Abstract
Proteome level information is necessary to understand the function of specific cell types and their roles in health and disease. Proteomics is a rapidly developing field with a wide range of applications in wound healing. The ability to use proteomics to assess the wound healing process would have many benefits, including earlier evidence of healing and better understanding of how different treatments affect the wound at the protein level. The basis of what is known about the chronic wound proteome is based on results from a broad collection of studies utilizing a number of different proteomic techniques on fluids and tissues from wounds with different etiologies. The identification of biomarkers associated with healing or delayed healing in chronic wounds could have great significance in the use of current treatments, as well as in the development of new therapeutic interventions.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Tyers, M., Mann, M.: From genomics to proteomics. Nature 422, 193–197 (2003)
ENCODE. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature 447(7146), 799–816 (2007)
Northrup, R.B., Connor, A.N.: Introduction. In: Neuman, M.R. (ed.) Introduction to Molecular Biology, Genomics and Proteomics for Biomedical Engineers, pp. 1–11. CRC Press Taylor and Francis Group, Boca Raton (2009)
Wagner, P.D., Srivastava, S.: The Promise of Proteomics: Biology, Applications, and Challenges. In: Srivastava, S. (ed.) Informatics in Proteomics, pp. 1–15. CRC Press Taylor and Francis Group, Boca Raton (2005)
Nanney, L.B., Caldwell, R.L., et al.: Novel approaches for understanding the mechanisms of wound repair. Journal of Investigative Dermatology Symposium Proceedings 11, 132–139 (2006)
Wysocki, A.B.: Wound fluids and the pathogenesis of chronic wounds. J. WOCN 23, 283–290 (1996)
Moseley, R., Hilton, J.R., et al.: Comparison of oxidative stress biomarker profiles between acute and chronic wound environments. Wound Repair Regen. 12, 419–429 (2004)
James, T.J., Hughes, M.A., et al.: Simple biochemical markers to assess chronic wounds. Wound Repair Regen. 8, 264–269 (2008)
Trengove, N.J., Langton, S.R., et al.: Biochemical analysis of wound fluid from nonhealing healing chronic leg ulcers. Wound Repair Regen. 4(2), 234–239 (1996)
Northrup, R.B., Connor, A.N.: Some Instrumental Methods Used in Genomics, Proteomics, and Forensic Science. In: Neuman, M.R. (ed.) Introduction to Molecular Biology, Genomics and Proteomics for Biomedical Engineers, pp. 283–327. CRC Press Taylor and Francis Group, Boca Raton (2009)
Jaynes, C.D., Fries, K., Brogan, M., Karch, J.E., Baird, K., Edsberg, L.E.: Development of a protocol for biochemical analysis of wound fluids. Acute Care Perspectives 12(1), 11–15 (2003)
Grinnell, F., Ho, C.-H., Wysocki, A.: Degradation of fibronectin and vitronectin in chronic wound fluid: Analysis by cell blotting, immunoblotting, and cell adhesion assays. J. Invest. Dermatol. 98, 410–416 (1992)
Moses, M.A., Marikovsky, J.W., et al.: Temporal study of the activity of matrix metalloproteinases and their endogenous inhibitors during healing. Journal of Cellular Biology 60, 379–386 (1996)
Schmidtchen, A.: Chronic ulcers: A method for sampling and analysis of wound fluid. Acta. Derm. Venereol. 79, 291–295 (1999)
Rasik, A.M., Shukla, A.: Antioxidant status in delayed healing type of wounds. Int. J. Exp. Path. 81, 257–263 (2000)
Chen, G., Beer, D.G.: Protein Expression Analysis. In: Srivastava, S. (ed.) Informatics in Proteomics, pp. 227–254. CRC Press Taylor and Francis Group, Boca Raton (2005)
Fernandez, M.L., Broadbent, G.K., et al.: Development of an enhanced proteomic method to detect prognostic and diagnostic markers of healing in chronic wound fluid. British Journal of Dermatology 158, 281–290 (2008)
Tarran, S.L.S., Craft, G.E., et al.: The use of proteomics to study wound healing: a preliminary study for forensic estimation of wound age. Medicine, Science, and the Law 47(2), 134–140 (2007)
Oh, J.E., Krapfenbauer, K., Lubec, G.: Proteomic identification of collagens and related proteins in human fibroblasts. Amino Acids 27, 305–311 (2004)
Volden, G., Thorsrud, A.K., et al.: Biochemical composition of suction blister fluid determined by high resolution multicomponent analysis (capillary gas chromatography-mass spectrometry and two-dimensional electrophoresis). The Journal of Investigative Dermatology 75, 421–424 (1980)
Macdonald, N., Cumberbatch, M., et al.: Proteomic analysis of suction blister fluid isolated from human skin. Clinical and Experimental Dermatology 31, 445–448 (2006)
Chojnacki, C., Steinsstraber, L., et al.: Proteome analysis of chronic wound fluids via MudPIT. In: 11th Annual Meeting on Surgical Research, Saar-brucken, Germany, Langenbeck’s Archives of Surgery, November 2007, vol. 392, pp. 769–838 (2007)
Pollins, A.C., Friedman, D.B., et al.: Proteomic investigation of human burn wounds by 2D-difference gel electrophoresis and mass spectrometry. Journal of Surgical Research 142, 143–152 (2007)
Aggarwal, K., Choe, L.H., et al.: Shotgun proteomics using the iTRAQ isobaric tags. Briefings in Functional Genomics and Proteomics 5(2), 112–120 (2006)
Trengove, N., Bielefeldt-Ohmann, H., et al.: Cytokine profiles of wound fluid from chronic leg ulcers. Wound Repair and Regeneration 2(3), 228 (1994)
Trengove, N.J., Bielefeldt-Ohmann, H., Stacey, M.C.: Mitogenic activity and cytokine levels in non-healing and healing chronic leg ulcers. Wound Rep. Reg. 8, 13–25 (2000)
Barone, E.J., Yager, D.R., et al.: Interleukin-1alpha and collagenase activity are elevated in chronic wounds. Plastic and Reconstructive Surgery 102, 1023–1027 (1998)
Harris, I.R., Yee, K.C., et al.: Cytokine and protease levels in healing and non-healing venous leg ulcers. Experimental Dermatology 4, 342–349 (1995)
Aiba-Kojima, E., Tsuno, N.H., et al.: Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Wound Rep. Regen. 15, 511–520 (2007)
Nwomeh, B.C., Liang, H.-X., et al.: MMP-8 is the predominant collagenase in healing wounds and nonhealing ulcers. Journal of Surgical Research 81, 189–195 (1999)
Rayment, E.A., Upton, Z., Shooter, G.K.: Increased matrix metalloproteinase-9 (MMP-9) activity observed in chronic wound fluid is related to the clinical severity of the ulcer. British Journal of Dermatology 158, 951–961 (2008)
Yager, D.R., Zhang, L.-Y., et al.: Wound fluids from human pressure ulcers contain elevated matrix metalloproteinase levels and activity compared to surgical wound fluids. J. Invest. Dermatol. 107, 743–748 (1996)
Ladwig, G.P., Robson, M.C., et al.: Ratios of activated matrix metalloproteinase-9 to tissue inhibitor of matrix metalloproteinase-1 in wound fluids are inversely correlated with healing pressure ulcers. Wound Rep. Reg. 10, 26–37 (2002)
Trengove, N.J., Stacey, M.C., et al.: Analysis of acute and chronic wound environments: the role of proteases and their inhibitors. Wound Rep. Reg. 7, 442–452 (1999)
Bullen, E.C., Long, M.T.: Tissue inhibitor of metalloproteinases-1 is decreased and activated gelatinases are increased in chronic wounds. J. Invest. Dermatol. 104, 236–240 (1995)
Nwomeh, B.C., Liang, H.-X., et al.: Dynamics of the matrix metalloproteinases MMP-1 and MMP-8 in acute open human dermal wounds. Wound Rep. Reg. 6, 127–134 (1998)
Cook, H., Stephns, P., et al.: Defective extracellular matrix reorganization by chronic wound fibroblasts is associated with alterations in TIMP-1, TIMP-2, and MMP-2 activity. J. Invest. Dermatol. 115, 225–233 (2000)
Lobmann, R., Ambrosch, A., et al.: Expression of matrix-metalloproteinases and their inhibitors in the wounds of diabetic and non-diabetic patients. Diabetologia 45, 1011–1016 (2002)
Mouës, C.M., van Toorenenbergen, A.W., et al.: The role of topical negative pressure in wound repair: Expression of biochemical markers in wound fluid during wound healing. Wound Rep. Reg. 16, 488–494 (2008)
Kilpadi, D.V., Stechmiller, J.K., et al.: Composition of wound fluid from pressure ulcers treated with negative pressure wound therapy using V.A.C. therapy in home health or extended vare patients: A pilot study. Wounds 18(5), 119–126 (2006)
Baker, E.A., Leaper, D.J.: Profiles of matrix metalloproteinases and their tissue inhibitors in intraperitoneal drainage fluid: Relationship to wound healing. Wound Rep. Reg. 11, 268–274 (2003)
Baker, E.A., Leaper, D.J.: Proteinases, their inhibitors, and cytokine profiles in acute wound fluid. Wound Rep. Reg. 8, 392–398 (2000)
Galkowski, H., Wojewodzka, U., et al.: Chemokines, cytokines, and growth factors in keratinocytes and dermal endothelial cells in the margin of chronic diabetic foot ulcers. Wound Repair Regeneration 14, 558–565 (2006)
Stojadinovic, O., Brem, H., et al.: Molecular pathogenesis of chronic wounds. American Journal of Pathology 167, 59–69 (2005)
Leber, T.M., Balkwill, F.R.: Zymography: A single-step staining method for quantitation of proteolytic activity on substrate gels. Anal. Biochem. 249(1), 24–28 (1997)
Wysocki, A.B., Staiano-Coico, L., et al.: Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP2- and MMP-9. J. Invest. Dermatol. 101, 64–68 (1993)
Wysocki, A.B., Kusakabe, A.O., et al.: Temporal expression of urokinase plasmi-nogen activator, plasminogen activator inhibitor and gelatinase-B in chronic wound fluid switches from a chronic to acute wound profile with progression to healing. Wound Rep. Reg. 7, 154–165 (1999)
Brem, H., Stojadinovic, O., et al.: Molecular markers in patients with chronic wounds guide surgical debridement. Mol. Med. 13(1-2), 30–39 (2007)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Berlin Heidelberg
About this chapter
Cite this chapter
Edsberg, L.E. (2009). Proteomic Approaches for Studying the Phases of Wound Healing. In: Gefen, A. (eds) Bioengineering Research of Chronic Wounds. Studies in Mechanobiology, Tissue Engineering and Biomaterials, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00534-3_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-00534-3_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-00533-6
Online ISBN: 978-3-642-00534-3
eBook Packages: EngineeringEngineering (R0)