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Microbial Megaplasmids pp 239–270Cite as

Megaplasmids of Aerobic Hydrogenotrophic and Carboxidotrophic Bacteria

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Part of the book series: Microbiology Monographs ((MICROMONO,volume 11))

Abstract

The hydrogen-oxidizing bacterium Ralstonia eutropha H16 and the carbon monoxide-oxidizing bacterium Oligotropha carboxidovorans OM5 carry key genetic determinants for their respective forms of lithoautotrophic metabolism on megaplasmids. In R. eutropha H16 genetic information for the H2-oxidizing system and for CO2 fixation via the Calvin—Benson—Bassham cycle is located on the 452-kb megaplasmid pHG1. In addition, pHG1 harbors clusters of genes for denitrification and for degradation of aromatic compounds. The 133-kb megaplasmid pHCG3 is the genetic basis for CO oxidation in O. carboxidovorans OM5. Aside from the aerobic CO dehydrogenase, pHCG3 also encodes Calvin cycle enzymes and a dimeric hydrogenase. In both cases there is an interdigitation of megaplasmid-based and chromosomally encoded functions, indicating that these megaplasmids are, although not strictly essential for viability, an integral part of the genome.

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Acknowledgments

The author is grateful to R. Cramm, H. Dobbek, T. Eitinger, B. Friedrich and O. Lenz for critical reading of the manuscript and to O. Lenz and B. Kusian for providing illustrations.

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  1. Institut für Biologie, Mikrobiologie, Humboldt-Universität zu Berlin, Chauseestr. 117, Berlin, 10115, Germany

    Edward Schwartz

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  1. Institut fuer Biologie, Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestr. 117, Berlin, 10115, Germany

    Edward Schwartz

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Schwartz, E. (2009). Megaplasmids of Aerobic Hydrogenotrophic and Carboxidotrophic Bacteria. In: Schwartz, E. (eds) Microbial Megaplasmids. Microbiology Monographs, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85467-8_11

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