Plant J 2:409–416Google Scholar
  17. Brandes EW (1958) Origin, classification and characteristics. In: Artschwager E, Brandes EW (ed) Sugarcane (S. officinarum L.). USDA agricultural handbook. USDA, Washington, D.C., pp 1–35Google Scholar
  18. Burner DM, Grisham MP (1995) Induction and stability of phenotypic variation in sugarcane as affected by propgation procedure. Crop Sci 35:875–878Google Scholar
  19. Burner DM, Legendre BL (1994) Cytogenetic and fertility characteristics of elite sugarcane clones. Sugar Cane 1:6–10Google Scholar
  20. Campbell MA, Fitzgerald HA, Ronald PC (2002) Engineering pathogen resistance in crop plants. Transgenic Res 11:599–613PubMedGoogle Scholar
  21. Chen F, Dixon R (2007) Lignin modification improves fermentable sugar yields for biofuel production. Nat Biotechnol 25:759–761PubMedGoogle Scholar
  22. Chen WC, Gartland KMA, Davey MR, Sotak R, Gartland JS, Mulligan BJ, Power JB, Cocking EC (1987) Transformation of sugarcane protoplasts by direct uptake of a selectable chimeric gene. Plant Cell Rep 6:297–301Google Scholar
  23. Chengalrayan K, Gallo-Meagher M (2001) Effect of various growth regulators on shoot regeneration of sugarcane. In Vitro Cell Dev Biol Plant 37:434–439Google Scholar
  24. Chong BF, Bonnett GD, Glassop D, Shea MG, Brumbley SM (2007) Growth and metabolism in sugarcane altered by the creation of a new hexose-phosphate sink. Plant Biotechnol J 5:240–253PubMedGoogle Scholar
  25. Chowdhury MKU, Vasil IK (1992) Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts. Plant Cell Rep 11:494–498Google Scholar
  26. Cuadrado A, Acevedo R, Moreno Dı'az de la Espina S, Jouve N, de la Torre C (2004) Genome remodeling in three modern S. officinarum x S. spontaneum sugarcane cultivars. J Exp Biol 55:847–854Google Scholar
  27. Daniels J, Roach BT (1987) Taxonomy and evolution. In: Heinz DJ (ed) Sugarcane improvement through breeding, vol 11. Elsevier, Amsterdam, etherlands, pp 7–84Google Scholar
  28. Denchev PD, Songstad DD, McDaniel JK, Conger BV (1997) Transgenic orchardgrass (Dactylis glomerata) plants by direct embryogenesis from microprojectile bombarded leaf cells. Plant Cell Rep 16:813–819Google Scholar
  29. Desai NS, Suprasanna P, Bapat VA (2004) Simple and reproducible protocol for direct somatic embryogenesis from cultured immature inflorescence segments of sugarcane (Saccharum spp.). Curr Sci 87:764–768Google Scholar
  30. D'Hont A, Grivet L, Feldmann P, Rao S, Berding N, Glaszmann JC (1996) Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Mol Gen Genet 250:405–413PubMedGoogle Scholar
  31. Drennan PM, Smith MT, Goldsworthy D, van Staden J (1993) The occurrence of trehalose in the leaves of the desiccation-tolerant angiosperm Myrothamnus flabellifolius. J Plant Physiol 142:493–496Google Scholar
  32. Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33:751–763PubMedGoogle Scholar
  33. Elliott AR, Campbell JA, Brettell RIS,Grof CPL (1998) Agrobacterium-mediated transformation of sugarcane using GFP as a screenable marker. Aust J Plant Physiol 25:739–743Google Scholar
  34. Enríquez-Obregón GA, Vázquez-Padrón RI, Prieto-Samsonov DL, De la Riva GA and Selman-Housein G (1998) Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation. Planta 206:1432–2048Google Scholar
  35. Falco MC, Neto AT, Ulian EC (2000) Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane. Plant Cell Rep 19:1188–1194Google Scholar
  36. Falco MC, Silva-Filho MC (2003) Expression of soybean proteinase inhibitors in transgenic sugarcane plants: effects on natural defense against D. saccharalis. Plant Phys Biochem 41:761–766Google Scholar
  37. Ferreira SJ, Kossmann J, Lloyd JR, Groenewald J (2008) The reduction of starch accumulation in transgenic sugarcane cell suspension culture lines. Biotechnol J 3:1398–1406. doi:10.1002/biot.200800106PubMedGoogle Scholar
  38. Gallo-Meagher M, Irvine JE (1996) Herbicide resistant transgenic sugarcane plants containing the bar gene. Crop Sci 36:1367–1374Google Scholar
  39. Gallo-Meagher M, English RG, Abouzid A (2000) Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus. In Vitro Cell Dev Biol Plant 36:37–40Google Scholar
  40. Garcia R, Cidade D, Castellar A, Lips A, Magioli C, Callado C, Mansur E (2007) In vitro morphogenesis patterns from shoot apices of sugarcane are determined by light and type of growth regulator. Plant Cell Tiss Organ Cult 90:181–190Google Scholar
  41. Gilbert RA, Gallo-Meagher M, Comstock JC, Miller JD, Jain M, Abouzid A (2005) Agronomic evaluation of sugarcane lines transformed for resistance to Sugarcane mosaic virus strain E. Crop Sci 45:2060–2067Google Scholar
  42. Gill R, Malhotra PK, Gosal SS (2006) Direct plant regeneration from cultured young leaf segments of sugarcane. Plant Cell Tiss Org Cult 84:227–231Google Scholar
  43. Grivet L, D'Hont A, Roques D, Feldmann P, Lanaud C, Glaszmann JC (1996) RFLP mapping in cultivated sugarcane (Saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. Genetics 142:987–1000PubMedGoogle Scholar
  44. Groenewald J, Botha FC (2008) Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodes. Transgenic Res 17:85–92PubMedGoogle Scholar
  45. Grof CL, Campbell JA (2001) Sugarcane sucrose metabolism: scope for molecular manipulation. Aust J Plant Physiol 28:1–12Google Scholar
  46. Hare P, Chua NH (2002) Excision of selectable marker genes from transgenic plants. Nat Biotechnol 20:575–580PubMedGoogle Scholar
  47. Heinz DJ, Mee GWP (1969) Plant differentiation from callus tissue of Saccharum species. Crop Sci 9:346–348Google Scholar
  48. Hendre RR, Iyer RS, Kotwal M, Khuspe SS, Mascarenhas AF (1983) Rapid multiplication of sugarcane by tissue culture. Sugarcane 1:5–8Google Scholar
  49. Ho WJ, Vasil IK (1983a) Somatic embryogenesis in sugarcane (Saccharum officinarum L.). 1. The morphology and ontogeny of somatic embryos. Protoplasma 118:169–180Google Scholar
  50. Ho WJ, Vasil IK (1983b) Somatic embryogenesis in sugarcane (Saccharum officinarum L.): growth and plant regeneration from embryogenic cell suspension cultures. Ann Bot 51:719–726Google Scholar
  51. Howard JA, Hood E (2005) Bioindustrial and biopharmaceutical products produced in plants. Adv Agron 85:91–124Google Scholar
  52. Ingelbrecht IL, Irvine JE, Mirkov TE (1999) Posttranscriptional gene silencing in transgenic sugarcane. Dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. Plant Physiol 119:1187–1197PubMedGoogle Scholar
  53. Irvine JE, Gallo-Meagher M, Mirkov ET (1996) Genetic engineering for herbicide resistance in three sugarcane varieties. Proc Inter Am Sugar Cane Sem 1:166–171Google Scholar
  54. Jackson PA (2005) Breeding for improved sugar content in sugarcane. Field Crops Res 92:277–290Google Scholar
  55. Jain M, Chengalrayan K, Abouzid A, Gallo M (2007) Prospecting the utility of a PMI/mannose selection system for the recovery of transgenic sugarcane plants. Plant Cell Rep 26:581–590PubMedGoogle Scholar
  56. James VA, Neibaur I, Altpeter F (2008) Stress inducible expression of the DREB1A transcription factor from xeric, Hordeum spontaneum enhances abiotic stress tolerance in turf and forage grass (Paspalum notatum Flugge). Transgenic Res 17:93–104PubMedGoogle Scholar
  57. Joyce PA, McQualter RB, Handley JA, Dale JL, Harding RM, Smith GR (1998) Transgenic sugarcane resistant to sugarcane mosaic virus. Proc Aust Soc Sugarcane Technol 20:204–210Google Scholar
  58. Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17:287–291PubMedGoogle Scholar
  59. Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235–246PubMedGoogle Scholar
  60. Lakshmanan P (2006) Somatic embryogenesis in sugarcane – an addendum to the invited review ‘sugarcane biotechnology: the challenges and opportunities,’ In Vitro Cell. Dev. Biol. Plant 41(4):345–363; 2005. In Vitro Cell Dev Biol Plant 42:201–205Google Scholar
  61. Lakshmanan P, Geijskes RJ, Aitken KS, Grof CPL, Bonnett GD, Smith GR (2005) Sugarcane biotechnology: the challenges and opportunities. In Vitro Cell Dev Biol Plant 41:345–363Google Scholar
  62. Lakshmanan P, Geijskes RJ, Wang LF, Elliott A, Grof CPL, Berding N, Smith GR (2006) Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture. Plant Cell Rep 25:1007–1015PubMedGoogle Scholar
  63. Leal MR, Maribona RH, Ruiz S, Korneva E, Canales TD, Dinkova F, Izquierdo, Coto O, Rizo D (1996) Somaclonal variation as a source of resistance to eyespot disease of sugarcane. Plant Breed 115:37–42Google Scholar
  64. Lee TSG (1987) Micropropagation of sugarcane (Saccharum spp.). Plant Cell Tiss Organ Cult 10:47–55Google Scholar
  65. Leibbrandt NB, Snyman SJ (2003) stability of gene expression and agronomic performance of a transgenic herbicide-resistant sugarcane line in South Africa. Crop Sci 43:671–677Google Scholar
  66. Liu MC (1990) Selection of agronomically useful mutants through plant cell culture systems. Taiwan Sugar 37:81–13Google Scholar
  67. Liu MC (1993) Factors affecting induction, somatic embryogenesis and plant regeneration of callus from cultured immature inflorescences of sugarcane. J Plant Physiol 141:714–720Google Scholar
  68. Ma H, Albert HH, Paull R, Moore PH (2000) Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells. Aust J Plant Physiol 27:1021–1030Google Scholar
  69. Ma JKC, Chikwarnba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals – the road forward. Trends Plant Sci 10:580–585PubMedGoogle Scholar
  70. Manickavasagam M, Ganapathi A, Anbazhagan VR, Sudhakar B, Selvaraj N, Vasudevan A, Kasthurirengan S (2004) Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using axillary buds. Plant Cell Rep 23:134–143PubMedGoogle Scholar
  71. McQualter RB, Dale JL, Harding RM, McMahon JA, Smith GR (2004) Production and evaluation of transgenic sugarcane containing a Fiji disease virus (FDV) genome segment S9-derived synthetic resistance gene. Aust J Agric Res 55:139–145Google Scholar
  72. McQualter RB, Fong Chong B, O'Shea M, Meyer K, van Dyk DE, Viitanen PV, Brumbley SM (2005) Initial evaluation of sugarcane as a production platform for a p-hydroxybenzoic acid. Plant Biotechnol J 2:1–13Google Scholar
  73. Menendez R, Fernandez SI, Del-Rio A, Gonzalez RM, Fraga V, Amor AM, Mas RM (1994) Policosanol inhibits cholesterol biosynthesis and enhances low density lipoprotein processing in cultured human fibroblasts. Biol Res 27:199–203PubMedGoogle Scholar
  74. Ming R, Moore PH, Wu KK, Hont AD, Glaszmann JC, Tew TL, Mirkov TE, da Silva J, Jifon J, Rai M, Schnell RJ, Brumbley SM, Lakshmanan P, Comstock JC, Paterson AH (2006) Sugarcane improvement through breeding and biotechnology. Plant Breed Rev 27:15–118Google Scholar
  75. Molinaria HBC, Marura CJ, Darosb E, Camposa MK, Carvalhoa JF, Filhob JC, Pereirac LF, Vieiraa LG (2007) Evaluation of the stress-inducible production of proline in transgenic sugarcane: osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol Plant 130:218–229Google Scholar
  76. Nutt KA, Allsopp PG, McGhie TK, Shepherd KM, Joyce PA, Taylor GO, McQualter RB, Smith GR (1999) Transgenic sugarcane with increased resistance to canegrubs. Proc Aust Soc Sugarcane Technol 21:171–176Google Scholar
  77. Opabode TJ (2006) Agrobacterium-mediated transformation of plants: emerging factors that influence efficiency. Biotechnol Mol Biol Rev 1:12–20Google Scholar
  78. Oraby H, Venkatesh B, Dale B, Ahmad R, Ransom C, Oehmke J, Sticklen M (2007) Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol. Transgenic Res 16:739–749PubMedGoogle Scholar
  79. Petrasovits LA, Purnell MP, Nielsen LK, Brumbley SM (2007) Production of polyhydroxybutyrate in sugarcane. Plant Biotechnol J 5:162–172PubMedGoogle Scholar
  80. Purnell MP, Petrasovits LA, Nielsen LK, Brumbley SM (2007) Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane. Plant Biotechnol 5:173–184Google Scholar
  81. Purseglove JW (1972) Tropical crops: monocotyledons. Longman, London,Google Scholar
  82. Rathus C, Birch RG (1992) Stable transformation of callus from electroporated sugarcane protoplasts. Plant Sci 82:81–89Google Scholar
  83. Rein PW (2007) Prospects for the conversion of a sugar mill into a biorefinery. Proc Int Soc Sugar Cane Technol 26:44–60Google Scholar
  84. Rohwer JM, Uys L, Meyer KL, Botha FC, Hofmeyr JH (2007) Plant-scale kinetic and structural modeling of sucrose accumulation in sugarcane. Proc Int Conf Syst Biol 8:84Google Scholar
  85. Rott P, Ael M, Soupa D, Feldmann P, Letourmy P (1994) Population dynamics of Xanthomonas albilineans in sugarcane plants as determined with an antibiotic-resistant mutant. Plant Dis 78:241–247Google Scholar
  86. Rott P, Bailey RA, Comstock JC, Croft BJ, Saumtally AS (2000) A guide to sugarcane diseases. CIRAD and ISSCT, MontpellierGoogle Scholar
  87. Sanford JC, Johnstone SA (1985) The concept of parasite-derived resistance: deriving resistance genes from the parasite's own genome. J Theor Biol 115:395–405Google Scholar
  88. Sauvaire D, Galzy R (1978) Multiplication vegetative de canne a sucre (Saccharum sp.) par bouturage in vitro. CR Acad Sci Paris Ser D 1978:467–470Google Scholar
  89. Scheller J, Conrad U (2005) Plant-based material, protein and biodegradable plastic. Curr Opin Plant Biol 8:188–196PubMedGoogle Scholar
  90. Setamou M, Bernal JS, Legaspi JC, Mirkov TE, Legaspi BC (2002) Evaluation of lectin-expressing transgenic sugarcane against stalkborers (Lepidoptera: Pyralidae): effects on life history parameters. J Econ Entomol 95:469–477PubMedGoogle Scholar
  91. Singh G, Sandhu SK, Meeta M, Singh K, Gill R, SS Gosal (2008) In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane. Euphytica 160:35–47Google Scholar
  92. Slater S, Mitsky TA, et al (1999) Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production. Nat Biotechnol 17:1011–1016PubMedGoogle Scholar
  93. Smith AM (2008) Prospects for increasing starch and sucrose yields for bioethanol production. Plant J 54:546–558PubMedGoogle Scholar
  94. Snell KD, Peoples OP (2002) Polyhydroxyalkonate polymers and their production in transgenic plants. Metabol Eng 4:29–40Google Scholar
  95. Snyman SJ (2004) Sugarcane transformation. In: Curtis IS (ed) Transgenic crops of the world – essential protocols. Kluwer, Amsterdam, pp 103–114Google Scholar
  96. Snyman SJ, Huckett BI, Watt MP, Botha FC (2001) A comparison of direct and indirect somatic morphogenesis for the production of transgenic sugarcane (Saccharum spp. hybrids). Acta Hort 560:105–108Google Scholar
  97. Snyman SJ, Meyer GM, Richards JM, Haricharan N, Ramgareeb S, Huckett BI (2006) Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency. Plant Cell Rep 25:1016–1023PubMedGoogle Scholar
  98. Sood N, Gupta PK, Srivastava RK, Gosal SS (2006) Comparative studies on field performance of micropropagated and conventionally propagated sugarcane plants. Plant Tiss Cult Biotechnol 16:25–29Google Scholar
  99. Sreenivasan TV, Ahloowalia BS, Heinz DJ (1987) Cytogenetics. In: Heinz DJ (ed) Sugarcane improvement through breeding, Elsevier, Amsterdam, pp 211–253Google Scholar
  100. Sticklen M (2006) Plant genetic engineering to improve biomass characteristics for biofuels. Curr Opin Biotechnol 17:315–319PubMedGoogle Scholar
  101. Sugiyama M (1999) Organogenesis in vitro. Curr Opin Plant Biol 2:61–64PubMedGoogle Scholar
  102. Tester M, Bacic A (2005) Abiotic stress tolerance in grasses. From model plants to crop plants. Plant Physiol 137:791–793PubMedGoogle Scholar
  103. Tzfira T, Citovsky V (2006) Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Current Opin Biotechnol 17:147–154Google Scholar
  104. Vasil IK, Vasil V (1994) In vitro culture of cereals and grasses. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Springer, Heidelberg, pp 293–312Google Scholar
  105. Vickers JE, Grof CPL, Bonnett GD, Jackson PA, Knight DP, Roberts SE, Robinson SP (2005) Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugar. Crop Sci 45:354–362Google Scholar
  106. Vinocur B, Altman A (2005) Recent advances in engineering plant tolerance to abiotic stress:achievements and limitations. Curr Opin Biotechnol 16:123–132PubMedGoogle Scholar
  107. Wang ML, Goldstein C, Su W, Moore PH, Albert HH (2005) Production of biologically active GM-CSF in sugarcane: a secure biofactory. Transgenic Res 14:167–178PubMedGoogle Scholar
  108. Watt DA, McCormick AJ, Govender C, Carson DL, Cramer MD, Huckett BI, Botha FC (2005) Increasing the utility of genomics in unraveling sucrose accumulation. Field Crops Res 92:149–158Google Scholar
  109. Weng LX, Deng H, Xu JL, Li Q, Wang LH, Zide J, Hai BZ, Qiwei L, Zhang L (2006) Regeneration of sugarcane elite breeding lines and engineering of stem borer resistance. Pest Manage Sci 62:178–187Google Scholar
  110. Wu L, Birch RG (2007) Doubled sugar content in sugarcane plants modified to produce a sucrose isomer. Plant Biotechnol J 5:109–117PubMedGoogle Scholar
  111. Wu Y, Zhou H, Que YX, Chen RK, Zhang MQ (2008) Cloning and identification of promoter Prd29A and its application in sugarcane drought resistance. Sugar Technol 10:36–41Google Scholar
  112. Zambrano AY, Demey JR, González V (2003) In vitro selection of a glyphosate-tolerant sugarcane cellular line. Plant Mol Biol Rep 21:365–373Google Scholar
  113. Zhang L, Xu J, Birch RG (1999) Engineered detoxification confers resistance against a pathogenic bacterium. Nat Biotechnol 17:1021–1024PubMedGoogle Scholar
  114. Zhang SZ, Yang BP, Feng CL, Chen RK, Luo JP, Cai WW, Liu FH (2006) Expression of the Grifola frondosa trehalose synthase gene and improvement of drought-tolerance in sugarcane (Saccharum officinarum L.). J Integr Plant Biol 48:453–459Google Scholar
  115. Zhangsun D, Luo S, Chen R, Tang K (2008) Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane. Biol Bratisl 62:386–393Google Scholar

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© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Agronomy Department, Plant Molecular Biology Program, Genetics InstituteUniversity of Florida – IFASGainesvilleUSA
  2. 2.Agronomy Department, Plant Breeding ProgramCollege of Agriculture, Zagazig UniversityZagazigEgypt

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