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Metabolic Engineering

  • Lars M. VollEmail author
  • Frederik Börnke
Chapter
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 64)

Abstract

The metabolic engineering of primary metabolism provides an enormous potential to improve the value of plant-based raw materials for food and industrial applications. This chapter focuses on the current progress in the manipulation of carbohydrate and lipid biosynthesis in transgenic starch and oilseed crops, respectively. In most approaches, the manipulation of the biosynthetic routes revealed limitations and bottlenecks in the pathways, and we discuss how the gain of knowledge led to improved strategies to circumvent these restrictions. We summarize recent advances in improving starch yield as well as starch quality, i.e. the ratio of amylopectin to amylose, for particular industrial uses, and we present the strategies to produce novel beneficial carbohydrates in crops. We also describe the numerous approaches to obtain high value plant oil by the targeting of unusual fatty acids into the seed oil of oilseed crops by introducing biosynthetic enzymes of exotic species in order to improve the suitability of the engineered vegetable oil as renewable resource for biofuel production, as chemical feedstock and for food and feed. Finally, we briefly discuss recent successful examples to engineer plant secondary metabolism to enhance plant nutritional value

Keywords

Potato Tuber Metabolic Engineering Erucic Acid Acyl Carrier Protein Starch Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Agius F, Gonzalez-Lamothe R, Caballero JL, Munoz-Blanco J, Botella MA, Valpuesta V (2003) Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase. Nat Biotechnol 21:177–181PubMedCrossRefGoogle Scholar
  2. Al-Babili S, Beyer P (2005) Golden Rice -- five years on the road -- five years to go? Trends Plant Sci 10:565–573PubMedCrossRefGoogle Scholar
  3. Altmann T, Felix G, Jessop A, Kauschmann A, Uwer U, Penacortes H, Willmitzer L (1995) Ac/Ds transposon mutagenesis in Arabidopsis thaliana -- mutant spectrum and frequency of Ds insertion mutants. Mol Gen Genet 247:646–652PubMedCrossRefGoogle Scholar
  4. An S, Park S, Jeong D-H, Lee D-Y, Kang H-G, Yu J-H, Hur J, Kim S-R, Kim Y-H, Lee M, Han S, Kim S-J, Yang J, Kim E, Wi SJ, Chung HS, Hong J-P, Choe V, Lee H-K, Choi J-H, Nam J, Kim S-R, Park P-B, Park KY, Kim WT, Choe S, Lee C-B, An G (2003) Generation and analysis of end sequence database for T-DNA tagging lines in rice. Plant Physiol 133:2040–2047PubMedCrossRefGoogle Scholar
  5. ap Rees T, Hill SA (1994) Metabolic control analysis of plant metabolism. Plant Cell Environ 17:587–599CrossRefGoogle Scholar
  6. Bailey JE (1991) Toward a science of metabolic engineering. Science 252:1668–1675PubMedCrossRefGoogle Scholar
  7. Bates PD, Ohlrogge JB, Pollard M (2007) Incorporation of newly synthesized fatty acids into cytosolic glycerolipids in pea leaves occurs via acyl editing. J Biol Chem 282:31206–31216PubMedCrossRefGoogle Scholar
  8. Beyer P, Al-Babili S, Ye XD, Lucca P, Schaub P, Welsch R, Potrykus I (2002) Golden rice: Introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency. J Nutr 132:506S–510SPubMedGoogle Scholar
  9. Börnke F, Hajirezaei M, Sonnewald U (2001) Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici. J Bacteriol 183:2425–2430PubMedCrossRefGoogle Scholar
  10. Börnke F, Hajirezaei M, Heineke D, Melzer M, Herbers K, Sonnewald U (2002) High-level production of the non-cariogenic sucrose isomer palatinose in transgenic tobacco plants strongly impairs development. Planta 214:356–364PubMedCrossRefGoogle Scholar
  11. Broun P (2004) Transcription factors as tools for metabolic engineering in plants. Curr Opin Plant Biol 7:202–209PubMedCrossRefGoogle Scholar
  12. Broun P, Somerville C (1997) Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean. Plant Physiol 113:933–942PubMedCrossRefGoogle Scholar
  13. Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S, Schijlen EG, Hall RD, Bovy AG, Luo J, Martin C (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26:1301–1308PubMedCrossRefGoogle Scholar
  14. Cahoon EB, Ohlrogge JB (1994) Apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing Umbelliferae endosperm. Plant Physiol 104:845–855PubMedGoogle Scholar
  15. Cahoon EB, Carlson TJ, Ripp KG, Schweiger BJ, Cook GA, Hall SE, Kinney AJ (1999) Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos. Proc Natl Acad Sci USA 96:12935–12940PubMedCrossRefGoogle Scholar
  16. Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hitz WD, Coughlan SJ (2003) Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nat Biotechnol 21:1082–1087PubMedCrossRefGoogle Scholar
  17. Cahoon EB, Dietrich CR, Meyer K, Damude HG, Dyer JM, Kinney AJ (2006) Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds. Phytochem 67:1166–1176CrossRefGoogle Scholar
  18. Cahoon EB, Shockey JM, Dietrich CR, Gidda SK, Mullen RT, Dyer JM (2007) Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Curr Opin Plant Biol 10:236–244PubMedCrossRefGoogle Scholar
  19. Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne S (2000) Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97:6487–6492PubMedCrossRefGoogle Scholar
  20. Davuluri GR, van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Brummell DA, King SR, Palys J, Uhlig J, Bramley PM, Pennings HMJ, Bowler C (2005) Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol 23:890–895PubMedCrossRefGoogle Scholar
  21. Dehesh K (2001) How can we genetically engineer oilseed crops to produce high levels of medium-chain fatty acids? Eur J Lipid Sci Technol103:688–697CrossRefGoogle Scholar
  22. DellaPenna D, Last RL (2006) Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol Plant 126:356–368CrossRefGoogle Scholar
  23. DellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu Rev Plant Biol 57:711–738PubMedCrossRefGoogle Scholar
  24. Durrett TP, Benning C, Ohlrogge J (2008) Plant triacylglycerols as feedstocks for the production of biofuels. Plant J 54:593–607PubMedCrossRefGoogle Scholar
  25. Dyer JM, Chapital DC, Kuan JW, Mullen RT, Pepperman AB (2002) Metabolic engineering of Saccharomyces cerevisiae for production of novel lipid compounds. Appl Microbiol Biotechnol 59:224–230PubMedCrossRefGoogle Scholar
  26. Dyer JM, Stymne S, Green AG, Carlsson AS (2008) High-value oils from plants. Plant J 54: 640–655PubMedCrossRefGoogle Scholar
  27. Fernie AR, Willmitzer L, Trethewey RN (2002) Sucrose to starch: a transition in molecular plant physiology. Trends Plant Sci 7:35–41PubMedCrossRefGoogle Scholar
  28. Geigenberger P (2003) Regulation of sucrose to starch conversion in growing potato tubers. J Exp Bot 54:457–465PubMedCrossRefGoogle Scholar
  29. Geigenberger P, Stamme C, Tjaden J, Schulz A, Quick PW, Betsche T, Kersting HJ, Neuhaus HE (2001) Tuber physiology and properties of starch from tubers of transgenic potato plants with altered plastidic adenylate transporter activity. Plant Physiol 125:1667–1678PubMedCrossRefGoogle Scholar
  30. Geigenberger P, Stitt M, Fernie AR (2004) Metabolic control analysis and regulation of the conversion of sucrose to starch in growing potato tubers. Plant Cell Environ 27:655–673CrossRefGoogle Scholar
  31. Grotewold E (2008) Transcription factors for predictive plant metabolic engineering: are we there yet? Curr Opin Biotechnol 19:138–144PubMedCrossRefGoogle Scholar
  32. Hellwege EM, Czapla S, Jahnke A, Willmitzer L, Heyer AG (2000) Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. Proc Natl Acad Sci USA 97:8699–8704PubMedCrossRefGoogle Scholar
  33. Henikoff S, Till BJ, Comai L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol 135:630–636PubMedCrossRefGoogle Scholar
  34. Herbers K (2003) Vitamin production in transgenic plants. J Plant Physiol 160:821–829PubMedCrossRefGoogle Scholar
  35. Hoffmann M, Wagner M, Abbadi A, Fulda M, Feussner I (2008) Metabolic engineering of omega3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J Biol Chem 283:22352–22362PubMedCrossRefGoogle Scholar
  36. Jacobsen JR, Khosla C (1998) New directions in metabolic engineering. Curr Opin Chem Biol 2:133–137PubMedCrossRefGoogle Scholar
  37. Jako C, Kumar A, Wei Y, Zou J, Barton DL, Giblin EM, Covello PS, Taylor DC (2001) Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol 126:861–874PubMedCrossRefGoogle Scholar
  38. Jeon J-S, Lee S, Jung K-H, Jun S-H, Jeong D-H, Lee J, Kim C, Jang S, Lee S, Yang K, Nam J, An K, Han M-J, Sung R-J, Choi H-S, Yu J-H, Choi J-H, Cho S-Y, Cha S-S, Kim S-I, An G (2000) T-DNA insertional mutagenesis for functional genomics in rice. Plant J 22:561–570PubMedCrossRefGoogle Scholar
  39. Jobling S (2004) Improving starch for food and industrial applications. Curr Opin Plant Biol 7:210–218PubMedCrossRefGoogle Scholar
  40. Jobling SA, Jarman C, Teh MM, Holmberg N, Blake C, Verhoeyen ME (2003) Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat Biotechnol 21:77–80PubMedCrossRefGoogle Scholar
  41. Kammerer B, Fischer K, Hilpert B, Schubert S, Gutensohn M, Weber A, Flügge UI (1998) Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: The glucose 6-phosphate phosphate antiporter. Plant Cell 10:105–117PubMedGoogle Scholar
  42. Kim CM, Piao HL, Park SJ, Chon NS, Je BI, Sun B, Park SH, Park JY, Lee EJ, Kim MJ, Chung WS, Lee KH, Lee YS, Lee JJ, Won YJ, Yi G, Nam MH, Cha YS, Yun DW, Eun MY, Han C-d (2004) Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice. Plant J 39:252–263PubMedCrossRefGoogle Scholar
  43. Kinney AJ (2006) Metabolic engineering in plants for human health and nutrition. Curr Opin Biotechnol 17:130–138PubMedCrossRefGoogle Scholar
  44. Knutzon DS, Hayes TR, Wyrick A, Xiong H, Maelor Davies H, Voelker TA (1999) Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiol 120:739–746PubMedCrossRefGoogle Scholar
  45. Kroon JTM, Wei WX, Simon WJ, Slabas AR (2006) Identification and functional expression of a type 2 acyl-CoA : diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals. Phytochemistry 67:2541–2549PubMedCrossRefGoogle Scholar
  46. Larson TR, Edgell T, Byrne J, Dehesh K, Graham IA (2002) Acyl CoA profiles of transgenic plants that accumulate medium-chain fatty acids indicate inefficient storage lipid synthesis in developing oilseeds. Plant J 32:519–527PubMedCrossRefGoogle Scholar
  47. Lee M, Lenman M, Banas A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, Sjodahl S, Green A, Stymne S (1998) Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation. Science 280:915–918PubMedCrossRefGoogle Scholar
  48. Mansoor S, Amin I, Hussain M, Zafar Y, Briddon RW (2006) Engineering novel traits in plants through RNA interference. Trends Plant Sci 11:559–565PubMedCrossRefGoogle Scholar
  49. Metzger JO, Bornscheuer U (2006) Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification. Appl Microbiol Biotechnol 71:13–22PubMedCrossRefGoogle Scholar
  50. Meyer FD, Talbert LE, Martin JM, Lanning SP, Greene TW, Giroux MJ (2007) Field evaluation of transgenic wheat expressing a modified ADP-glucose pyrophosphorylase large subunit. Crop Sci 47:336–342CrossRefGoogle Scholar
  51. Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Ric De Vos CH, van Tunen AJ, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19:470–474PubMedCrossRefGoogle Scholar
  52. Nath U, Becker HC, Möllers C (2006) Increasing erucic acid content in rapeseed. In: OIRA (ed) Oils, fats and lipids for a healthier future, Madrid. Proc Eur Fed Lipid Congr 4:OIRA-003Google Scholar
  53. Ohlrogge J, Browse J (1995) Lipid biosynthesis. Plant Cell 7:957–970PubMedGoogle Scholar
  54. Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, Vernon G, Wright SY, Hinchliffe E, Adams JL, Silverstone AL, Drake R (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nat Biotechnol 23:482–487PubMedCrossRefGoogle Scholar
  55. Qi B, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier JA, Stobart AK, Lazarus CM (2004) Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol 22:739–745PubMedCrossRefGoogle Scholar
  56. Regierer B, Fernie AR, Springer F, Perez-Melis A, Leisse A, Koehl K, Willmitzer L, Geigenberger P, Kossmann J (2002) Starch content and yield increase as a result of altering adenylate pools in transgenic plants. Nat Biotechnol 20:1256–1260PubMedCrossRefGoogle Scholar
  57. Ritsema T, Smeekens SCM (2003) Engineering fructan metabolism in plants. J Plant Physiol 160:811–820PubMedCrossRefGoogle Scholar
  58. Sayanova O, Haslam R, Venegas Caleron M, Napier JA (2007) Cloning and characterization of unusual fatty acid desaturases from Anemone leveillei: identification of an acyl-coenzyme A C20 Delta5-desaturase responsible for the synthesis of sciadonic acid. Plant Physiol 144:455–467PubMedCrossRefGoogle Scholar
  59. Schijlen EGWM, Ric de Vos CH, van Tunen AJ, Bovy AG (2004) Modification of flavonoid biosynthesis in crop plants. Phytochemistry 65:2631–2648PubMedCrossRefGoogle Scholar
  60. Schultz DJ, Suh MC, Ohlrogge JB (2000) Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin. Plant Physiol 124:681–692PubMedCrossRefGoogle Scholar
  61. Sevenier R, Hall RD, van der Meer IM, Hakkert HJ, van Tunen AJ, Koops AJ (1998) High level fructan accumulation in a transgenic sugar beet. Nat Biotechnol 16:843–846PubMedCrossRefGoogle Scholar
  62. Shintani D, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282:2098–2100PubMedCrossRefGoogle Scholar
  63. Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM (2006) Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18:2294–2313PubMedCrossRefGoogle Scholar
  64. Smidansky ED, Clancy M, Meyer FD, Lanning SP, Blake NK, Talbert LE, Giroux MJ (2002) Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield. Proc Natl Acad Sci USA 99:1724–1729PubMedCrossRefGoogle Scholar
  65. Smidansky ED, Martin JM, Hannah LC, Fischer AM, Giroux MJ (2003) Seed yield and plant biomass increases in rice are conferred by deregulation of endosperm ADP-glucose pyrophosphorylase. Planta 216:656–664PubMedGoogle Scholar
  66. Smidansky ED, Meyer FD, Blakeslee B, Weglarz TE, Greene TW, Giroux MJ (2007) Expression of a modified ADP-glucose pyrophosphorylase large subunit in wheat seeds stimulates photosynthesis and carbon metabolism. Planta 225:965–976PubMedCrossRefGoogle Scholar
  67. Stark DM, Timmerman KP, Barry GF, Preiss J, Kishore GM (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258:287–292PubMedCrossRefGoogle Scholar
  68. Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, Lambert W, Van der Straeten D (2007) Folate fortification of rice by metabolic engineering. Nat Biotechnol 25:1277–1279PubMedCrossRefGoogle Scholar
  69. Suh MC, Schultz DJ, Ohlrogge JB (2002) What limits production of unusual monoenoic fatty acids in transgenic plants? Planta 215:584–595PubMedCrossRefGoogle Scholar
  70. Sweetlove LJ, Burrell MM, ap Rees T (1996) Characterization of transgenic potato (Solanum tuberosum) tubers with increased ADP glucose pyrophosphorylase. Biochem J 320:487–492PubMedGoogle Scholar
  71. Tang GL, Galili G, Zhuang X (2007) RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering. Metabolomics 3: 357–369CrossRefGoogle Scholar
  72. Thomaeus S, Carlsson AS, Stymne S (2001) Distribution of fatty acids in polar and neutral lipids during seed development in Arabidopsis thaliana genetically engineered to produce acetylenic, epoxy and hydroxy fatty acids. Plant Sci 161:997–1003CrossRefGoogle Scholar
  73. Tjaden J, Möhlmann T, Kampfenkel K, Henrichs G, Neuhaus HE (1998) Altered plastidic ATP/ADP-transporter activity influences potato (Solanum tuberosum L.) tuber morphology, yield and composition of tuber starch. Plant J 16:531–540CrossRefGoogle Scholar
  74. Tomlinson K, Denyer K (2003) Starch synthesis in cereal grains. Adv Bot Res 40:1–61CrossRefGoogle Scholar
  75. van de Loo FJ, Broun P, Turner S, Somerville C (1995) An oleate 12-hydroxylase from Ricinus communis l is a fatty acyl desaturase homolog. Proc Natl Acad Sci USA 92:6743–6747PubMedCrossRefGoogle Scholar
  76. Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL (2003) Engineering vitamin E content: from Arabidopsis mutant to soy oil. Plant Cell 15:3007–3019PubMedCrossRefGoogle Scholar
  77. Visser RGF, Somhorst I, Kuipers GJ, Ruys NJ, Feenstra WJ, Jacobsen E (1991) Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol Gen Genet 225:289–296PubMedCrossRefGoogle Scholar
  78. Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257:72–74PubMedCrossRefGoogle Scholar
  79. Weyens G, Ritsema T, Van Dun K, Meyer D, Lommel M, Lathouwers J, Rosquin I, Denys P, Tossens A, Nijs M, Turk S, Gerrits N, Bink S, Walraven B, Lefebvre M, Smeekens S (2004) Production of tailor-made fructans in sugar beet by expression of onion fructosyltransferase genes. Plant Biotechnol J 2:321–327PubMedCrossRefGoogle Scholar
  80. Wiberg E, Edwards P, Byrne J, Stymne S, Dehesh K (2000) The distribution of caprylate, caprate and laurate in lipids from developing and mature seeds of transgenic Brassica napus L. Planta 212:33–40PubMedCrossRefGoogle Scholar
  81. Wu GH, Truksa M, Datla N, Vrinten P, Bauer J, Zank T, Cirpus P, Heinz E, Qiu X (2005) Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat Biotechnol 23:1013–1017PubMedCrossRefGoogle Scholar
  82. Wu L, Birch RG (2007) Doubled sugar content in sugarcane plants modified to produce a sucrose isomer. Plant Biotechnol J 5:109–117PubMedCrossRefGoogle Scholar
  83. Ye X, Al-Babili S, Ouml KL, Ti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305PubMedCrossRefGoogle Scholar
  84. Yu K, McCracken CT, Jr, Li R, Hildebrand DF (2006) Diacylglycerol acyltransferases from Vernonia and Stokesia prefer substrates with vernolic acid. Lipids 41:557–566PubMedCrossRefGoogle Scholar
  85. Zhang L, Häusler RE, Greiten C, Hajirezaei MR, Haferkamp I, Neuhaus HE, Flügge UI, Ludewig F (2008) Overriding the co-limiting import of carbon and energy into tuber amyloplasts increases the starch content and yield of transgenic potato plants. Plant Biotechnol J 6:453–464PubMedCrossRefGoogle Scholar
  86. Zhu C, Naqvi S, Gomez-Galera S, Pelacho AM, Capell T, Christou P (2007) Transgenic strategies for the nutritional enhancement of plants. Trends Plant Sci 12:548–555PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Lehrstuhl für BiochemieFriedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany

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