Abstract
Cultivating microbes is enough of a challenge when one knows they are there: the microbial world is rife with examples of bugs that are clearly growing in nature, yet seem to be uncultivable despite our best efforts. While many of these clearly cultivable “uncultivables” defy our efforts, as a group these microbes may provide some valuable clues and lessons for those who would venture into the world of the search for extraterrestrial life. That is to say, careful consideration of how one might recognize and/or cultivate ET organisms might yield some insights into the more realistic question of how to find and cultivate life in earthly habitats – after all, our ability to cultivate microbes that we can clearly see are capable of growth has been disappointing to say the least. Thus, an open-minded approach to why our own earthly efforts have yielded such limited success might well be used to formulate a strategy for the cultivation of ET life, and perhaps more importantly, to improve our efforts to cultivate life from our own planet. And incidentally, should we be so fortunate as to ever obtain uncontaminated extraterrestrial samples, we will be ready!
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References
Baker BJ, Tyson GW et al (2006) Lineages of acidophilic Archaea revealed by community genomic analysis. Science 314:1933–1935
Beja O, Aravind L et al (2000) Bacterial rhodopsin: evidence for a new type of phototrophy in the sea. Science 289:1902–1906
Boetius A, Ravenschlag K et al (2000) A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407:623–626
Breznak JA, Leadbetter JR (2002) Termite gut spirochetes. In: Dworkin M, Falkow S, Rosenberg E, Schliefer KH, Stackebrandt E (eds) The Prokaryotes – an evolving electronic resource for the microbiological community. Springer, New York
Brownlie JC, O'Neill SL (2006) Wolbachia genomes: insights into an intracellular lifestyle. Curr Biol 15:R507–R509
D'Hondt S, Rutherford S, Spivack AJ (2002) Metabolic activity of subsurface life in deep-sea sediments. Science 295:2067–2070
D'Hondt S, Joergensen BB et al (2004) Distributions of microbial activities in deep subseafloor sediments. Science 306:2216–2221
Dorn ED (2005) Universal biosignatures for the detection of life. Computer Sciences. Pasadena, California Institute of Technology, California, p 123
Dorn ED, McDonald GD et al (2003) Principal component analysis and neural networks for detection of amino acid biosignatures. Icarus 166(2):403–409
Fenchel T, Finlay BJ (1995) Ecology and evolution in anoxic worlds. Oxford University Press, Oxford
Gomez-Consarnau L, Gonzalez JM et al (2007) Light stimulates growth of proteorhodopsin-containing marine Flavobacteria. Nature 445:210–213
Haygood MG, Tebo BM et al (1984) Luminous bacteria of a monocentrid fish (Monocentris-Japonicus) and 2 anomalopid fishes (Photoblepharon-Palpebratus and Kryptophanaron-Alfredi) – population sizes and growth within the light organs, and rates of release into the seawater. Mar Biol 78(3):249–254
Huber H, Hohn MI et al (2002) A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont. Nature 417:63–67
Inagaki F, Takai K et al (2003) Sulfurimonas autotrophica gen. nov., sp nov., a novel sulfur-oxidizing epsilon-proteobacterium isolated from hydrothermal sediments in the mid-Okinawa trough. Int J Syst Evol Micr 53:1801–1805
Inagaki F, Takai K et al (2004) Sulfrovum lithotrophicum gen.nov., sp. nov., a novel sulfur-oxidizing epsilon-proteobacterium isolated from hydrothermal sediments from the mid-Okinawa trough. Int J Syst Evol Microbiol 54:1477–1487
Kerr RA (2001) Life – potential, slow, or long dead? Science 294:1820–1821
Kerr RA (2002) Deep life in the slow, slow lane. Science 296:1056–1058
Margulis L (1981) Symbiosis in cell evolution. Freeman, San Francisco
Moran NA, Degnan PH (2006) Functional genomics of Buchnera and the ecology of aphid hosts. Mol Ecol 15:1251–1261
Morin JG, Harrington A et al (1975) Light for all reasons – versatility in behavioral repertoire of flashlight fish. Science 190(4209):74–76
Nealson K (2001) Searching for life in the universe: lessons from the Earth. Ann N Y Acad Sci 950:241–258
Nealson K, Berelson W (2009) Sedimentary habitats. In: Schaechter M (ed) Desk encyclopedia of microbiology. Academic, London, p 800
Nealson K, Berelson W (2003) Layered microbial communities and the search for life in the universe. Geomicrobiol J 20:451–462
Nealson KH, Conrad PG (1999) Life: past, present and future. Phil Trans R Soc Lond B 354:1–17
Nealson KH, Cox BL (2002) Microbial metal-ion reduction and Mars: extraterrestrial expectations? Curr Opin Microbiol 5:296–300
Nealson KH, Haygood MG et al (1984) Contribution by symbiotically luminous fishes to the occurrence and bioluminescence of luminous bacteria in seawater. Microb Ecol 10(1):69–77
Nealson KH, Tsapin A, Storrie-Lombardi M (2002) Searching for life in the Universe: unconventional methods for an unconventional problem. Int Microbiol 5:223–230
O'Neill SL, Giordano R et al (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci U S A 89:2699–2672
Olsen GJ, Woese CR et al (1994) The winds of (evolutionary) change: breathing new life into microbiology. J Bacteriol 176:1–6
Pace NR (1997) A molecular view of microbial diversity and the biosphere in a Yellowstone hot spring. Science 276:734–740
Schink B, Stams AJM (2002) Syntrophism among prokaryotes. In: Dworkin M (ed) The prokaryotes: an evolving electronic resource for the microbiological community. Springer, New York
Schippers A, Neretin LN et al (2005) Prokaryotic cells of the deep sub-seafloor biosphere identified as living bacteria. Nature 433:861–864
Sharma AK, Spudich JL et al (2006) Microbial rhodopsins: functional versatility and genetic mobility. Trends Microbiol 14:463–469
Shungu D, Valiant M et al (1983) Gelrite as an agar substitute in bacteriological media. Appl Environ Microbiol 46(4):840–845
Sneath PHA (1992) International code of nomenclature of bacteria: bacteriological code, 1990 revision. American Society Microbiology, Washington, DC
Stingl U, Radek R et al (2005) Endomicrobia: cytoplasmic symbionts of termite gut protozoa from a separate phylum of prokaryotes. Appl Environ Microbiol 71:1473–1479
Takai K, Hirayama H, Sakihama Y, Inagaki F, Yamato Y, Horikoshi K (2002) Isolation and metabolic characteristics of previously uncultured members of the order Aquifales in a subsurface gold mine. Appl Environ Microbiol 68:3046–3054
Takai K, Inagaki F et al (2003a) Isolation and phylogenetic diversity of members of previously uncultivated epsilon-proteobacteria in deep-sea hydrothermal fields. FEMS Microbiol Lett 218(1):167–174
Takai K, Kobayashi H et al (2003b) Deferribacter desulfuricans sp nov., a novel sulfur-, nitrate- and arsenate-reducing thermophile isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 53:839–846
Takai K, Nealson KH et al (2004) Hydrogenimonas thermophila gen. nov., sp nov., a novel thermophilic, hydrogen-oxidizing chemolithoautotroph within the epsilon-proteobacteria, isolated from a black smoker in a Central Indian Ridge hydrothermal field. Int J Syst Evol Microbiol 54:25–32
Venter JC, Remington K et al (2004) Environmental genome shotgun sequencing of the Sargasso sea. Science 304(5667):66–74
Waters E, Hohn MJ et al (2003) The genome of Nanoarchaeum equitans: insights into early archael evolution and derived parasitism. Proc Natl Acad Sci U S A 100:12984–12988
Whitman BW, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci U S A 95:6578–6583
Woese CR (2004) A new biology for a new century. Micorbiol Mol Biol Rev 68:173–186
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Nealson, K. (2009). Taking the Concept to the Limit: Uncultivable Bacteria and Astrobiology. In: Epstein, S. (eds) Uncultivated Microorganisms. Microbiology Monographs, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85465-4_9003
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