Abstract
Whereas the directed evolution of stereoselective enzymes provides a useful tool in asymmetric catalysis, generality cannot be claimed because enzymes as catalysts are restricted to a limited set of reaction types. Therefore, a new concept has been proposed, namely directed evolution of hybrid catalysts in which proteins serve as hosts for anchoring ligand/transition metal entities. Accordingly, appropriate genetic mutagenesis methods are applied to the gene of a given protein host, providing after expression a library of mutant proteins. These are purified and a ligand/transition metal anchored site-specifically. Following en masse ee-screening, the best hit is identified, and the corresponding mutant gene is used as a template for another round of mutagenesis, expression, purification, bioconjugation, and screening. This allows for a Darwinian optimization of transition metal catalysts.
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Abbreviations
- BINOL:
-
Binaphthol
- BSA:
-
Bovine serum albumin
- CAST:
-
Combinatorial active-site saturation test
- cod:
-
1,5-Cyclooctadiene ee Enantiomeric excess
- epPCR:
-
Error-prone polymerase chain reaction
- ESI-MS:
-
Electro-spray ionization mass spectrometry
- ESI-TOF MS:
-
Electro-spray ionization time of flight mass spectrometry
- HSA:
-
Human serum albumin
- IPTG:
-
Isopropyl-β-d-thiogalactopyranoside
- ISM:
-
Iterative saturation mutagenesis
- L-BAPNA:
-
N-Benzoyl-l-arginine-p-nitroanilide
- LB-medium:
-
Luria–Bertani medium
- MALDI-TOF-MS:
-
Matrix assisted laser desorption ionization time of flight mass spectrometry
- MS:
-
Mass spectrometry
- PCR:
-
Polymerase chain reaction
- SDS PAGE:
-
Sodium dodecylsulfate polyacrylamide gel electrophoresis
- TB-medium:
-
Terrific broth
- WT:
-
Wild-type
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Reetz, M.T. (2009). Directed Evolution of Stereoselective Hybrid Catalysts. In: Ward, T.R. (eds) Bio-inspired Catalysts. Topics in Organometallic Chemistry, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-87757-8_4
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