Abstract
Under iron limitation, most microorganisms synthesize siderophores which form soluble complexes with Fe+++ with very high formation constants. Thus iron is solubilized, and subsequently taken up by microbial transport systems which recognize the various Fe-siderophores.
In this chapter, common fungal siderophore structures are presented, and useful methods are described for the extraction, isolation and identification of hydroxamates in particular. As an example for chemical assays, the chrome azurol S (CAS) assay is selected for testing for the presence of hydroxamate siderophores, e.g., in culture filtrates. The use of adsorption on AmberliteR XAD-2 or -16 columns is outlined for isolation of hydroxamates from such matrices. Further separation and identification of fungal siderophores requires the application of various analytical methods, such as HPLC, mass spectrometry and NMR spectroscopy.
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Haselwandter, K., Winkelmann, G. (2009). Siderophores of Mycorrhizal Fungi: Detection, Isolation and Identification. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_25
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DOI: https://doi.org/10.1007/978-3-540-95894-9_25
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