Abstract
In this paper, we propose an autonomous molecular walking machine using DNA. This molecular machine follows a track of DNA equipped with many single-strand DNA stators arranged in a certain pattern. The molecular machine achieves autonomous walk by using a restriction enzyme as source of power. With a proposed machine we can control its moving direction and we can easily extend walking patterns in two or three dimensions. Combination of multiple legs and ssDNA stators can control the walking pattern. We designed and performed a series of feasibility study with molecular biology experiments.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Sherman, W.B., Seeman, N.C.: A Precisely Controlled DNA Biped Walking Device. Nano Lett. 4, 1203–1207 (2004)
Shin, J.-S., Pierce, N.A.: A Synthetic DNA Walker for Molecular Transport. J. Am. Chem. Soc. 126, 10834–10835 (2004)
Ye Tian, Yu He, Yi Chen, Peng Yin and Chengde Mao: A DNAzyme That Walks Processively and Autonomously along a One-Dimensional Track; Angew. Chem. Int. Ed. 2005, 44, 4355-4358
Bath, J., Green, S.J., Turberfield, A.J.: A free-running DNA motor powered by a nicking enzyme. Angew. Chem. Int. Ed. 44, 4358–4361 (2005)
Pei, R., Taylor, S.K., Stefanovic, D., Rudchenko, S., Mitchell, T.E., Stojanovic, M.N.: Behavior of Polycatalytic Assemblies in a Substrate- Displaying Matrix. J. Am. Chem. Soc. 128, 12693–12699 (2006)
Yin, P., Yan, H., Daniell, X.G., Turberfield, A.J., Reif, J.H.: A Unidirectional DNAWalker That Moves Autonomously along a Track. Angew. Chem. Int. Ed. 43, 4906–4911 (2004)
Benenson, Y., Paz-Elizur, T., Adar, R., Keinan, E., Livneh, Z., Shapiro, E.: Programmable and autonomous computing machine made of biomolecules. Nature 414, 430–434 (2001)
Verma, S., Eckstein, F.: Modified Oligonucleotides:Synthesis and Strategy for Users. Annu. Rev. Biochem. 67, 99–134 (1998)
Taylor, J.W., Schmidt, W., Cosstick, R., Okruszek, A., Eckstein, F.: The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA. Nucleic Acids Res. 13, 8749–8764 (1985)
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Sekiguchi, H., Komiya, K., Kiga, D., Yamamura, M. (2008). A Realization of DNA Molecular Machine That Walks Autonomously by Using a Restriction Enzyme. In: Garzon, M.H., Yan, H. (eds) DNA Computing. DNA 2007. Lecture Notes in Computer Science, vol 4848. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77962-9_6
Download citation
DOI: https://doi.org/10.1007/978-3-540-77962-9_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-77961-2
Online ISBN: 978-3-540-77962-9
eBook Packages: Computer ScienceComputer Science (R0)