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An Application of Microcapsules Having Enzyme-degradable Gel Membrane to Cell Culture

  • Toshiaki DobashiEmail author
  • Michiru Koike
  • Kentaro Kobayashi
  • Yasuyuki Maki
  • Takao Yamamoto
  • Susumu Tanaka
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 136)

Abstract

Newly developed microcapsules having gelatin wall membrane was applied as a scaffold for suspension cell culture. The optimum preparation condition was determined, and the stability of the cultured human fibroblast cells using the microcapsules was examined at both protein and gene levels.

Keywords

Cell culture scaffold Microcapsule Fibroblast cell Gelatin gel membrane 

Notes

Acknowledgements

This work was partly supported by Grant-in-Aid for Science Research from The Ministry of Education, Culture, Sports, Science and Technology in Japan (grant no. 16540366) and (grand no. 20656129).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Toshiaki Dobashi
    • 1
    Email author
  • Michiru Koike
    • 1
  • Kentaro Kobayashi
    • 1
  • Yasuyuki Maki
    • 1
  • Takao Yamamoto
    • 1
  • Susumu Tanaka
    • 2
  1. 1.Department of Chemistry and Chemical Biology, Graduate School of EngineeringGunma University KiryuGunmaJapan
  2. 2.Department of Health and Nutrition, Faculty of Health and WelfareTakasaki University of Health and WelfareTakasakiJapan

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