Abstract
In spite of the recent advances in the X-Ray crystallography and nuclear magnetic resonance techniques, the determination of the quaternary structure of large protein complexes is still a challenge in molecular biology and biological sciences. In this respect, small-angle X-ray scattering (SAXS) is a key technique, enabling the determination of the possible structural conformation of complexes in an almost native state. Despite of this book being devoted to scattering techniques by synchrotron radiation, in this chapter we present two examples of application of laboratory-based SAXS to protein solution. The fundaments of the technique are obviously the same and have been deeply described in Chap. 2. In this chapter, we will introduce the application of SAXS to protein solution. Special emphasis is done on data reduction and absolute units calibration. As an example to illustrate the power of this technique, two new data sets for two protein complexes will be presented. This will show how high-quality SAXS data combined with advanced model strategies enables the determination of the quaternary structure of protein complexes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Voet D., Voet J.G., Pratt C.W. Fundamentals of Biochemistry. Wiley (2006).
Grossmann J.G. J. Appl. Cryst. 40, 217–222 (2007).
Svergun D.I., Koch M.H. Rep. Prog. Phys. 66, 1735–1782 (2003).
Pedersen J.S. J. Appl. Cryst. 37, 369–378 (2004).
Pedersen J.S. Hansen S., Bauer R. Eur. Biophys. J. 22, 379–389 (1994).
Glatter O. Acta Phys. Austriaca 52, 243–256 (1980).
Glatter O. J. App. Cryst. (14), 101–108 (1981).
Pedersen J. S. Analysis of small-angle scattering data from colloids and polymer solutions: modeling and least-squares fitting. Adv. Colloid Interface Sci., 70, 171–210 (1997).
Svergun D.I., Barberato C., Koch M.H. J. Appl. Cryst. 28, 768–773 (1995).
Petoukhov M.V., Svergun D.I. Curr. Opin. Struct. Biol. 17(5), 562–571 (2007).
Dong M., Xu S., Oliveira C.L.P., Pedersen J.S., Thiel S., Besenbacher F., Vorup-Jensen T.J. Immun. 178 (5), 3016–3022 (2007).
Svergun D.I. Biophys. J. 76, 2879–2886 (1999).
Petoukhov M.V., Svergun D.I. Biophys. J. 89, 1237–1250 (2005).
Andersen C.B.F., Ballut L., Johansen J.S., Chamieh H., Nielsen K.H., Oliveira C.L.P., Pedersen J.S., Seraphin B., Le Hir H., Andersen G.R. Science 313 (5795) 1968–1972 (2006).
Chamieh H., Ballut L., Bonneau F., Le Hir H. Nat. Struct. Mol. Biol. 15, 85–93 (2008).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Oliveira, C., Vorup-Jensen, T., Andersen, C., Andersen, G., Pedersen, J. (2009). Discovering New Features of Protein Complexes Structures by Small-Angle X-Ray Scattering. In: Gomez, M., Nogales, A., Garcia-Gutierrez, M., Ezquerra, T. (eds) Applications of Synchrotron Light to Scattering and Diffraction in Materials and Life Sciences. Lecture Notes in Physics, vol 776. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95968-7_11
Download citation
DOI: https://doi.org/10.1007/978-3-540-95968-7_11
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-95967-0
Online ISBN: 978-3-540-95968-7
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)