Dynamic Light Scattering Study of Pig Vitreous Body
The phase behaviors and dynamical properties of pig vitreous body were studied by macroscopic observation of swelling behavior and dynamic light scattering under various conditions. From the observations of the dynamics of light scattered by the pig vitreous body under physiological condition, intensity autocorrelation functions that revealed two diffusion coefficients, D fast and D slow were obtained. We developed the theory for describing the density fluctuation of the entities in the vitreous gel system with sodium hyaluronate filled in the meshes of collagen fiber network. The dynamics of collagen and sodium hyaluronate explains two relaxation modes of the fluctuation. The diffusion coefficient of collagen obtained from D fast and D slow is very close to that in aqueous solution, which suggests the vitreous body is in the swollen state. Divergent behavior in the measured total scattered light intensities and diffusion coefficients upon varying the concentration of salt (NaCl and CaCl2) was observed. Namely, a slowing down of the dynamic modes accompanied by increased “static” scattered intensities was observed. This is indicative of the occurrence of a phase transition upon salt concentration.
KeywordsVitreous body Sodium hyaluronate Collagen Phase transition Critical phenomenon Dynamic light scattering
The work was partly supported by a Grant-in-Aid for Scientific Research on Priority Areas “Soft Matter Physics” (No. 19031024) and by a Grant-in-Aid for the Global COE Program, "Science for Future Molecular Systems" from the Ministry of Education, Culture, Science, Sports and Technology of Japan. Authors acknowledge Prof. Tokita for valuable discussion.
- 1.Berman ER, Voaden M (1970) In: Smelser GK (ed) Biochemistry of the Eye, Academic Press, London, p 373Google Scholar
- 2.Balazs EA (1968) In: McPherson A (ed) New and Controversial Aspects of Retinal Detachment, Academic Press: Philadelphia, vol 1, p 3Google Scholar
- 3.William ST (1968) Tras Amer Acad Opthalmol Otolaryngol 72:217Google Scholar
- 7.Worst JGF, Los LI (1995) Cisternal Anatomy of the Vitreous. Kugler Publications, AmsterdamGoogle Scholar
- 12.Flory PJ (1953) In:Principle of Polymer Chemistry, Cornell Univ. Press, Ithaca, pp 541–593Google Scholar
- 16.Berne BJ, Pecora R (1976) Dynamic Light Scattering, Plenum Press, New YorkGoogle Scholar
- 20.Tokita T, Fujita Y, Hikichi K (1984) Biorheology 21:751Google Scholar
- 21.Nickerson CS, Park J, Kornfield JA, Karageozian H (2008) J. Biomechanics 41:1823Google Scholar