Electrophoresis of Dyes and Proteins in Poly(Acrylamide) Gel Containing Immobilized Bilayer Membranes
Electrophoresis of dye stuffs and proteins in poly(acrylamide) gel containing immobilized bilayer membranes have been studied. Bilayer membranes of a polymerizable surfactant, dodecylglyceryl itaconate (DGI), can be immobilized in poly(acrylamide) gels, and the hybrid gels are first applied to a substrate of the poly(acrylamide) gel electrophoresis (PAGE). The bilayer-membranes-immobilized-gel (abbreviated as BM-gel) showed different separation behaviors from those by the conventional PAGE. The separation behavior of dye stuffs suggests that the bilayer membranes in the BM-gel work as a separator of the test molecules due to their hydrophilic/hydrophobic nature. Water-soluble proteins migrated faster in the BM-gels than in the simple poly(acrylamide) gels. Membrane proteins, on the other hand, did not move at all in the BM-gels probably because the protein molecules were entrapped firmly inside the bilayer membranes.
KeywordsElectrophoresis Bilayer mambranes Gel Poly(acrylamide) Proteins
The authors thank the financial support of the Grant-in-Aid for Scientific Research (No. 17655071) from Ministry of Education, Culture, Sports, Science, and Technology, Japan. G. M. acknowledges also to Shimadzu Science Foundation, and Shiseido Science Foundation, Japan for their financial support. We also appreciate Professor Naoki Kamo of Hokkaido University for his kind gift of the sample of bacteriorhodopsin.