Abstract
This work focuses on the segmentation of axonal structures in digital images of organotypic slice co-cultures. An image processing chain is presented, which relies on anisotropic diffusion for preprocessing of the images and the intelligent scissors method for segmentation. This method requires manual user interaction to set the starting points. To overcome this drawback, the initial parameters for the intelligent scissors are automatically extracted from the images by a graph-based approach.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Heine C, Wegner A, Grosche J, et al. P2 receptor expression in the dopaminergic system of the rat brain during development. Neuroscience. 2007;149(1):165–81.
Pakura M, Schmitt O, Aach T. Segmentation and analysis of nerve fibers in histologic sections of the cerebral human cortex. Proc SSIAI. 2002; p. 62–6.
Zana F, Klein JC. Segmentation of vessel-like patterns using mathematical morphology and curvature evaluation. IEEE Trans Image Process. 2001;10(7):1010–9.
Jelinek HF, Cree MJ, Leandroa JJG, et al. Automated segmentation of retinal blood vessels and identification of proliferative diabetic retinopathy. J Opt Soc Am A. 2007;24(5):1448–56.
Mortensen EN, Barrett WA. Intelligent scissors for image composition. Proc SIGGRAPH. 1995; p. 191–8.
Weickert J. Anisotropic Diffusion in Image Processing. Teubner; 1998.
Chan TF, Osher S, Shen J. The digital TV filter and nonlinear denoising. IEEE Trans Image Process. 2001;10(2):231–41.
Braumann UD, Franke H, Hengstler J, et al. Graph-based quantification of astrocytes. Proc BVM. 2006; p. 379–83.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Scherf, N., Kuska, JP., Heine, C., Braumann, UD., Franke, H. (2008). Segmentation of Axonal Fibres in Tissue Slices. In: Tolxdorff, T., Braun, J., Deserno, T.M., Horsch, A., Handels, H., Meinzer, HP. (eds) Bildverarbeitung für die Medizin 2008. Informatik aktuell. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-78640-5_36
Download citation
DOI: https://doi.org/10.1007/978-3-540-78640-5_36
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-78639-9
Online ISBN: 978-3-540-78640-5
eBook Packages: Computer Science and Engineering (German Language)