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A Journey with Plant Cell Division: Reflection at My Halfway Stop

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Progress in Botany 71

Part of the book series: Progress in Botany ((BOTANY,volume 71))

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Abstract

I have studied various aspects of plant cell division for more than 40 years, and here I try to illustrate some foci among them. First, establishment of the induction of cell division in freshly isolated protoplasts from leaves is described, as this is important not only for biotechnology, but also for the cell cycle transition from G0 to S phase, whose importance has not been fully understood yet. The role of plant hormones, auxin and cytokinin, on this process has not been fully elucidated. Secondly, establishment of a model plant cell system of tobacco BY-2 cells and the high level of cell cycle synchrony achieved using this system is also an important theme for me. However, I will not go into details, but instead refer to the two recently published monographs edited by myself (Nagata T, Hasezawa S, Inzé D, Biotechnology in agriculture and forestry, vol. 53, Tobacco BY-2 Cells. Springer, Berlin, 2004; Nagata T, Matsuoka K, Inzé D, Biotechnology in agriculture and forestry, vol. 58. Tobacco BY-2 cells. From cellular dynamics to omics, Springer, Berlin, 2006). Further, habituation, discovered by Gautheret (Bull Soc Chim Biol 24:13–47, 1942), will be described, and I will describe how recently we have identified key molecules that may play an important role in this process. Finally, I will try to illustrate aspects of heterophylly from our studies on Ludwigia arcuata (Onagraceae). Although heterophylly is thought to be mostly related to cell elongation in leaves and induced by environmental cues, we found that in L. arcuata, it is closely associated with cell division. This system offers an opportunity to understand the role of cell division upon leaf shape determination as affected by environmental cues. Thus, as my scientific issues are always associated with cell division, their aspects and background stories will be described in this chapter.

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References

  • Asada T, Sonobe S, Shibaoka H (1991) Microtubule translocation in the cytokinetic apparatus of cultured tobacco cells. Nature 350:238–241

    Article  CAS  Google Scholar 

  • Dharmasiri N, Dharmasiri S, Estelle M (2005) The F-box protein TIR1 is an auxin receptor. Nature 435:441–445

    Article  PubMed  CAS  Google Scholar 

  • Fukunaga Y, Nagata T, Takebe I (1981) Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology 113:752–760

    Article  PubMed  CAS  Google Scholar 

  • Gautheret R (1939) Sur la possibilité de réaliser la culture indéfinite des tissue de tubercules de carotte. C R Acad Sci (Paris) 208:118–120

    Google Scholar 

  • Gautheret R (1942) Hétéro-auxin et cultures de tissues végétaux. Bull Soc Chim Biol 24:13–47

    CAS  Google Scholar 

  • Haberlandt G (1902) Kulturversuche mit isolierten Pflanzenzellen. Sitzber. Akad. Wiss. Wien, Math.-Naturwiss. Kl. III:69–92

    Google Scholar 

  • Ishida S, Takahashi Y, Nagata T (1993) Isolation of cDNA of an auxin-regulated gene encoding a G protein β subunit-like protein from tobacco BY-2 cells. Proc Natl Acad Sci USA 90:11152–11156

    Article  PubMed  CAS  Google Scholar 

  • Ishii S (1976) Enzymatic maceration of plant tissues by endo-pectin lyase and endo-polygalacturonase from Aspergillus japonicus. Phytopathology 66:281–289

    Article  CAS  Google Scholar 

  • Israel HW, Steward FC (1966) The fine structure of quiescent and growing carrot cells: its relation to growth induction. Ann Bot 30:63–79

    Google Scholar 

  • Iwahara M, Saito T, Ishida S, Takahashi Y, Nagata T (1998) Isolation and characterization of a cytokinin up-regulated gene from tobacco mesophyll protoplasts. Plant Cell Physiol 39:859–864

    PubMed  CAS  Google Scholar 

  • Joshi PC, Ball E (1968) Growth of isolated palisade cells of Arachis hypogaea in vitro. Dev Biol 17:308–325

    Article  PubMed  CAS  Google Scholar 

  • Joshi PC, Noggle GR (1967) Growth of isolated mesophyll cells of Arachis hypogaea in simple defined medium in vitro. Science 158:1575–1577

    Article  PubMed  CAS  Google Scholar 

  • Kao KN, Michayluk CR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115:355–367

    Article  CAS  Google Scholar 

  • Kikkawa H, Nagata T, Takebe I (1982) Infection of protoplasts from tobacco suspension cultures by tobacco mosaic virus. J Gen Virol 63:451–456

    Article  CAS  Google Scholar 

  • Kohlenbach HW (1960) Die Streckungs- und Teilungswachstum isolierter Mesophyllzellen. Ber Deutsch Bot Ges 73:57–58

    Google Scholar 

  • Kuroiwa T, Kawano S, Nishibayashi S, Sato C (1984) Epifluorescent microscopic evidence for maternal inheritance of chloroplast DNA. Nature 298:481–483

    Article  Google Scholar 

  • Kuwabara A, Ikegami K, Koshiba T, Nagata T (2003) Effects of ethylene and abscisic acid upon heterophylly in Ludwigia arcuata (Onagraceae). Planta 217:880–887

    Article  PubMed  CAS  Google Scholar 

  • Kuwabara A, Nagata T (2006) Cellular basis of developmental plasticity observed in hetrophyllous leaf formation of Ludwigia arcuata (Ongagraceae). Planta 224:761–770

    Article  PubMed  CAS  Google Scholar 

  • Kuwabara A, Tsukaya H, Nagata T (2001) Identification of factors that cause heterophylly in Ludwigia arcuata (Onagraceae). Plant Biol 3:98–105

    Article  Google Scholar 

  • Marrs KA (1996) The functions and regulation of glutathione S-transferases in plants. Annu Rev Plant Physiol Plant Mol Biol 47:127–158

    Article  PubMed  CAS  Google Scholar 

  • Márton L, Wullems GJ, Molendijk L, Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277:129–131

    Article  Google Scholar 

  • Matsubayashi Y, Sakagami Y (1996) Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc Natl Acad Sci USA 93:7623–7627

    Article  PubMed  CAS  Google Scholar 

  • Meyer Y, Aspart L, Chartier Y (1984) Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplasts cultivated in vitro. 2. Time course and label incorporations. Plant Physiol 75:1034–1039

    Article  PubMed  CAS  Google Scholar 

  • Miyamura S, Nagata T, Kuroiwa T (1986) Quantitative fluorescence microscopy on dynamic changes of plastid nucleoids during wheat development. Protoplasma 133:66–72

    Article  CAS  Google Scholar 

  • Miyamura S, Kuroiwa T, Nagata T (1987) Disappearance of plastid and mitochondrial nucleoids during the formation of generative cells of higher plants revealed by fluorescence microscopy. Protoplasma 141:149–159

    Article  Google Scholar 

  • Nagata T (1978) A novel cell-fusion method of protoplasts by polyvinylalcohol. Naturwissenschaften 65:263–264

    Article  Google Scholar 

  • Nagata T (1984a) Fusion of somatic cells. In: Linskens HF, Heslop-Harrison J (eds) Encyclopedia of plant physiology. New series, vol. 17. Springer, Berlin, pp 491–507

    Google Scholar 

  • Nagata T (1984b) Interaction of liposomes and protoplasts as a model system of protoplast fusion. In: Beers RF Jr, Bassett EG (eds) Cell fusion: gene transfer and transformation. Raven, New York, pp 217–226

    Google Scholar 

  • Nagata T, Ishii S (1979) A rapid method for isolation of mesophyll protoplasts. Can J Bot 57:1820–1823

    Article  Google Scholar 

  • Nagata T, Kumagai F (1999) Plant cell biology through the windows of highly synchronized tobacco BY-2 cell line. Methods Cell Sci 23:123–127

    Article  Google Scholar 

  • Nagata T, Melchers G (1978) Surface charge of protoplasts and their significance in cell–cell interaction. Planta 142:235–238

    Article  CAS  Google Scholar 

  • Nagata T, Takebe I (1970) Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta 92:301–308

    Article  Google Scholar 

  • Nagata T, Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12–20

    Article  Google Scholar 

  • Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cells as the “HeLa” cell in the cell biology of higher plants. Int Rev Cytol 132:1–30

    Article  CAS  Google Scholar 

  • Nagata T, Hasezawa SA, Inzé D (2004) Biotechnology in Agriculture and Forestry, vol 53, Tobacco BY-2 Cells. Springer, Berlin

    Google Scholar 

  • Nagata T, Matsuoka KA, Inzé D (2006) Biotechnology in Agriculture and Forestry, vol 58, Tobacco BY-2 cells: from cellular dynamics to omics. Springer, Berlin

    Google Scholar 

  • Nagata T, Okada K, Takebe I, Matsui C (1981) Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet 184:161–165

    CAS  Google Scholar 

  • Sato M, Tsutsumi M, Ohtsubo E, Nishii K, Kuwabara A, Nagata T (2008) Temperature-dependent changes of cell shape during heterophyllous leaf formation in Ludwigia arcuata (Onagraceae). Planta 228:27–36

    Article  PubMed  CAS  Google Scholar 

  • Steward FC, Blakely LM, Kent AE, Mapes MO (1963) Growth and organization in free cell cultures. Brookhaven Symp Biol 16:73–88

    Google Scholar 

  • Shimizu T, Eguchi K, Nishida I, Laukens K, Witters E, Van Onckelen H, Nagata T (2006) A novel cell division factor from tobacco 2B-13 cells that induced cell division in auxin-starved tobacco BY-2 cells. Naturwissenschaften 93, 278–285

    Google Scholar 

  • Strasburger E (1892) Über das Verhalten des Pollens in der Befruchtungsvorgänge bei der Gymospermen, Histologische Beiträge. Gustav Fischer, Jena

    Google Scholar 

  • Takahashi Y, Kuroda H, Tanaka T, Machida Y, Takebe I, Nagata T (1989) Isolation of an auxin-regulated gene cDNA expressed during the transition from G0 to S phase in tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86:9297–9293

    Article  Google Scholar 

  • Takahashi Y, Nagata T (1992) parB: An auxin-regulated gene encoding glutathione S-transferase. Proc Natl Acad Sci USA 89:56–59

    Article  PubMed  CAS  Google Scholar 

  • Takahashi Y, Sakai T, Ishida S, Nagata T (1995) Identification of auxin-responsive elements of parB and their expression in apices of shoot and root. Proc Natl Acad Sci USA 90:6359–6363

    Article  Google Scholar 

  • Takebe I, Otsuki Y, Aoki S (1968) Isolation of tobacco mesophyll protoplasts in intact and active state. Plant Cell Physiol 9:115–124

    Google Scholar 

  • Takebe I, Otsuki Y (1969) Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc Natl Acad Sci USA 64:843–848

    Article  PubMed  CAS  Google Scholar 

  • Turck F, Fornara F, Coupland G (2008) Regulation and identity of florigen: Flowering locus T moves center stage. Annu Rev Plant Biol 59:573–594

    Article  PubMed  CAS  Google Scholar 

  • Van Larebeke N, Engler G, Holsters M, Van den Elsacker S, Zaenen I, Schilperoort RA, Schell J (1974) Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature 252:169–170

    Article  PubMed  Google Scholar 

  • Von Wettstein D (2006) Fascination with chloroplasts and chromosome pairing. Prog Bot 67:1–30

    Google Scholar 

  • White PR (1939) Potentially unlimited growth of excised plant callus in an artificial nutrient. Am J Bot 26:59–64

    Article  Google Scholar 

  • White PR, White PR (1964) The cultivation of animal and plant cells, 2nd edn. Ronald, New York

    Google Scholar 

  • Zelcer A, Galun E (1976) Culture of newly isolated tobacco protoplasts – Precursor incorporation into protein, RNA and DNA. Plant Sci Lett 7:331–336

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Bioscience and Applied Chemistry, Hosei University, 3-7-2 Kajino-cho, Koganei-shi, Tokyo, 185-8584, Japan

    Toshiyuki Nagata (Professor Emeritus)

  2. The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Toshiyuki Nagata (Professor Emeritus)

Authors
  1. Toshiyuki Nagata
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Editor information

Editors and Affiliations

  1. Inst. Botanik, TU Darmstadt, Schnittspahnstr. 3-5, Darmstadt, 64287, Germany

    Ulrich Lüttge

  2. Fakultät für Biologie, LS für Experimentelle Ökologie, Universität Bielefeld, Bielefeld, Germany

    Wolfram Beyschlag

  3. FB Biologie, Abt. Allgemeine Botanik, TU Kaiserslautern, Erwin-Schrödinger-Str., Kaiserslautern, 67663, Germany

    Burkhard Büdel

  4. Cardiff School of Biosciences, University of Cardiff, Cardiff, United Kingdom

    Dennis Francis

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Nagata, T. (2010). A Journey with Plant Cell Division: Reflection at My Halfway Stop. In: Lüttge, U., Beyschlag, W., Büdel, B., Francis, D. (eds) Progress in Botany 71. Progress in Botany, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02167-1_1

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