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Nanoscience pp 595–638Cite as

Mass Spectrometry

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Abstract

For twenty years or so now, mass spectrometry has been used to get exact measurements of the mass of biological molecules such as proteins, nucleic acids,oligosaccharides, and so on. Over the past ten years, this technology has followed the trend toward miniaturisation and the samples required can be much smaller. In particular, the nanoelectrospray source (online or by needle) allow one to work at flow rates of a few tens of nanolitres/min. There are many applications, both in the field of proteomics and in the analysis of protein structure, dynamics, and interactions. Combining this source with nanoHPLC, complex mixtures only available in small quantities can be separated and analysed online. There are also some advantages over conventional HPLC, despite a set of constraints related to the small dimensions and low flow rates. Combining capillary electrophoresis with the electrospray source also gives useful results, with its own set of advantages and constraints. Finally, developments are currently underway to combine this source with chips, providing a means of separation and analysis online.

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Authors and Affiliations

  1. Laboratoire analyse et modélisation pour la biologie et l’environnement, Université d’Evry-val-d’Essonne Boulevard F. Mitterrand, 91025, Evry Cedex, France

    D. Pflieger

  2. CEA, 17 rue des Martyrs, 38054, Grenoble Cedex, France

    E. Forest

  3. Ecole supérieure de physique et de chimie industrielles, 10 rue Vauquelin, 75231, Paris Cedex 05, France

    J. Vinh

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  1. D. Pflieger
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  2. E. Forest
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  3. J. Vinh
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Correspondence to D. Pflieger .

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  1. LETI-MINATEC, CEA, rue des martyrs 17, Grenoble CX 9, 38054, France

    Patrick Boisseau

  2. Université d'Evry, bd. F. Mitterrand, Evry CX, 91025, France

    Philippe Houdy

  3. Dépt. Sciences des Matériaux, Université d'Evry, rue du père Jarlan, Evry CX, 91025, France

    Marcel Lahmani

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Pflieger, D., Forest, E., Vinh, J. (2009). Mass Spectrometry. In: Boisseau, P., Houdy, P., Lahmani, M. (eds) Nanoscience. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-88633-4_10

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