Abstract
Plant research has profited enormously from the options provided by genetic engineering methods. Overexpression of genes from the nucleus or their targeted knock-down by RNA interference has enabled the dissection of individual functions of genes or networks, boosting our understanding of plant genetics and physiology. However, a fraction of the plants genome is still encoded in their organelles, namely mitochondria and plastids. Genetic engineering of the plastid genome became feasible in 1990 and since then it has enabled numerous studies on plastid-encoded gene functions and also on biotechnological approaches for recombinant protein production. Although, plastid transformation differs largely from nuclear transformation regarding vector requirements, DNA delivery, and host range. Our intention is to give an overview about the techniques applied, the options, and the drawbacks of plastid transformation.
References
Ahlert D, Ruf S, Bock R (2003) Plastid protein synthesis is required for plant development in tobacco. Proc Natl Acad Sci USA 100:15730–15735
Birch-Machin I, Newell CA, Hibberd JM, Gray C (2004) Accumulation of rotavirus VP6 protein in chloroplasts of transplastomic tobacco is limited by protein stability. Plant Biotech J 2:261–270
Bock R, Maliga P (1995) In vivo testing of a tobacco plastid DNA segment for guide RNA function in psbL editing. Mol Gen Genet 247:439–443
Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, et al. (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240:1534–1538
Carrer H, Maliga P (1995) Targeted Insertion of Foreign Genes into the Tobacco Plastid Genome without Physical Linkage to the Selectable Marker Gene. Nat Biotechnol 13:791–794
Carrer H, Hockenberry TN, Svab Z, Maliga P (1993) Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol Gen Genet 241:49–56
Cerutti H, Osman M, Grandoni P, Jagendorf AT (1992) A homolog of Escherichia coli RecA protein in plastids of higher plants. Proc Natl Acad Sci USA 89:8068–8072
Corneille S, Lutz K, Svab Z, Maliga P (2001) Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant J 27:171–178
Daniell H, Muthukumar B, Lee SB (2001) Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection. Curr Genet 39:109–116
De Cosa B, Moar W, Lee SB, Miller M, Daniell H (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19:71–74
De Marchis F, Wang Y, Stevanato P, Arcioni S, Bellucci M (2009) Genetic transformation of the sugar beet plastome. Transgenic Res 18:17–30
Dufourmantel N, Pelissier B, Garcon F, Peltier G, Ferullo JM, Tissot G (2004) Generation of fertile transplastomic soybean. Plant Mol Biol 55:479–489
Eibl C, Zou Z, Beck a, Kim M, Mullet J, Koop HU (1999) In vivo analysis of plastid psbA, rbcL and rpl32 UTR elements by chloroplast transformation: tobacco plastid gene expression is controlled by modulation of transcript levels and translation efficiency. Plant J 19:333–345
Golds T, Maliga P, Koop H-U (1993) Stable plastid transformation in PEG-treated protoplasts of Nicotiana tabacum. Bio/Technology 11:95–97
Gould SB, Waller RF, McFadden GI (2008) Plastid evolution. Annu Rev Plant Biol 59:491–517
Hager M, Bock R (2000) Enslaved bacteria as new hope for plant biotechnologists. Appl Microbiol Biotechnol 54:302–310
Hajdukiewicz PT, Gilbertson L, Staub JM (2001) Multiple pathways for Cre/lox-mediated recombination in plastids. Plant J 27:161–170
Hennig A, Bonfig K, Roitsch T, Warzecha H (2007) Expression of the recombinant bacterial outer surface protein A in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis. FEBS J 274:5749–5758
Hou B-K, Zhou Y-H, Wan L-H, Zhang Z-L, Shen G-F, Chen Z-H, Hu Z-M (2003) Chloroplast Transformation in Oilseed Rape. Transgenic Res 12:111–114
Huang FC, Klaus SM, Herz S, Zou Z, Koop HU, Golds TJ (2002) Efficient plastid transformation in tobacco using the aphA-6 gene and kanamycin selection. Mol Genet Genomics 268:19–27
Iamtham S, Day A (2000) Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat Biotechnol 18:1172–1176
Kanamoto H, Yamashita A, Asao H, Okumura S, Takase H, Hattori M, Yokota A, Tomizawa K (2006) Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids. Transgenic Res 15:205–217
Kim J, Mullet JE (1994) Ribosome-binding sites on chloroplast rbcL and psbA mRNAs and light-induced initiation of D1 translation. Plant Mol Biol 25:437–448
Klaus SM, Huang FC, Eibl C, Koop HU, Golds TJ (2003) Rapid and proven production of transplastomic tobacco plants by restoration of pigmentation and photosynthesis. Plant J 35:811–821
Klein TM, Wolf ED, Wu R, Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70–73
Knoblauch M, Hibberd JM, Gray JC, van Bel AJ (1999) A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes. Nat Biotechnol 17:906–909
Koop HU, Steinmuller K, Wagner H, Rossler C, Eibl C, Sacher L (1996) Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation. Planta 199:193–201
Kumar S, Dhingra A, Daniell H (2004a) Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance. Plant Physiol 136:2843–2854
Kumar S, Dhingra A, Daniell H (2004b) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56:203–216
Kuroda H, Maliga P (2001a) Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilizes the plastid mRNAs. Nucleic Acids Res 29:970–975
Kuroda H, Maliga P (2001b) Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts. Plant Physiol 125:430–436
Kuroda H, Maliga P (2003) The plastid clpP1 protease gene is essential for plant development. Nature 425:86–89
Lee L-Y, Gelvin SB (2008) T-DNA binary vectors and systems. Plant Physiol 146:325–332
Lee SM, Kang K, Chung H, Yoo SH, Xu XM, Lee SB, Cheong JJ, Daniell H, Kim M (2006) Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny. Mol Cells 21:401–410
Liu CW, Lin CC, Chen JJ, Tseng MJ (2007) Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep 26:1733–1744
Lohse M, Drechsel O, Bock R (2007) OrganellarGenomeDRAW (OGDRAW): a tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes. Curr Genet 52:267–274
Lossl A, Bohmert K, Harloff H, Eibl C, Muhlbauer S, Koop H-U (2005) Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutropha phb Operon in Transplastomic Tobacco. Plant Cell Physiol. 46:1462–1471
Lutz KA, Maliga P (2007) Construction of marker-free transplastomic plants. Curr Opin Biotechnol 18:107–114
Lutz KA, Maliga P (2008) Plastid genomes in a regenerating tobacco shoot derive from a small number of copies selected through a stochastic process. Plant J 56:975–983
Lutz KA, Knapp JE, Maliga P (2001) Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol 125:1585–1590
Lutz KA, Corneille S, Azhagiri AK, Svab Z, Maliga P (2004) A novel approach to plastid transformation utilizes the phiC31 phage integrase. Plant J 37:906–913
Lutz KA, Azhagiri AK, Tungsuchat-Huang T, Maliga P (2007) A guide to choosing vectors for transformation of the plastid genome of higher plants. Plant Physiol 145:1201–1210
Magee AM, Horvath EM, Kavanagh TA (2004) Pre-screening plastid transgene expression cassettes in Escherichia coli may be unreliable as a predictor of expression levels in chloroplast-transformed plants. Plant Sci 166:1605–1611
Maliga P (2004) Plastid transformation in higher plants. Annu Rev Plant Physiol Plant Mol Biol 55:289–313
McBride KE, Schaaf DJ, Daley M, Stalker DM (1994) Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. Proc Natl Acad Sci USA 91:7301–7305
Melanie Oey MLBKRB (2009) Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic. Plant J 57:436–445
Muhlbauer SK, Koop HU (2005) External control of transgene expression in tobacco plastids using the bacterial lac repressor. Plant J 43:941–946
Muhlbauer SK, Lossl A, Tzekova L, Zou Z, Koop HU (2002) Functional analysis of plastid DNA replication origins in tobacco by targeted inactivation. Plant J 32:175–184
Nadai M, Bally J, Vitel M, Job C, Tissot G, Botterman J, Dubald M (2009) High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts. Transgenic Res 18:173–183
Nguyen TT, Nugent G, Cardi T, Dix PJ (2005) Generation of homoplasmic plastid transformants of a commercial cultivar of potato (Solanum tuberosum L.). Plant Sci 168:1495–1500
Nugent GD, Coyne S, Nguyen TT, Kavanagh TA, Dix PJ (2006) Nuclear and plastid transformation of Brassica oleracea var. botrytis (cauliflower) using PEG-mediated uptake of DNA into protoplasts. Plant Sci 170:135–142
Okumura S, Sawada M, Park YW, Hayashi T, Shimamura M, Takase H, Tomizawa K (2006) Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts. Transgenic Res 15:637–646
Quesada-Vargas T, Ruiz ON, Daniell H (2005) Characterization of heterologous multigene operons in transgenic chloroplasts: transcription, processing, and translation. Plant Physiol 138:1746–1762
Ruf S, Biehler K, Bock R (2000) A small chloroplast-encoded protein as a novel architectural component of the light-harvesting antenna. J Cell Biol 149:369–378
Ruf S, Hermann M, Berger IJ, Carrer H, Bock R (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 19:870–875
Shiina T, Allison L, Maliga P (1998) rbcL Transcript levels in tobacco plastids are independent of light: reduced dark transcription rate is compensated by increased mRNA stability. Plant Cell 10:1713–1722
Sidorov VA, Kasten D, Pang SZ, Hajdukiewicz PT, Staub JM, Nehra NS (1999) Technical Advance: Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19:209–216
Sikdar SR, Serino G, Chaudhuri S, Maliga P (1998) Plastid transformation of Arabidopsis thaliana. Plant Cell Rep 18:20–24
Skarjinskaia M, Svab Z, Maliga P (2003) Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea. Transgenic Res 12:115–122
Staub JM, Maliga P (1993) Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA. EMBO J 12:601–606
Staub JM, Maliga P (1995) Expression of a chimeric uidA gene indicates that polycistronic mRNAs are efficiently translated in tobacco plastids. Plant J 7:845–848
Suzuki JY, Maliga P (2000) Engineering of the rpl23 gene cluster to replace the plastid RNA polymerase alpha subunit with the Escherichia coli homologue. Curr Genet 38:218–225
Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci USA 90:913–917
Svab Z, Hajdukkiewicz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87:8526–8530
Thomas MR, Rose RJ (1983) Plastid number and plastid structural changes associated with tobacco mesophyll protoplast culture and plant regeneration. Planta 158:329–338
Verma D, Daniell H (2007) Chloroplast vector systems for biotechnology applications. Plant Physiol 145:1129–1143
Verma D, Samson NP, Koya V, Daniell H (2008) A protocol for expression of foreign genes in chloroplasts. Nat Protoc 3:739–758
Ye GN, Hajdukiewicz PT, Broyles D, Rodriguez D, Xu CW, Nehra N, Staub JM (2001) Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. Plant J 25:261–270
Zhou F, Karcher D, Bock R (2007) Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Plant J 52:961–972
Zou Z, Eibl C, Koop HU (2003) The stem-loop region of the tobacco psbA 5'UTR is an important determinant of mRNA stability and translation efficiency. Mol Genet Genomics 269:340–349
Zoubenko OV, Allison LA, Svab Z, Maliga P (1994) Efficient targeting of foreign genes into the tobacco plastid genome. Nucleic Acids Res 22:3819–3824
Zubko MK, Zubko EI, van Zuilen K, Meyer P, Day A (2004) Stable transformation of petunia plastids. Transgenic Res 13:523–530
Acknowledgement
The authors would like to thank Mrs. Doris Schäfer for preparing Fig. 2.2.
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Warzecha, H., Hennig, A. (2010). Plastid Transformation. In: Kempken, F., Jung, C. (eds) Genetic Modification of Plants. Biotechnology in Agriculture and Forestry, vol 64. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02391-0_2
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