• Keerti S. RathoreEmail author
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 64)


Cotton plant is the most important source of natural fiber. The plant also provides seeds, linters, and hulls that are used for food, feed, and several other diverse applications. Traditionally, the cultivation of cotton crop has relied heavily on the use of highly toxic pesticides. Because of its susceptibility to a variety of pests and pathogens and the fact that it is a source of several useful products, the cotton plant offers a large number of targets for modification and improvement through genetic engineering. Transgenic cotton, resistant to certain insects and some herbicides, has been accepted enthusiastically by the farmers in many countries, including the United States, China, and India. This chapter describes various methods and tools used to transform cotton, important traits engineered into this plant, and novel possibilities offered for improvement by the recent advances in genetic modification technologies.


Somatic Embryogenesis Cotton Fiber Shoot Apical Meristem Cotton Plant Transgenic Cotton 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Research in the author's laboratory has been supported by funds from Cotton Incorporated, Texas Cotton Biotechnology Initiative (TxCOT), Texas Higher Education Coordinating Board (Advanced Research Program), Texas Food & Fibers Commission, and Texas Agricultural Experiment Station.


  1. Bacheler J, Mott D, Bowman DT (2006) The relative efficacy of Bollgard, Bollgard II and WideStrike lines against bollworms in North Carolina in 2003 and 2005: implications for producer choices. Proc Beltwide Cotton Conf 2006:1536–1540Google Scholar
  2. Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Johnson S, Plaetinck G, Munyikwa T, Pleau M, Vaughn T, Roberts J (2007) Control of coleopteran insect pests through RNA interference. Nat Biotechnol 25:1322–1326PubMedCrossRefGoogle Scholar
  3. Bayley C, Trolinder N, Ray C, Morgan M, Quisenberry JE, Ow DW (1992) Engineering 2,4-D resistance into cotton. Theor Appl Genet 83:645–649CrossRefGoogle Scholar
  4. Brookes G, Barfoot P (2006) GM crops: the first ten years -- global socio-economic and environmental impacts. ISAAA Briefs 36. ISAAA, Ithaca, N.Y.Google Scholar
  5. Chapman KD, Neogi PB, Hake KD, Stawska AA, Speed TR, Cotter MQ, Garrett DC, Kerby T, Richardson CD, Ayre BG, Ghosh S, Kinney AJ (2008) Reduced oil accumulation in cottonseeds transformed with a Brassica non-functional allele of a delta-12 fatty acid desaturase (FAD2). Crop Sci 48:1470–1481CrossRefGoogle Scholar
  6. Chen YS, Hubmeier C, Tran M, Martens A, Cerny RE, Sammons RD, CaJacob C (2006) Expression of CP4 EPSPS in microspores and tapetum cells of cotton (Gossypium hirsutum) is critical for male reproductive development in response to late-stage glyphosate applications. Plant Biotechnol J 4:477–487PubMedGoogle Scholar
  7. Chlan CA, Rajasekaran K, Cleveland TE (2000) Transgenic cotton (Gossypium hirsutum) In: Bajaj YPS (ed) Transgenic crops I. Biotechnology in agriculture and forestry, vol 46. Springer, Heidelberg, pp 283–301Google Scholar
  8. Chowdhury B, John ME (1998) Thermal evaluation of transgenic cotton containing polyhydroxybutyrate. Thermochim Acta 313:43–53CrossRefGoogle Scholar
  9. Ellis MH, Millar AA, Llewellyn DJ, Peacock WJ, Dennis ES (2000) Transgenic cotton (Gossypium hirsutum) over-expressing alcohol dehydrogenase shows increased ethanol fermentation but no increase in tolerance to oxygen deficiency. Aust J Plant Physiol 27:1041–1050Google Scholar
  10. Emani C, Garcia JM, Lopata-Finch E, Pozo MJ, Uribe P, Kim D-J, Sunilkumar G, Cook DR, Kenerley CM, Rathore KS (2003) Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens. Plant Biotechnol J 1:321–336PubMedCrossRefGoogle Scholar
  11. Estruch JJ, Warren GW, Mullins MA, Nye GJ, Craig JA, Koziel MG (1996) Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Proc Natl Acad Sci USA 93:5389–5394PubMedCrossRefGoogle Scholar
  12. FAO (2008) FAO statistics. Accessed 7 Jan 2008
  13. Finer JJ, McMullen MD (1990) Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Rep 8:586–589CrossRefGoogle Scholar
  14. Firoozabady E, DeBoer DL, Merlo DJ, Halk EL, Amerson LN, Rashka KE, Murray EE (1987) Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Mol Biol 10:105–116CrossRefGoogle Scholar
  15. Gruere GP, Mehta-Bhatt P, Sengupta D (2008) Bt cotton and farmer suicides in India: reviewing the evidence. IFPRI discussion paper 808. International Food Policy Research Institute, Delhi.
  16. Haigler CH, Singh B, Zhang D, Hwang S, Wu C, Cai WX, Hozain M, Kang W, Kiedaisch B, Strauss RE, Hequet EF, Wyatt BG, Jividen GM, Haladay AS (2007) Transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions. Plant Mol Biol 63:815–832PubMedCrossRefGoogle Scholar
  17. He C, Yan J, Shen G, Fu L, Holaday AS, Auld D, Blumwald E, Zhang H (2005) Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field. Plant Cell Physiol 46:1848–1854PubMedCrossRefGoogle Scholar
  18. Hedin PA, Parrott WL, Jenkins JN (1992) Relationships of glands, cotton square terpenoid aldehydes, and other allelochemicals to larval growth of Heliothis virescens (Lepidoptera, Noctuidae). J Econ Entomol 85:359–364Google Scholar
  19. Huang G, Allen R, Davis EL, Baum TJ, Hussey RS (2006) Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proc Natl Acad Sci USA 103:14302–14306PubMedCrossRefGoogle Scholar
  20. James C (2007) Global status of commercialized biotech/GM crops: 2007. ISAAA Briefs 37. ISAAA, Ithaca, N.Y.Google Scholar
  21. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907PubMedGoogle Scholar
  22. Jenkins JA, McCarty JC, Buehler RE, Kiser J, Williams C, Wofford T (1997) Resistance of cotton with endotoxin genes from Bacillus thuringiensis var. kurstaki on selected lepidoptaran insects. Agron J 89:768–780CrossRefGoogle Scholar
  23. John ME (1997) Cotton crop improvement through genetic engineering. Crit Rev Biotechnol 17:185–208CrossRefGoogle Scholar
  24. John ME, Keller G (1996) Metabolic pathway engineering in cotton: biosynthesis of polyhydroxybutyrate in fiber cells. Proc Natl Acad Sci USA 93:12768–12773PubMedCrossRefGoogle Scholar
  25. Jones K, Kerby T, Collins H, Wofford T, Bates M, Presley J, Burgess J, Beuhler B, Deaton R (1996) Performance of NuCotn with Bollgard. Proc Beltwide Cotton Conf 1996:46–47Google Scholar
  26. Keller G, Spatola L, McCabe D, Martinell B, Swain W, John ME (1997) Transgenic cotton resistant to herbicide bialaphos. Transgenic Res 6:385–392CrossRefGoogle Scholar
  27. Kornyeyev D, Logan BA, Payton P, Allen RD, Holaday AS (2001) Enhanced photochemical light utilization and decreased chilling-induced photoinhibition of photosystem II in cotton overexpressing genes encoding chloroplast-targeted antioxidant enzymes. Physiol Plant 113:323-331PubMedCrossRefGoogle Scholar
  28. Kornyeyev D, Logan BA, Payton P, Allen RD, Holaday AS (2003a) Elevated chloroplastic glutathione reductase activities decrease chilling-induced photoinhibition by increasing rates of photochemistry, but not thermal energy dissipation, in transgenic cotton. Funct Plant Biol 30:101–110CrossRefGoogle Scholar
  29. Kornyeyev D, Logan BA, Allen RD, Holaday AS (2003b) Effect of chloroplastic overproduction of ascorbate peroxidase on photosynthesis and photoprotection in cotton leaves subjected to low temperature photoinhibition. Plant Sci 165:1033–1041CrossRefGoogle Scholar
  30. Kumar S, Dhingra A, Daniell H (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56:203–216PubMedCrossRefGoogle Scholar
  31. Kumria R, Leelavathi S, Bhatnagar RJ, Reddy VS (2003) Regeneration and genetic transformation of cotton: present status and future perspectives. Plant Tissue Cult 13:211–225Google Scholar
  32. Li X-B, Cai L, Cheng N-H, Liu J-W (2002) Molecular characterization of the cotton ghTUB1 gene that is preferentially expressed in fiber. Plant Physiol 130:666–674PubMedCrossRefGoogle Scholar
  33. Li X-B, Fan X-P, Wang X-L, Cai L, Yang WC (2005) The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell 17:859–875PubMedCrossRefGoogle Scholar
  34. Liu Q, Singh SP, Green AG (2002) High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiol 129:1732–1743PubMedCrossRefGoogle Scholar
  35. Lyon BR, Cousins YL, Llewellyn DJ, Dennis ES (1993) Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid. Transgenic Res 2:162–169CrossRefGoogle Scholar
  36. Mao Y-B, Cai WJ, Wang JW, Hong G-J, Tao X-Y, Wang L-J, Huang Y-P, Chen X-Y (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotechnol 25:1307–1313PubMedCrossRefGoogle Scholar
  37. McCabe DE, Martinell BJ (1993) Transformation of elite cotton cultivars via particle bombardment of meristems. Bio/Technol 11:596–598CrossRefGoogle Scholar
  38. McCabe DE, Martinell BJ, John ME (1998) Genetic transformation of cotton through particle bombardment. In: Bajaj YPS (ed) Cotton. Biotechnology in agriculture and forestry, vol 42. Springer, Heidelberg, pp 263–273Google Scholar
  39. McCaffery A, Caprio M, Jackson R, Marcus M, Martin T, Dickerson D, Negrotto D, O'Reilly D, Chen E, Lee M (2006) Effective IRM with the novel insecticidal protein Vip3A. Proc Beltwide Cotton Conf 2006:1229–1235Google Scholar
  40. McFadden H, de Feyter R, Murray F, Grover A., Llewellyn D, Dennis E, Peacock WJ (2000) Genetic engineering approaches to the improvement of cotton's tolerance to Verticillium wilt. In: Tjamos EC, Rowe RC, Heale JB, Fravel DR (eds) Advances in Verticillium research and disease management. APS, St Paul, pp 187–191Google Scholar
  41. Micinski S, Waltman WF, Spaulding HL (2006) Efficacy of WideStrike for control of the bollworm/tobacco budworm complex in northwest Lousiana. Proc Beltwide Cotton Conf 2006:1090–1094Google Scholar
  42. Murray EE, DeBoer DL, Firoozabady E (1993) Transgenic cotton. In: Kung S-D, Wu R (eds) Transgenic plants, present status and social and economic impacts, vol 2. Academic, San Diego, pp 153–168Google Scholar
  43. Murray F, Llewellyn D, McFadden H, Last D, Dennis ES, Peacock WJ (1999) Expression of the Talaromyces flavus glucose oxidase gene in cotton and tobacco reduces fungal infection, but is also phytotoxic. Mol Breed 5:219–232CrossRefGoogle Scholar
  44. Nida DL, Kolacz KH, Buehler RE, Deaton WR, Schuler WR, Armstrong TA, Taylor ML, Ebert CC, Rogan GJ, Padgette SR, Fuchs RL (1996) Glyphosate-tolerant cotton: genetic characterization and protein expression. J Agric Food Chem 44:1960–1966CrossRefGoogle Scholar
  45. Pannetier C, Giband M, Couzi P, Tan VL, Mazier M, Tourneur J, Hau B (1997) Introduction of new traits into cotton through genetic engineering: insect resistance as example. Euphytica 96:163–166CrossRefGoogle Scholar
  46. Payton P, Webb R, Kornyeyev D, Allen RD, Holaday AS (2001) Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity. J Exp Bot 52:2345–2354PubMedCrossRefGoogle Scholar
  47. Perlak FJ, Deaton RW, Armstrong TA, Fuchs RL, Sims SR, Greenplate JT, Fischhoff DA (1990) Insect resistant cotton plants. Bio/Technology 8:939–943PubMedCrossRefGoogle Scholar
  48. Perlak FJ, Oppenhuizen M, Gustafson K, Voth R, Sivasupramaniam S, Heering D, Carey B, Ihrig RA, Roberts JK (2001) Development and commercial use of Bollgard cotton in the USA – early promises versus today's reality. Plant J 27:489–501PubMedCrossRefGoogle Scholar
  49. Perkins R (2004) A new herbicide from Bayer CropScience for use in LibertyLink cotton. Proc Beltwide Cotton Conf 2004:114Google Scholar
  50. Qaim M, Zilberman D (2003) Yield effects of genetically modified crops in developing countries. Science 299:900–902PubMedCrossRefGoogle Scholar
  51. Rajasekaran K, Grula JW, Hudspeth RL, Pofelis S, Anderson DM (1996) Herbicide-resistant Acala and Coker cottons transformed with a native gene encoding mutant forms of acetohydroxyacid synthase. Mol Breed 2:307–319CrossRefGoogle Scholar
  52. Rajasekaran K, Chlan CA, Cleveland TE (2001) Tissue culture and genetic transformation of cotton. In: Jenkins JN, Saha S (eds) Genetic improvement of cotton: emerging technologies. Science Publishers, Enfield, pp 269–290Google Scholar
  53. Rajasekaran K, Cary JW, Jaynes JM, Cleveland TE (2005) Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants. Plant Biotechnol J 3:545–554PubMedCrossRefGoogle Scholar
  54. Rathore KS, Sunilkumar G, Campbell LM (2006) Cotton (Gossypium hirsutum L.). In: Wang K (ed) Agrobacterium protocols, 2nd edn, vol 1. Methods in molecular biology, Vol 343. Humana, Totowa, N.J., pp 267–279Google Scholar
  55. Rathore KS, Sunilkumar G, Cantrell RG, Hague S, Reding HK (2008) Cotton. In: Kole C, Hall TC (eds) Transgenic sugar, tuber and fiber crops. Compendium of transgenic crop plants, vol 7. Wiley--Blackwell, Chichester, pp 199–238Google Scholar
  56. Rinehart JA, Petersen MW, John ME (1996) Tissue-specific and developmental regulation of cotton gene FbL2A. Plant Physiol 112:1331–1341PubMedCrossRefGoogle Scholar
  57. Robinson E (2006) Second generation technology: what's advantage of next Bt cotton? Delta Farm, London. Google Scholar
  58. Ruan Y-L, Llewellyn DJ, Furbank RT (2003) Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell 15:952–964PubMedCrossRefGoogle Scholar
  59. Satyavathi VV, Prasad V, Gita Lakshmi B, Lakshmi Sita G (2002) High efficiency transformation protocol for three Indian cotton varieties via Agrobactrium tumefaciens. Plant Sci 162:215–223CrossRefGoogle Scholar
  60. Sunilkumar G, Rathore KS (2001) Transgenic cotton: factors influencing AgrobacteriumAgrobacterium-mediated transformation and regeneration. Mol Breed 8:37–52CrossRefGoogle Scholar
  61. Sunilkumar G, Connell JP, Smith CW, Reddy AS, Rathore KS (2002a) Cotton α-globulin promoter: isolation and functional characterization in transgenic cotton, Arabidopsis, and tobacco. Transgenic Res 11:347–359PubMedCrossRefGoogle Scholar
  62. Sunilkumar G, Mohr L, Lopata-Finch E, Emani C, Rathore KS (2002b) Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Mol Biol 50:463–474PubMedCrossRefGoogle Scholar
  63. Sunilkumar G, Campbell LM, Hossen M, Connell JP, Hernandez E, Reddy AS, Smith CW, Rathore KS (2005) A comprehensive study of the use of a homologous promoter in antisense cotton lines exhibiting a high seed oleic acid phenotype. Plant Biotechnol J 3:319–330PubMedCrossRefGoogle Scholar
  64. Sunilkumar G, Campbell LM, Puckhaber L, Stipanovic RD, Rathore KS (2006) Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc Natl Acad Sci USA 103:18054–18059PubMedCrossRefGoogle Scholar
  65. Townsend BJ, Poole A, Blake CJ, Llewellyn DJ (2005) Antisense suppression of a (+)-δ-cadinene synthase gene in cotton prevents the induction of this defense response gene during bacterial blight infection but not its constitutive expression. Plant Physiol 138:516–528PubMedCrossRefGoogle Scholar
  66. Trolinder NL, Xhixian C (1989) Genotype specificity of the somatic embryogenesis response in cotton. Plant Cell Rep 8:133–136CrossRefGoogle Scholar
  67. Umbeck P, Johnson G, Barton K, Swain W (1987) Genetically transformed cotton (Gossypium hirsutum L.) plants. Bio/Technology 5:263–266CrossRefGoogle Scholar
  68. Wang S, Wang J-W, Yu N, Li C-H, Luo B, Gou J-Y, Wang L-J, Chen X-Y (2004a) Control of plant trichome development by a cotton fiber MYB gene. Plant Cell 16:2323–2334PubMedCrossRefGoogle Scholar
  69. Wang YQ, Chen DJ, Wang DM, Huang QS, Yao ZP, Liu FJ, Wei XW, Li RJ, Zhang ZN, Sun YR (2004b) Over-expression of Gastrodia anti-fungal protein enhances Verticillium wilt resistance in coloured cotton. Plant Breed 123:454–459CrossRefGoogle Scholar
  70. Wilkins TA, Rajasekaran K, Anderson DM (2000) Cotton biotechnology. CRC Cr Rev Plant Sci 19:511–550CrossRefGoogle Scholar
  71. Wu J, Luo X, Guo H, Xiao J, Tian Y (2006) Transgenic cotton expressing Amaranthus caudatus agglutinin, confers enhanced resistance to aphids. Plant Breed 125:390–394CrossRefGoogle Scholar
  72. Yadav BC, Veluthambi K, Subramaniam K (2006) Host-generated double stranded RNA induces RNAi in plant-parasitic nematodes and protects the host from infection. Mol Biochem Parasit 148:219–222CrossRefGoogle Scholar
  73. Yan J, He C, Wang J, Mao Z, Holaday SA, Allen RD, Zhang H (2004) Overexpression of the Arabidopsis 14-3-3 protein GF14l in cotton leads to a “Stay-Green” phenotype and improves stress tolerance under moderate drought conditions. Plant Cell Physiol 45:1007–1014PubMedCrossRefGoogle Scholar
  74. Yuceer SU, Koc NK (2006) Agrobacterium-mediated transformation and regeneration of cotton plants. Russ J Plant Physiol 53:413–417CrossRefGoogle Scholar
  75. Zapata C, Park SH, El-Zik KM, Smith RH (1999) Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl Genet 98:252–256CrossRefGoogle Scholar
  76. Zhang D, Hrmova M, Wan C-H, Wu C, Balzen J, Cai W, Wang J, Densmore LD, Fincher GB, Zhang H, Haigler CH (2004) Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls. Plant Mol Biol 54:353–372PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Institute for Plant Genomics & Biotechnology and Department of Soil and Crop ScienceTexas A&M UniversityCollege StationUSA

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