Plant Nuclear Transformation
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Many of the advances in plant biotechnology have been based on transformation; the ability to introduce DNAs into plant cells to recover whole transgenic plants. For DNA introduction to be successful, it must first pass through the cell wall and then into the nucleus, before it is integrated into host DNA. Although many different methods for DNA introduction exist, particle bombardment and Agrobacterium remain the two most commonly used approaches. Ultimately, the final outcome of plant transformation is the recovery of new phenotypes, which could not be easily recovered using conventional breeding approaches.
KeywordsTransgenic Plant Green Fluorescent Protein Pollen Tube Transient Expression Particle Bombardment
Salaries and research support were provided by the United Soybean Board and by State and Federal funds appropriated to The Ohio State University/Ohio Agricultural Research and Development Center (OSU/OARDC). Mention of proprietary products does not constitute a guarantee or warranty of the product by OSU/OARDC and also does not imply approval to the exclusion of other products that may also be suitable.
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