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Dynamic Light Scattering Study of Pig Vitreous Body

  • Toyoaki Matsuura
  • Naokazu Idota
  • Yoshiaki Hara
  • Masahiko AnnakaEmail author
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 136)

Abstract

The phase behaviors and dynamical properties of pig vitreous body were studied by macroscopic observation of swelling behavior and dynamic light scattering under various conditions. From the observations of the dynamics of light scattered by the pig vitreous body under physiological condition, intensity autocorrelation functions that revealed two diffusion coefficients, D fast and D slow were obtained. We developed the theory for describing the density fluctuation of the entities in the vitreous gel system with sodium hyaluronate filled in the meshes of collagen fiber network. The dynamics of collagen and sodium hyaluronate explains two relaxation modes of the fluctuation. The diffusion coefficient of collagen obtained from D fast and D slow is very close to that in aqueous solution, which suggests the vitreous body is in the swollen state. Divergent behavior in the measured total scattered light intensities and diffusion coefficients upon varying the concentration of salt (NaCl and CaCl2) was observed. Namely, a slowing down of the dynamic modes accompanied by increased “static” scattered intensities was observed. This is indicative of the occurrence of a phase transition upon salt concentration.

Keywords

Vitreous body Sodium hyaluronate Collagen Phase transition Critical phenomenon Dynamic light scattering 

Notes

Acknowledgments

The work was partly supported by a Grant-in-Aid for Scientific Research on Priority Areas “Soft Matter Physics” (No. 19031024) and by a Grant-in-Aid for the Global COE Program, "Science for Future Molecular Systems" from the Ministry of Education, Culture, Science, Sports and Technology of Japan. Authors acknowledge Prof. Tokita for valuable discussion.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Toyoaki Matsuura
    • 1
  • Naokazu Idota
    • 2
  • Yoshiaki Hara
    • 1
  • Masahiko Annaka
    • 2
    Email author
  1. 1.Department of OphthalmologyNara Medical UniversityNaraJapan
  2. 2.Department of Chemistry, Faculty of SciencesKyushu UniversityFukuokaJapan

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