Abstract
Biotechnological production of plant-based secondary metabolites by a cell culture-based bioprocess generally suffers from an inherent disadvantage of low yield. In order to overcome this, elicitation is one of the most promising yield-enhancement strategies. Biotic elicitors, especially those of a fungal origin, are known to increase the production of secondary metabolites in plant cell suspension cultures. So far, mostly pathogenic fungi-based elicitor preparations have been widely used for this purpose. This chapter provides details of the application of a symbiotic arbuscular mycorrhiza-like fungus, Piriformospora indica, as an effective elicitor to optimize the concentration of different elicitor preparations and the time of incubation for enhanced production of the anticancer drug podophyllotoxin by cell suspension cultures of Linum album.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Baldi A, Bisaria VS, Srivastava AK (2007) Biotechnological approaches for production of some plant based chemotherapeutics. In: Kayser O, Quax WJ (eds) Medicinal plant biotechnology — From basic research to industrial applications, Wiley-VCH Verlag, Weinheim, pp 117–156
Chattopadhyay S, Srivastava AK, Bhojwani SS, Bisaria VS (2001) Development of suspension culture of Podophyllum hexandrum for the production of podophyllotoxin. Biotechnol Lett 23:2063–2066
Chattopadhyay S, Srivastava AK, Bhojwani SS, Bisaria VS (2002a) Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor. J Biosci Bioeng 93:215–220
Chattopadhyay S, Srivastava AK, Bisaria VS (2002b) Optimization of culture parameters for production of podophyllotoxin in suspension culture of Podophyllum hexandrum. Appl Biochem Biotechnol 102/103:381–393
Chattopadhyay S, Bisaria VS, Bhojwani SS, Srivastava AK (2003a) Enhanced production of podophyllotoxin by fed-batch cultivation of Podophyllum hexandrum. Can J Chem Eng 81:1–8
Chattopadhyay S, Bisaria VS, Srivastava AK (2003b) Enhanced production of podophyllotoxin by Podophyllum hexandrum using in situ cell retention bioreactor. Biotechnol Prog 19:1026–1028
Chattopadhyay S, Mehra RS, Srivastava AK, Bhojwani SS, Bisaria VS (2003c) Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures. Appl Microbiol Biotechnol 60:541–546
Farakya S, Bisaria VS, Srivastava AK (2004) Biotechnological aspects of the production of the anticancer drug podophyllotoxin. Appl Microbiol Biotechol 65:504–519
Farkya S, Julka A, Mehra R, DattaV, Srivastava AK, Bisaria VS (2005) Enhanced production of secondary metabolites by biotic elicitors in plant cell suspension cultures. Presented at 5th Asia Pacific Biochemical Engineering Conference, May 15–19, 2005, Jeju Island, Korea
Hadimani SB, Tanpure RP, Bhat SV (1996) Asymmetric total synthesis of (-) Podophyllotoxin. Tetrahedron Lett 37: 4791–4794
Imbert F (1998) Discovery of podophyllotoxins. Biochimie 80:207–222
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay of tobacco tissue cultures. Physiol Plant 15:473–497
Stahelin HF, Wartburg AV (1991) The chemical and biological route from podophyllotoxin glucoside to etoposide. Cancer Res 51:5–15
Van Uden W (1992) The production of podophyllotoxin and related cytotoxic lignans by plant cell cultures. Ph.D. Thesis, University of Groningen, Groningen, The Netherlands
Varma A, Franken P (1997) European Patent Office, Munich, Germany, Patent No. 97121440.8-2105, Nov 1998
World Conservation Monitoring Centre (2001) Checklist of CITES species: a reference to the appendices to the Convention on international trade in endangered species of wild fauna and flora, CITES Secretariat/World Conservation Monitoring Centre, Chatelâine-Genève
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Baldi, A., Srivastava, A.K., Bisaria, V.S. (2009). Fungal Elicitors for Enhanced Production of Secondary Metabolites in Plant Cell Suspension Cultures. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_23
Download citation
DOI: https://doi.org/10.1007/978-3-540-95894-9_23
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-95893-2
Online ISBN: 978-3-540-95894-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)