Abstract
The previous chapter shows how small-angle X-ray Diffraction can be used to study the organization of collagen fibres in tissue, proposing this technique as a diagnosis tool. In this chapter, synchrotron small-angle X-ray diffraction (SAXD) by using high-angle and temporal resolution is presented as an essential tool in structural functional studies of skeletal muscle tissues. SAXD studies of muscle fibres involve the combination of mechanical and diffraction methods and provide insights into the molecular mechanisms responsible for the generation of force and motion in active muscle. These studies are made possible because of the highly ordered arrangement of the contractile proteins, myosin and actin, in the sarcomere, the smallest functional repeating unit of the muscle cell. The possibility to collect diffraction diagrams with high angular and temporal resolutions at modern third-generation synchrotron radiation sources together with new data processing algorithms together and two-dimensional photon counting detectors allow structural and functional studies of live muscle tissues. The review covers the basics of X-ray small-angle diffraction, instrumentation and mathematical methods used in data analysis. A general description of each of these points has been presented in Chap.1 and 2. It provides new results on the axial disposition of the myosin heads and their interpretation from analysing the interference fringes that carve the diffraction orders into clusters of peaks.
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References
Huxley H.E.R., Faruqi A.R., Bordas J., Koch M.H.J., Milch J.R. Nature 284, 140 (1980).
Huxley H.E.R., Simmons M., Faruqi A.R., Kress M., Bordas J., Koch M.H. J. Pro. Natl. Acad. Sci. USA 78, 2297 (1981).
Huxley H.E.R., Simmons M., Faruqi A.R., Kress M., Bordas J., Koch M.H.J. J. Mol. Biol. 158, 637 (1982).
Huxley H.E.R., Simmons M., Faruqi A.R., Kress M., Bordas J., Koch M.H.J. J. Mol. Biol. 169, 469 (1983).
Rosenbaum G., Holmes K.C., Witz J. Nature 230, 434 (1971).
Holmes K.C., Rosenbaum G.J. Synchrotron Rad. 5, 147 (1998).
Squire J. The Structural Basis of Muscle Contraction. Plenum Press, New York and London (1981).
Kabsch W., Mannherz E.G., Suck D., Pai E.F., Holmes K.C. Nature 347, 37 (1990).
Rayment I., Rypniewski W.R., Schmidt-Báse K., Smith R., Tomchick D.R., Benning M.M., Winklemann D.A., Wesenberg G., Holden H.M. Science 261, 50 (1993).
Bordas, J., Diakun G.P., Diaz F.G., Harries J.E., Lewis R.A., Lowy J., Mant G.R., Martin-Fernandez M.-L. Towns-Andrews E. J. Mus. Res. Cell Mot. 14, 311 (1993).
Irving M., Lombardi V., Piazzesi G., Ferenczi M.A. Nature 357, 156 (1992).
Irving M., Allen T.S., Sabido-David C., Craik J.S., Brandmeier B.D., Kendrick-Jones J., Corrie J.E.T., Trentham D.R., Goldman Y.E. Nature 375, 688 (1995).
Dobbie I., Linari M., Piazzesi G., Reconditi M., Koubassova N., Ferenczi M.A., Lombardi V., Irving M. Nature, 396, 383 (1998).
Piazzesi G., Reconditi M., Linari M., Lucii L., Sun Y.B., Narayanan T., Boesecke P., Lombardi V., Irving M. Nature 145, 659 (2002).
Martin-Fernandez M.L., Bordas J., Diakun G., Harries J., Lowy J., Mant G.R., Svensson A. and Towns-Andrews E.J. Mus. Res. Cell Mot. 15, 319 (1994).
Onuki H., Elleaume P. et al., Undulators, Wigglers, and Their Applications, ISBN 0-415-28040-0, 69 (2003).
Walker R.P. Insertion Devices: Undulators and Wigglers, (98-04) CERN Summer School, p:129–185.
X-ray data booklet, Sect. 2, p:2-1–2-16 (2001).
Chubar O., Elleaume P., Accurate and Efficient Computation of Synchrotron Radiation in the Near Field Region, EPAC98 Conference Proceedings Stockholm, 1177(1998).
Gabriel A. Rev. Sci. Instrum. 48(10), 1303 (1977).
Hendrix J., Koch M.H.J., Bordas J. J. Appl. Cryst. 12, 467 (1979).
Worgan J.S., Lewis R., Fore N.S., Sumner I.L., Berry A., Parker B., d’Annunzio F., Martin-Fernandez M.L., Towns-Andrews E., Harries J.E., Mant G.R., Diakun G.P., Bordas J. Nuc. Instrum. Meth. Phys. Res. A291 447(1990).
Lewis R.A., Berry A., Hall C.J., Helsby W.I., Parker B. Nucl. Instr. Methd. A454, 165 (2000).
Berry A., Helsby W.I., Parker B.T., Hall C.J., Buksh P.A., Hill A., Clague N., Hillon M., Corbett G., Clifford P., Tidbury A., Lewis R.A., Cernik B.J., Barnes P., Derbyshire G.E. Nucl. Instr. Meth. A513 (1–2), 260 (2003).
Helsby W.I., Berry A., Buksh P.A., Hall C.J., Lewis R.A. Nucl. Instrum. Meth., A510 (1–2), 138 (2003).
Huxley A.F., Niedergerke R. Nature 173, 971 (1954).
Huxley E.H., Hansen J. Nature 173, 973 (1954).
Knappeis G.G., Carlsen F. J. Cell. Biol. 13, 323 (1962).
Huxley H.E.J. Biophys. Acta 12, 387 (1957).
Egelmann E.H., DeRosier D.J. J. Mol Biol. 166, 623 (1983).
Hanson J., Lowey J. J. Mol. Biol. 6, 46 (1963).
Huxley H.E., Brown W. J. Mol. Biol. 30, 383 (1967).
Milligan R.A., Flicker P.F. J. Cell Biol. 105, 29 (1987).
Hanson J. Proc. Roy. Soc. Lond. (Biol.) 183, 39–58 (1973).
Lowey S. Subunits in Biological systems- Part A, S.N. Timasheff, G.D. Fasman and M. Dekker (Eds.). New York, 201 (1971).
Knight P., Trinick J.A. J. Mol. Biol. 177, 461 (1984).
Morgan M., Perry S.V., Ottaway J. Biochem. J. 177, 687 (1976).
Kensler R.W., Stewart M. J. Cell Biol. 96, 1797 (1986).
Squire J.M. Nature 233, 457 (1971).
Squire J.M. J. Mol. Biol. 72, 125 (1972).
Vainshtein B.K. Diffraction of X-Rays by Chain Molecules. Elsevier Publishing Company, London (1966).
Klug AQ., Crick F.C.H., Wyckoff H.W. Acta Cryst 11, 199 (1958).
Juanhuix J., Bordas J., Campmany J., Svensson A., Bassford M.L., Narayanan T. Biophys. J. 80, 1429 (2001).
Juanhuix J. Estructura molecular I funció dels músculs vius, PhD thesis, Autonomous University of Barcelona, Bellaterra, Spain (2001).
Bliss N., Bordas J., Fell B.S., Harris N.W., Helsby W.I., Mant G.R., Towns- Andrew E. Rev. Sci. Inst. 66(2), 1311 (1995).
Boesecke P., Diat O., Rasmussen B. Rev. Sci. Instrum. 66, 1636 (1995).
Koch M.H.J., Bendall P. J. Proc. Digital Equipment Computer User Soc. 13 (1981).
Mant G., Bordas J., private communications.
Hammersley A.P., Svensson S.O., Thompson A. Nucl. Instr. Meth. A346, 312 (1994).
Press W.H., Flannery B.P., Teukolsky S.A., Vetterling W.T. Numerical Recipes in Fortran 77 , 2nd Ed. The Art of Scientific Computing. Cambridge University Press, Cambridge (1992).
Miles C.V. Macromolecular Modelling Of Muscle Structure: A Comparison With X-Ray Diffraction Results, PhD thesis, University of Leciester, Leicester, UK (1997).
Bordas J., Lowy L., Svensson A., Harries J.E., Diakun G.P., Gandy J., Miles C.V., Mant G.R., Towns-Andrews E. Biophys. J. 68, 99S (1995).
Bordas J., Svensson A., Rothery M., Lowy J., Daikun G.P., Boesecke P., Biophys. J. 77, 3197 (1999).
Gordon A.M., Huxley A.F. J. Physiol. 184, 170 (1966).
Linari M., Piazzesi G., Dobbie I., Koubassova N., Reconditi M., Narayanan T., Diat O., Irving M., Lombardi V. Proc. Natl. Acad. Sci. USA 97, 7226 (2000).
Bordas J., Mant G.R., Diakun G.P., Nave C. J. Cell Biol. 105, 1311 (1987).
Huxley H.E., Reconditi M., Stewart A., Irving T. Biophys. J. 84, 139a (2003).
Reconditi M., Linari M., Lucii L., Stewart A., Sun Y.B., Boesecke P., Narayanan T., Fischetti R.F., Irving T., Piazzesi G., Irving M., Lombardi V. Nature, 428, 578 (2004).
Bassford M.L. Modelling the molecular structure of muscle with comparison to x-ray diffraction data, PhD thesis, University of Leicester, Leicester, UK (2001).
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Svensson, A., Bordas, J., de la Cuesta, F. (2009). X-Ray Diffraction from Live Muscle Fibres. In: Gomez, M., Nogales, A., Garcia-Gutierrez, M., Ezquerra, T. (eds) Applications of Synchrotron Light to Scattering and Diffraction in Materials and Life Sciences. Lecture Notes in Physics, vol 776. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95968-7_14
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